We narrowed to 40,825 results for: Ina;

1. pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2

   Plasmid

   #189632

   Purpose

   Lentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs libraries

   Depositor

   Manuel Kaulich

   Article

   Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

   Insert

   h7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. SUMO-Twinkle 43-372

   Plasmid

   #205054

   Purpose

   TWNK protein expression

   Depositor

   Smita Patel

   Article

   Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

   Insert

   TWNK (TWNK Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Lacks Mitochondrial localization signal and C ter…

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNK005C_BattB_mkozak_STIM1_IRES_mCherry-H2A-P2A-PuroR

   Plasmid

   #200639

   Purpose

   Low STIM1 abundance recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

   Insert

   STIM1 (STIM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5 PSK (745-1235)

   Plasmid

   #197863

   Purpose

   Expression of C-terminal PSK1 / TAOK2 (aa745-1235) in mammalian cells (microtubule-binding domain)

   Depositor

   Jonathan Morris

   Article

   Mitsopoulos et al J Biol Chem. 2003 May 16;278(20):18085-9

   Insert

   PSK1 (745-1235) (TAOK2 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   The N terminus of PSK1/TAOK2 has been deleted

   Promoter

   SV40

   Available since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. CB6-GFP-TAOK1 (K57A)

   Plasmid

   #197109

   Purpose

   Expression of kinase-deficient GFP-tagged TAOK1 (K57A) / PSK2 (K57A) in mammalian cells

   Depositor

   Jonathan Morris

   Article

   Garg et al J Cell Sci. 2020 Mar 16;133(6):jcs235895

   Insert

   TAOK1 (K57A) (TAOK1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed K 57 to A

   Promoter

   CMV

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEE055

   Plasmid

   #176812

   Purpose

   Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

   Insert

   606
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. W118-1_hSTK16-Flag

   Plasmid

   #180403

   Purpose

   lentiviral transduction of human STK16 (with a C-terminal Flag tag) gene

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   STK16 (STK16 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. W118-1_Flag-hSRPK3

   Plasmid

   #180404

   Purpose

   lentiviral transduction of human SRPK3 (with an N-terminal Flag tag) gene

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   SRPK3 (SRPK3 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAT15541_dCBE-SuperFi-Cas9

   Plasmid

   #184371

   Purpose

   Mammalian expression of nuclease inactive SuperFi-Cas9 CBE base editor

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   dCBE-SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   3X FLAG, SV40 NLS, and UGI

   Expression

   Mammalian

   Mutation

   D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

   Promoter

   EF-1α core promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAT15543_dABE-SuperFi-Cas9

    Plasmid

    #184373

    Purpose

    Mammalian expression of nuclease inactive SuperFi-Cas9 ABE7 base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    dABE-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    FLAG, SV40 NLS, and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

    Promoter

    EF-1α core promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAT15545_dABE8e-SuperFi-Cas9

    Plasmid

    #184375

    Purpose

    Mammalian expression of nuclease inactive SuperFi-Cas9 ABE8e base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    dABE8e-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

    Promoter

    EF-1α core promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tol2-LyzC-Rac-DN(guide resistant)-2a-mcherry-U6ac-Rac guides

    Plasmid

    #168243

    Purpose

    "Dominant negative control of the assay to rescue Rac expression in neutrophil specific knockout"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    Rac(guide resistant)-DN
    

    Use

    Zebrafish expression

    Tags

    mcherry

    Expression

    Mutation

    Promoter

    LyzC

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGem-LP1-loxP-bar-lox2272-71

    Plasmid

    #168785

    Purpose

    Vector to create landing pad 1 (LP1) by homologous recombination in Aspergillus nidulans ΔSt site. The strain A. nidulans LP1 is used for recombinase mediated chromosomal integration.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102

    Inserts

    bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    loxP
    lox2272-71
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGem-LP2-loxP-bar-lox2272-71

    Plasmid

    #168786

    Purpose

    Vector to create landing pad 2 (LP2) by homologous recombination in Aspergillus nidulans IS1 site. The strain A. nidulans LP2 is used for recombinase mediated chromosomal integration.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102

    Inserts

    bar
    IS1 Homology arm 1
    IS1 Homology arm 2
    loxP
    lox2272-71
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. mILK-R349A-EFGP

    Plasmid

    #176898

    Purpose

    mouse ILK with R349A point mutation cloned into pEGFP-N1

    Depositor

    Sara Wickström

    Article

    Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

    Insert

    Integrin-linked kinase (Ilk Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Arginine 349 to Alanine

    Promoter

    CNV

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. mILK-R255A-EFGP

    Plasmid

    #176897

    Purpose

    mouse ILK with R255A point mutation cloned into pEGFP-N1

    Depositor

    Sara Wickström

    Article

    Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

    Insert

    Integrin-linked kinase (Ilk Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Arginine 255 to Alanine

    Promoter

    CNV

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-3xFLAG-Puro PAK5 K478R/479R S364L

    Plasmid

    #167199

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R/479R S364L; PAK7 Kinase dead & S364L double mutant

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R/479R S364L
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-3xFLAG-Puro PAK5 K478R/479R D421N

    Plasmid

    #167200

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R/479R D421N; PAK7 Kinase dead & D421N double mutant

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R/479R D421N
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-3xFLAG-Puro PAK5 K478R/479R E294K

    Plasmid

    #167198

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E294K; PAK7 Kinase dead & E294K double mutant

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R/479R E294K
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-3xFLAG-Puro PAK5 K478R/479R M173I

    Plasmid

    #167197

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R/479R M173I; PAK7 Kinase dead & M173I double mutant

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R/479R M173I
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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