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pLKO_AU1.C.V5_mCherry-NLS

Plasmid

#178215

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.C.V5 and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.C.Ollas_mCherry-NLS

Plasmid

#178212

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.C.Ollas and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_HSV.Ollas.S_mCherry-NLS

Plasmid

#178276

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

HSV.Ollas.S and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_HA.HSV.Ollas_mCherry-NLS

Plasmid

#178259

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

HA.HSV.Ollas and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_HA.HSV.NWS_mCherry-NLS

Plasmid

#178258

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

HA.HSV.NWS and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-PLXNB2-decto

Plasmid

#86238

Purpose

Lentivirus for expression of human Plexin-B2 with deletion of ectodomain

Depositor

Roland Friedel

Article

Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

Insert

Plexin-B2 (PLXNB2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

PLXNB2 with deletion of extracellular domain

Promoter

hPGK

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-PLXNB2-mRBD

Plasmid

#86240

Purpose

Lentivirus for expression of human Plexin-B2 with mutant RBD domain

Depositor

Roland Friedel

Article

Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

Insert

Plexin-B2 (PLXNB2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

PLXNB2 with point mutations of RBD domain

Promoter

hPGK

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-PLXNB2-dVTDL

Plasmid

#86239

Purpose

Lentivirus for expression of human Plexin-B2 with deletion of C-terminal VTDL aminoacids

Depositor

Roland Friedel

Article

Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

Insert

Plexin-B2 (PLXNB2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

PLXNB2 with deletion of last 4 aminoacids

Promoter

hPGK

Available Since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAG 442TAA

Plasmid

#154779

Purpose

plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAG 277TAA, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

LaG17-SynNotch

Tags

LaG17

Expression

Mammalian

Mutation

204TAG 442TAA in LaG17-SynNotch, hybrid PylT with…

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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p3-HPRT1-S104R-PdTK-mCh

Plasmid

#107276

Purpose

HPRT1 c.312C>A Munich donor vector with unilateral microhomology

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Inserts

HPRT genomic DNA (HPRT1 Human)
puro-deltaTK; CAG::mCherry

Mutation

c.306G>T, c.312C>A

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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FRT2-H2B-EGFP-V5-2A (IG157)

Plasmid

#99623

Purpose

To clone gene of interest downstream of FRT2-H2B-EGFP-V5-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

H2B-EGFP-V5

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMyc-CMV1-huTLR4mut-C2141A

Plasmid

#53527

Purpose

expression of mutant TLR4 in mammalian cells

Depositor

Linda Yu

Article

Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

Insert

TLR4 C2141A (TLR4 Human)

Tags

Myc

Expression

Mammalian

Mutation

C2141A

Promoter

CMV

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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p3-HPRT1-S104Rf-PdTK-mCh

Plasmid

#107277

Purpose

HPRT1 c.312C>A (Munich) donor vector with bilateral microhomology

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Inserts

HPRT genomic DNA (HPRT1 Human)
puro-deltaTK; CAG::mCherry

Mutation

c.306G>T, c.312C>A

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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FRT1-Mb2YFP-2A (IG153)

Plasmid

#99619

Purpose

To clone gene of interest downstream of FRT1-Mb2YFP-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2EYFP

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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FRT2-Mb2Tomato-2A (IG154)

Plasmid

#99620

Purpose

To clone gene of interest downstream of FRT2-Mb2Tomato-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2Tomato

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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FRT3-HA-H2B-Cerulean-2A (IG158))

Plasmid

#99624

Purpose

To clone gene of interest downstream of FRT3-HA-H2B-Cerulean-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

HA-H2B-Cerulean

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMyc-CMV1-huTLR4mut-C1196T

Plasmid

#53526

Purpose

expression of mutant TLR4 in mammalian cells

Depositor

Linda Yu

Article

Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

Insert

TLR4 C1196T (TLR4 Human)

Tags

Myc

Expression

Mammalian

Mutation

C1196T

Promoter

CMV

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only

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pMyc-CMV1-huTLR4mut-A896G

Plasmid

#53525

Purpose

expression of mutant TLR4 in mammalian cells

Depositor

Linda Yu

Article

Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

Insert

TLR4 A896G (TLR4 Human)

Tags

Myc

Expression

Mammalian

Mutation

A896G

Promoter

CMV

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only

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pEf1a-Dn29-dCas9-P2A-GFP

Plasmid

#247155

Purpose

Mammalian expression of a human codon optimized wildtype Dn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Dn29-dCas9-P2A-GFP

Tags

SV40 NLS

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW122_SpG-Cas9_U6-entry

Plasmid

#244819

Purpose

Mammalian expression of SpG Cas9 with Golden Gate-compatible sgRNA spacer cassette

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpG Cas9

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Mutation

D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

CAG, U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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