We narrowed to 9,383 results for: BLI

1. pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56

   Plasmid

   #208645

   Purpose

   Remove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.

   Depositor

   Quanfu Ma

   Insert

   Twin Strep tag with RPL3 genome HomoArm (RPL3 Synthetic, Human)
   

   Use

   CRISPR; Ssdna donnor preparation for knock-in of…

   Tags

   Twin Strep

   Mutation

   Two SNP (rs749555789, rs760194863) were introduce…

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWR54

   Plasmid

   #203330

   Purpose

   episomal bioluminescent reporter plasmid for S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
   coHPH
   ACT1 terminator (S. stipitis)
   CCW12 promoter (S. stipitis)
   coCBG
   ADH2 terminator (S. stipitis)
   ARS from S. stipitis chromosome 1
   

   Expression

   Yeast

   Available Since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWR24

   Plasmid

   #202771

   Purpose

   episomal bioluminescent reporter plasmid for S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
   coNAT
   ACT1 terminator (S. stipitis)
   TDH3 promoter (S. stipitis)
   coCBG
   ADH2 terminator (S. stipitis)
   ARS from S. stipitis chromosome 1
   

   Expression

   Yeast

   Available Since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWR16

   Plasmid

   #202711

   Purpose

   integrating bioluminescent reporter plasmid for S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
   coHPH
   ACT1 terminator (S. stipitis)
   TDH3 promoter (S. stipitis)
   coCBG
   ADH2 terminator (S. stipitis)
   URA3 and flanking sequence
   

   Expression

   Yeast

   Available Since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX458_3XHA_dFnCas9_KRAB_T2A_eGFP

   Plasmid

   #200389

   Purpose

   3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression

   Depositor

   Debojyoti Chakraborty

   Insert

   PX458_3XHA_dFnCas9_KRAB_eGFP
   

   Use

   CRISPR

   Tags

   3xHA-NLS and SV40NLS-KRAB-T2A-EGFP

   Expression

   Mammalian

   Promoter

   CBH

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-SFFV-Llgl1-WPRE-UbC-Emerald

   Plasmid

   #203805

   Purpose

   Lentiviral vector plasmid expressing mouse Llgl1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Inserts

   Llgl1 (Llgl1 Mouse)
   Emerald
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-6p-1_GST-DmKHC[1-421]-1xGFP

   Plasmid

   #196973

   Purpose

   Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and cleavable N-terminal GST tag

   Depositor

   Lukas Kapitein

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Tags

   GFP and GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-6p-1_GST-SNAP-DmKHC[1-421]

   Plasmid

   #196976

   Purpose

   Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with N-terminal SNAP tag and cleavable N-terminal GST

   Depositor

   Lukas Kapitein

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Tags

   GST and SNAP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-SU(Z)12(624-900)-H6

   Plasmid

   #183772

   Purpose

   Express of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.

   Depositor

   Peter Harte

   Article

   Tie et al Mol Cell Biol. 2003 May;23(9):3352-62. d

   Insert

   SU(Z)12 residues 624-900 (Su(z)12 Fly)
   

   Tags

   His tag at the C-terminus

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT5T_Rev7_1-211_SA112_KVK_Rev3_1873-1898

    Plasmid

    #105635

    Purpose

    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1873-1898) from bicistronic plasmid

    Depositor

    Thanos Halazonetis

    Inserts

    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    

    Expression

    Bacterial

    Mutation

    Mutations: F11S, G12A, V132K, C133V, A135K

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT5T_Rev7_1-211_SA112_KVK_Rev3_1989-2014

    Plasmid

    #105637

    Purpose

    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1989-2014) from bicistronic plasmid

    Depositor

    Thanos Halazonetis

    Inserts

    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    

    Expression

    Bacterial

    Mutation

    Mutations: F11S, G12A, V132K, C133V, A135K

    Promoter

    T7

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2+WBP2NL-HA

    Plasmid

    #174791

    Purpose

    Xenopus laevis WBP2NL with 3'HA tag

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    WBP2 N-terminal like (wbp2nl.L Frog)
    

    Tags

    HA

    Expression

    Bacterial

    Mutation

    aa#165, Y to H; aa# 287, D to H

    Promoter

    SP6

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR4 CDS A. thaliana PARP1

    Plasmid

    #175818

    Purpose

    pENTR4 plasmid with CDS of Arabidopsis thaliana POLY(ADP-RIBOSE) POLYMERASE 1 (PARP1, AT2G31320.1) without stop codon for C-terminal epitope tags

    Depositor

    Lennart Wirthmueller

    Insert

    PARP1 (PARP1 Mustard Weed)
    

    Use

    Pentr plasmid for gateway cloning

    Tags

    none

    Mutation

    one silent mutation, annotated in GenBank file

    Promoter

    none

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2+-wbp2nl

    Plasmid

    #172463

    Purpose

    expresses wild-type protein when injected into embryos

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nl (wbp2nl.L Frog)
    

    Use

    Xenopus expression

    Tags

    none

    Mutation

    none

    Promoter

    SP6

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pB6-Id1-loxP-AU1-rox-FNF-loxP-drePBD*-rox

    Plasmid

    #168770

    Purpose

    B6J Id1 AU1 tag and LSL-TagRFP-T-drePBD* targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    Id1 (Id1 Mouse)
    

    Use

    Cre/Lox and Mouse Targeting; Dre / rox

    Mutation

    See Depositor Comments Below

    Available Since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pB6-Cdk6-T2A-TagRFPT-dre-PBD*-FNF-TK

    Plasmid

    #141452

    Purpose

    B6J Cdk6-T2A-TagRFPT-dre-PBD* allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    Cdk6 (Cdk6 Mouse)
    

    Use

    Mouse Targeting; Dre / rox

    Tags

    C-terminal fusion of T2A-TagRFPT-dre-PBD*

    Expression

    Mammalian

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. psiCHECK2 3'UTR GAC

    Plasmid

    #110401

    Purpose

    Luminescence-based glutaminase GAC isoform 3'UTR-mediated RNA stability reporter

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GLS glutaminase (GLS Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC19 KGA HA5'-mKO2-3'

    Plasmid

    #110406

    Purpose

    Homology donor for glutaminase KGA isoform knock-in, mKO2

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GLS glutaminase (GLS Human)
    

    Use

    CRISPR

    Promoter

    none

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFA-DAB1

    Plasmid

    #162691

    Purpose

    Expresses DabAB1 inorganic carbon transport complex

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    DabAB1 inorganic carbon transport complex
    

    Expression

    Bacterial

    Promoter

    PLtet0-1 promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. mPLD2-PX (62-192)

    Plasmid

    #119128

    Purpose

    Bacterial expression of human phox homology (PX) domain, mPLD2-PX (62-192)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    mPLD2-PX (62-192)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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