We narrowed to 10,108 results for: Coli

Showing: 8041 - 8060 of 10108 results

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pET-p120RasGAP-714-1047-WT

Plasmid

#245307

Purpose

Expresses GAP domain of human p120RasGAP (residues 714-1047 wild type) in E. coli

Depositor

Titus Boggon

Article

Paul et al Methods. 2025 Dec;244:108-117. doi: 10.1

Insert

RASA1 (RASA1 Human)

Tags

6His

Expression

Bacterial

Mutation

deleted amino acids 1-713

Promoter

T7lac

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET-p120RasGAP-581-1047-WT

Plasmid

#246487

Purpose

Expresses C2 and GAP domains of human p120RasGAP (residues 581-1047 wild type) in E. coli

Depositor

Titus Boggon

Article

Paul et al Methods. 2025 Dec;244:108-117. doi: 10.1

Insert

RASA1 (RASA1 Human)

Tags

6His

Expression

Bacterial

Mutation

deleted amino acids 1-580

Promoter

T7lac

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pdnCas3-CDA1

Plasmid

#229535

Purpose

pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

Depositor

Kevin Verstrepen

Article

Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

Inserts

dnCas3
Cytidine deaminase
Uracil glycosylase inhibitor

Tags

XTEN

Expression

Bacterial and Yeast

Mutation

codon optimized for S.cerevisiae and codon optimi…

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pdnCas3-APOBEC1

Plasmid

#229537

Purpose

pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

Depositor

Kevin Verstrepen

Article

Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

Inserts

rAPOBEC1
dnCas3
Uracil glycosylase inhibitor

Tags

XTEN

Expression

Bacterial and Yeast

Mutation

codon optimized for S.cerevisiae and codon optimi…

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pJExpress401-KDAC4 CD H976Y

Plasmid

#224250

Purpose

Expresses human KDAC4 (HDAC4) catalytic domain H976Y in E. coli

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2017 Aug 29;56(34):4549-45

Insert

KDAC4 (HDAC4 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Only includes residues 648-1083 (catalytic domain…

Promoter

T5

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR

Plasmid

#169135

Purpose

E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

Depositor

Michael Nash

Article

Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

Insert

Coh7

Tags

3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…

Expression

Bacterial

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyCatcher -AVI

Plasmid

#223635

Purpose

Protein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

AVI tag and His6

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGGI_Myr-YFP-MiniTurboID

Plasmid

#222432

Purpose

Potential plasma membrane control.

Depositor

Dominique Bergmann

Article

Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

Insert

MiniTurboID (BirA mutant)

Use

Golden gate / green gate compatible cloning vector

Tags

Linker, Myr, and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pC1-lifeact-oROS-HT

Plasmid

#216420

Purpose

Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to actin filaments.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

lifeact-oROS-HT

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET151-GyrA_F164R238

Plasmid

#216230

Purpose

S. aureus USA300 GyrA mutant F164 R238 residues codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Tags

6xHis

Expression

Bacterial

Mutation

Changed arginine 238 to serine, phenylalanine 168…

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET151-GyrB_A467

Plasmid

#216234

Purpose

S. aureus USA300 GyrB mutant A467 residue codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Tags

6xHis

Expression

Bacterial

Mutation

Changed alanine 467 to valine

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET151-GyrB_T522

Plasmid

#216235

Purpose

S. aureus USA300 GyrB mutant T522 residue codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Tags

6xHis

Expression

Bacterial

Mutation

Changed threonine 522 to isoleucine

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-MBP-TEV-NAP1_delta-NDP52 (S37K/A44E)

Plasmid

#208872

Purpose

For expression in E. coli of NAP1 (delta-NDP52)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

MBP-TEV

Expression

Bacterial

Mutation

S37K/A44E (disrupts NDP52 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-MBP-TEV-NAP1_delta-TBK1 (L226Q/L233Q)

Plasmid

#208873

Purpose

For expression in E. coli of NAP1 (delta-TBK1)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

MBP-TEV

Expression

Bacterial

Mutation

L226Q/L233Q (disrupts TBK1 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV R354H SPOP 28-359

Plasmid

#197011

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Human)

Tags

6His-SUMO-TEV

Expression

Bacterial

Mutation

R354H

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot4_275-575-del377-402-GB1His6_AG

Plasmid

#148785

Purpose

E. coli of HsNot4_275-575-del307-402

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-del307-402 (CNOT4 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot4_275-575-del400-428-GB1His6_AG

Plasmid

#148787

Purpose

E. coli of HsNot4_275-575-del400-428

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-del400-428 (CNOT4 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70DAmt1-DadR/DadS-Pdadh-DadhABC(R506S, A2)

Plasmid

#191631

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain (R506S mutation) with transcriptional regulators DadR/DadS

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

Dopamine dehydroxylase (R506S mutation, inactive form)

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.0RTcgr-Pct5.1-crRNA(AarI)-RT(Δcgr12)

Plasmid

#191651

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer) cgr1/cgr2-deleting repair template, used as negative control for pXD71Cas10.4RT4

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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KTK_113

Plasmid

#180540

Purpose

Entry vector containing Signal peptide region of CsgA including SecB-N22 sequenced

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Signal peptide region of CsgA including SecB-N22 sequenced

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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