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We narrowed to 60,203 results for: cloning

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Showing: 8001 - 8020 of 60203 results

  4. pCMV-dR8.2 dvpr

    Plasmid
    #8455
    Purpose
    2nd* generation lentiviral packaging plasmid. (*See comments section.) Can be used with 2nd and 3rd generation transfer vectors. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.
    Depositor
    Bob Weinberg
    Article
    Stewart et al RNA 2003 Apr;9(4):493-501.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    June 20, 2005
    Availability
    Academic Institutions and Nonprofits only
    8455-plasmid-map-sequence-id-370240

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  5. Lenti-multi-Guide

    Plasmid
    #85401
    Purpose
    Lentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neo
    Depositor
    Qin Yan
    Article
    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    Feb. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    85401-plasmid-map-sequence-id-322305

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  6. pGA-red-maxi

    Plasmid
    #196337
    Purpose
    Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening
    Depositor
    Paola Branduardi
    Article
    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly backbone
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196337-plasmid-map-sequence-id-393160

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  8. pCAGGS_3E5E_eGFP

    Plasmid
    #103054
    Purpose
    Encodes Ebola virus minigenome with EGFP reporter gene. Minigenome is under control of the CAG promoter
    Depositor
    Elke Mühlberger
    Article
    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do
    Insert
    Ebola virus (ebolavirus Zaire, Mayinga) minigenome
    Tags
    3E5E eGFP cloned into pCAGGS vector from pCDNA3.1…
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    103054-plasmid-map-sequence-id-197128

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  13. pCRISPomyces-2BD

    Plasmid
    #234428
    Purpose
    Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template
    Depositor
    Min-Kyu Oh
    Article
    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:
    Insert
    Modified Cas9 with polyaspartate linking
    Expression
    Bacterial
    Mutation
    Linked polyaspartate using glycine-serine linker
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234428-plasmid-map-sequence-id-468848

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  15. Tet-pLKO-neo

    Plasmid
    #21916
    Purpose
    3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.
    Depositor
    Dmitri Wiederschain
    Article
    Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    Aug. 22, 2009
    Availability
    Academic Institutions and Nonprofits only
    21916-plasmid-map-sequence-id-358974

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  16. pdCRISPomyces-2BD

    Plasmid
    #234429
    Purpose
    Plasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning template
    Depositor
    Min-Kyu Oh
    Article
    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:
    Insert
    Modified dCas9(D10A, H840A) with polyaspartate linking
    Expression
    Bacterial
    Mutation
    Linked polyaspartate using glycine-serine linker
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234429-plasmid-map-sequence-id-468849

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  19. pV1524

    Plasmid
    #111431
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)
    Depositor
    Gerald Fink
    Article
    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111431-plasmid-map-sequence-id-292191

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  20. pDG458

    Plasmid
    #100900
    Purpose
    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100900-plasmid-map-sequence-id-457690

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Showing: 8001 - 8020 of 60203 results