We narrowed to 17,686 results for: por

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pDONR221_SLC7A3

Plasmid

#132301

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Rebsamen et al Life Sci Alliance. 2022 Sep 16;5(11):e20

Insert

SLC7A3 (SLC7A3 Human)

Expression

Mammalian

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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FL MALT1 Catalytic Mutant in pET29b-Strep

Plasmid

#48969

Purpose

Expresses Full length catalytic mutant MALT1 in e.coli

Depositor

Guy Salvesen

Article

Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

Insert

MALT1 isoform A (MALT1 Human)

Tags

His and Strep

Expression

Bacterial

Mutation

Catalytic Mutant C464A

Promoter

T7

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only
TRE-UBC-Crry-T2A-GFP

Plasmid

#205452

Purpose

lentiviral plasmid for expression of mouse Crry

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

Crry (Cr1l Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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ZIKV_NASBA_32B

Plasmid

#75011

Purpose

Portion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sites

Depositor

James Collins, Alexander Green

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_NASBA_32B

Expression

Bacterial

Promoter

T7

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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ZIKV_NASBA_27B

Plasmid

#75010

Purpose

Portion of Zika Virus genome (KU312312: 1248-1594) containing sensor 27B trigger sequence and NASBA amplification sites

Depositor

James Collins, Alexander Green

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_NASBA_27B

Expression

Bacterial

Promoter

T7

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-F-API5

Plasmid

#157662

Purpose

Expresses API5 in mammalian cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Tags

FLAG tag

Expression

Mammalian

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF17 tail 400-1038

Plasmid

#134617

Purpose

Mammalian expression of mouse KIF1A 400-1038

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF17 (Kif17 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 399 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-GSDMD_NoCys-Myc

Plasmid

#201169

Purpose

Expression of GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D (Gsdmd Mouse)

Use

Retroviral

Tags

Myc

Mutation

C39A, C57A, C77A, C122A, C265A, C192A

Promoter

MESV

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9-Puro HLAG-2B-sgRNA

Plasmid

#182552

Purpose

Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

Depositor

Santiago Miriuka

Article

Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

Insert

hSpCas9-2A-Puro-HLAG-2B-sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

Cbh

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-API5

Plasmid

#157655

Purpose

Expresses API5 in E.coli cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Tags

His tag

Expression

Bacterial

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2 Notch1 LNG-6MT

Plasmid

#41738

Depositor

Raphael Kopan, Jeffrey Nye

Article

Kopan et al Proc Natl Acad Sci U S A. 1996 Feb 20;93

Insert

Notch-1 Lin-Notch-glp (LNG) repeat-containing construct (Notch1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

Contains murine Notch-1 leader peptide (aa 1-23) …

Available Since

Jan. 14, 2013

Availability

Academic Institutions and Nonprofits only
pCI-neo-(HA)3-ε

Plasmid

#198531

Purpose

Expression of HA-tagged AP-4 ε in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

Insert

AP-4 ε (AP4E1 Human)

Tags

triple HA tag

Expression

Mammalian

Mutation

silent substitutions in codons 235, 262, 905 and …

Promoter

CMV

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMRX-IP/SECFP-mATG5

Plasmid

#58744

Purpose

Expresses /SECFP-ATG5 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

autophagy related genes 5 (Atg5 Mouse)

Use

Retroviral

Tags

SECFP

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only
pT2EFCRET(#324)

Plasmid

#184059

Purpose

cyclofen-inducible CRE activation in zebrafish transient transgenic

Depositor

Sophie Vriz

Article

Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1

Insert

CRE-ERT2

Use

Zebrafish transient transgenesis

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Xl-EF1a

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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p.UTA.2.0 miR-27a-3p 3 perfect copies.

Plasmid

#82439

Purpose

Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p in three copies

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Insert

hsa-miR-27a-3p (MIR27A Human)

Mutation

Three copies hsa-miR-27a-3p Complementary region …

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pPN098

Plasmid

#91625

Purpose

Express sgRNA targeting human HCN1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

HCN1 (HCN1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN099

Plasmid

#91626

Purpose

Express sgRNA targeting human HCN1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

HCN1 (HCN1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pEasyG2_zeo

Plasmid

#184913

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_zeo recombines in vivo with a PCR product from pEasyG2_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pEasyG2_nat

Plasmid

#184914

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEasyG3_zeo

Plasmid

#184917

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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