We narrowed to 28,941 results for: Tat

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pcDNA3.1-FLAG-SBP-STAG2 (I885fs)

Plasmid

#73966

Purpose

STAG2 cDNA (CCDS43990) I885fs

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Tags

FLAG-SBP

Expression

Mammalian

Mutation

I885fs

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FLAG-SBP-STAG2 (S202L)

Plasmid

#73972

Purpose

STAG2 cDNA (CCDS43990) S202L

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Tags

FLAG-SBP

Expression

Mammalian

Mutation

S202L

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FLAG-SBP-STAG2 (V181M)

Plasmid

#73971

Purpose

STAG2 cDNA (CCDS43990) V181M

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Tags

FLAG-SBP

Expression

Mammalian

Mutation

V181M

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only
pJK287

Plasmid

#71120

Purpose

Produces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (H6UctB) in E. coli

Depositor

T. J. Kappock

Article

Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

Insert

formyl-CoA:oxalate CoA-transferase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJK360

Plasmid

#71571

Purpose

Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala mutant (UctC-T2A)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

acetyl-CoA:oxalate CoA-transferase

Expression

Bacterial

Mutation

changes threonine-2 to alanine

Promoter

T7

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pES007 (pTet-qacR 5/pQacA-deGFP)

Plasmid

#69036

Purpose

reporter with qacR 5 on single plasmid

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Inserts

qacR (qacR Synthetic)
deGFP

Tags

His6 tag

Expression

Bacterial

Mutation

W61Y, E90Q, F102S, M116Q, Y119L, E120Q

Promoter

pQacA and pTet

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pES003 (pTet-qacR 2)

Plasmid

#69032

Purpose

qacR 2 downstream of a Tet promoter

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Insert

qacR (qacR Synthetic)

Tags

His6 tag

Expression

Bacterial

Mutation

E57Q, E58L, W61Y, E90Q, I99Q, M116Q, L119Y, E120Q…

Promoter

pTet

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pICSL11062

Plasmid

#68256

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-2 in Brassica oleracea

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter_AtU6-26 + sgRNA_BolC.GA4a-2

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pClneoEGFPC-let60 K16N

Plasmid

#66861

Purpose

Express GFP-tagged dominat negative let60, in which Lysine 16 is mutated to asparagine, in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

Insert

Let-60 (let-60 Nematode)

Tags

GFP

Expression

Mammalian

Mutation

Lysine 16 is mutated to asparagine

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pET30-SBL1(160S,HisTagDel)

Plasmid

#66880

Purpose

Expresses a version of pET30Xa-SBL1(160S) with 41 bp Histag removed

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 (S160S)

Tags

none

Expression

Bacterial

Mutation

none

Promoter

T7lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pET30-SBL1(160E,HisTagDel)

Plasmid

#66879

Purpose

Expresses a version of pET30Xa-SBL1(160E) with 41 bp Histag removed

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 (S160E)

Expression

Bacterial

Mutation

S160E

Promoter

T7lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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CFP(1-158) in pcDNAI/Amp

Plasmid

#60895

Purpose

This vector attaches CFP(1-158) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

Insert

CFP(1-158)

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pRevTre-mKLF5

Plasmid

#41744

Depositor

Jonathan Katz

Article

Yang et al Cell Cycle. 2012 Nov 1;11(21):4033-9. do

Insert

Klf5 (Klf5 Mouse)

Expression

Mammalian

Available Since

July 13, 2015

Availability

Academic Institutions and Nonprofits only
pFRT-TODestFLAGHA_RBPMS_KDR_K100A

Plasmid

#65663

Purpose

FLAG-HA expression vector with RBPMS carrying mutation K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

changed lysine 100 to alanine (KDR background)

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS_KDR_F27A

Plasmid

#65661

Purpose

FLAG-HA expression vector with RBPMS carrying mutation F27A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

changed phenylalanine 27 to alanine (KDR backgrou…

Promoter

CMV, 2x Tet

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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Danio rerio cryaa V62T

Plasmid

#47630

Purpose

Recombinant protein production

Depositor

Mason Posner

Article

Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

Insert

Alpha A-crystallin (cryaa Zebrafish)

Expression

Bacterial

Mutation

Changed Valine 62 to Threonine

Promoter

T7

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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psicheck-2-Tas2r3-mod

Plasmid

#48175

Purpose

contains Renilla Luciferase gene preceded by a mutated (ATG --> ACG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

TAS2R3 taste 2 receptor member 3 (TAS2R3 Human)

Use

Luciferase

Mutation

T/C MUTATION IN ATG OF UORF

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
Danio rerio cryaa T147V

Plasmid

#47628

Purpose

Recombinant protein production

Depositor

Mason Posner

Article

Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

Insert

Alpha A-crystallin (cryaa Zebrafish)

Expression

Bacterial

Mutation

Changed Theronine 147 to Valine

Promoter

T7

Available Since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

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Danio rerio cryaa C143S

Plasmid

#47619

Purpose

Recombinant protein production

Depositor

Mason Posner

Article

Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

Insert

Alpha A-crystallin (cryaa Zebrafish)

Expression

Bacterial

Mutation

Cysteine 143 to Serine

Promoter

T7

Available Since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

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psicheck-2-Phb mod

Plasmid

#48163

Purpose

contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading from element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of Phb (Phb Mouse)

Use

Luciferase

Mutation

A/T mutation in the ATG of the uORF

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only