We narrowed to 14,585 results for: pET

1. pET-22HT-MSI1(7-192)R53E/R59E/R61E

   Plasmid

   #60355

   Purpose

   R53E R59E R61E triple mutant of the mouse MSI1 RBD

   Depositor

   Sean Ryder

   Article

   Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

   Insert

   mouse MSI1 (7-192) (Msi1 Mouse)
   

   Use

   Tags

   His and TEV protease site

   Expression

   Bacterial

   Mutation

   R53E, R59E and R61E mutations; contains amino aci…

   Promoter

   T7

   Available since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pETDuet1_6xHis-TEV-mCherry-WIPI2d IDR (364-425aa)

   Plasmid

   #223790

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   WIPI2d IDR (WIPI2 Human)
   

   Use

   Tags

   6xHis-TEV-mCherry

   Expression

   Bacterial

   Mutation

   aa 364-425 only

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETDuet1_6xHis-TEV-mCherry-ATG13 IDR (191-517aa)

   Plasmid

   #223762

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   ATG13 IDR (ATG13 Human)
   

   Use

   Tags

   6xHis-TEV-mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETDuet1_NIX(1-182aa)-THROMBIN-GST (W36A/L39A; deltaLIR)

   Plasmid

   #223738

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   NIX (BNIP3L) soluble part (BNIP3L Human)
   

   Use

   Tags

   Thrombin cleavage-GST

   Expression

   Bacterial

   Mutation

   W36A/L39A; deletion of LIR

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETDuet1_FUNDC1(1-50aa)-THROMBIN-GST (Y18A/L21A; deltaLIR)

   Plasmid

   #223739

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   FUNDC1 soluble part, mutant (FUNDC1 Human)
   

   Use

   Tags

   Thrombin cleavage-GST

   Expression

   Bacterial

   Mutation

   Y18A/L21A; deletion of LIR

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (W276A/I279A; deltaLIR1)

   Plasmid

   #223749

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   BCL2L13 soluble part (BCL2L13 Human)
   

   Use

   Tags

   Thrombin cleavage-GST

   Expression

   Bacterial

   Mutation

   expresses aa 1-465, with W276A/I279A; deletion of…

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETDuet1_6xHis-TEV-EGFP-FIP200-CTR(1429-1591aa)

   Plasmid

   #223724

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   FIP200-CTR (RB1CC1 Human)
   

   Use

   Tags

   6xHis-TEV-EGFP

   Expression

   Bacterial

   Mutation

   aa 1429-1591 only

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28b-SUMO-FLAG-TeKaiB-1-94-Y8A-Y94A-FLAG

   Plasmid

   #229223

   Purpose

   For expression of T. elongatus KaiB protein mutant

   Depositor

   Andy LiWang

   Insert

   kaiB (kaiB T. elongatus)
   

   Use

   Tags

   FLAG and SUMO-FLAG

   Expression

   Bacterial

   Mutation

   1-94 (truncated), Y8A, Y94A

   Promoter

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAM316 pET28a-GGG-Titin[V15P]-23-K-9-C-14cyclinB-10GS-Y

   Plasmid

   #226349

   Purpose

   Expression of Titin V15P substrate with GS tail

   Depositor

   Andreas Martin

   Article

   Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

   Insert

   titin (TTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V15P

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR

    Plasmid

    #169135

    Purpose

    E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    Coh7
    

    Use

    Tags

    3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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