We narrowed to 9,182 results for: Pol

1. pcDNA5 FRT/TO Plk4 KD-mCherry

   Plasmid

   #80270

   Purpose

   mammalian expression plasmid for kinase dead Plk4

   Depositor

   Andrew Holland

   Article

   Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

   Insert

   Plk4 (PLK4 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   D154A kinase dead

   Promoter

   CMV

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE80L TriCatcher-RGDSP

   Plasmid

   #112631

   Purpose

   Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site and a C-terminal SnoopCatcher

   Depositor

   Mark Howarth

   Article

   Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

   Insert

   TriCatcher-RGDSP
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   central RGDSP integrin-binding sequence

   Promoter

   T5

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. MBP-3C-hNorrin(33-133)-monomeric(C93A/C95A/C131A)-1D4_pAcGP67a

   Plasmid

   #216385

   Purpose

   Baculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218) (monomeric mutant C93A/C95A/C131A)

   Depositor

   William Weis

   Article

   Bruguera et al bioRxiv 2024.02.03.578714

   Insert

   Norrin (NDP Human)
   

   Use

   Baculovirus

   Tags

   GP64 signal sequence-MBP-3C and Rho1D4

   Expression

   Insect

   Mutation

   C93A/C95A/C131A

   Promoter

   Polyhedrin

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSBL_DK187

   Plasmid

   #156423

   Purpose

   Insect cell expression of 3a-sfGFP, needs to be introduced into baculoviral bacmid

   Depositor

   Stephen Brohawn

   Article

   Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

   Insert

   SARS-CoV-2 3a protein
   

   Tags

   sfGFP-His

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET29b-dspB(E184Q)-6xHis

   Plasmid

   #175802

   Purpose

   Plasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

   Insert

   Dispersin B
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   E184Q

   Promoter

   T7

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDB036d

   Plasmid

   #99216

   Purpose

   Accessory plasmid for PACE of MjTyrRS variants; negative selection

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)
   

   Expression

   Bacterial

   Mutation

   amber codons at positions 12 and 203 of T7 RNA po…

   Promoter

   Ppsp, PproK, and PproA

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRN3P_T3_BE3_IVT

   Plasmid

   #177015

   Purpose

   Plasmid to be used as DNA template for in-vitro RNA transcription of the BE3 base editor (C to T) by T3 RNA polymerase

   Depositor

   Timo Otonkoski

   Article

   Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

   Insert

   BE3
   

   Use

   CRISPR; Vector for in-vitro transcription

   Promoter

   T3 promoter

   Available Since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS4

   Plasmid

   #55751

   Purpose

   Produces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

   Depositor

   Matthew Bennett

   Article

   Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

   Insert

   KanR
   

   Expression

   Bacterial

   Mutation

   Residues 1-59 of KanR

   Promoter

   T7-lac

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDB036a

   Plasmid

   #99215

   Purpose

   Accessory plasmid for PACE of MjTyrRS variants; negative selection

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproD [SD4] T7RNAP(S12*,S203*)
   

   Expression

   Bacterial

   Mutation

   amber codons at positions 12 and 203 of T7 RNA po…

   Promoter

   Ppsp, PproK and PproD

   Available Since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOCC114

    Plasmid

    #118904

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase site

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-TEV-GG-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with TEV protease, two glycines

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pV2A-T

    Plasmid

    #153022

    Purpose

    Single-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoter

    Depositor

    Vito Valiante

    Article

    Hoefgen et al Metab Eng. 2018 Jul;48:44-51. doi: 10.10

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Oct. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. 3924-55

    Plasmid

    #176499

    Purpose

    pTRE-EGFP-T2A-TFEB(S3E)-polyA

    Depositor

    Albert La Spada

    Article

    Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

    Insert

    EGFP-T2A-TFEB(human; full-length; S3E mutation)-
    

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3923-51

    Plasmid

    #176498

    Purpose

    pTRE-EGFP-T2A-TFEB(S3A)-polyA

    Depositor

    Albert La Spada

    Article

    Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

    Insert

    EGFP-T2A-TFEB(human; full-length; S3A mutation)
    

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCLC-mEos2

    Plasmid

    #38010

    Depositor

    Xiaowei Zhuang

    Article

    Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Tags

    mEos2

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Sept. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET15b+PCNA1 P186C

    Plasmid

    #66173

    Purpose

    Expresses the P186C mutant of Sulfolobus solfataricus PCNA1

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 1
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    P186C

    Promoter

    T7 promoter

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOTF3-AK (clone3)

    Plasmid

    #21165

    Depositor

    James Thomson

    Article

    Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

    Insert

    POUF5F1 (genomic DNA) (POU5F1 Human)
    

    Use

    Cre/Lox; Homologous recombination

    Mutation

    NeoR, EGFP, and SV40 polyA are between the two ge…

    Available Since

    June 25, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGBW-m4092686

    Plasmid

    #153895

    Purpose

    Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Bacterial

    Mutation

    Nearly native encoding;inactivating mutations in …

    Promoter

    Promoter | t7 consensus from NEB, G is +1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pRJPaph-eBFP2

    Plasmid

    #67030

    Purpose

    Plasmid for Paph-eBFP2 integration downstream of B. diazoefficiens (B. japonicum) scoI

    Depositor

    Julia Vorholt

    Article

    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

    Insert

    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    

    Use

    Conjugative bacterial vector

    Available Since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. Hy_pMT eGFP-RVFV NSs HhRz

    Plasmid

    #91801

    Purpose

    Expresses a non-polyadenylated, translatable EGFP mRNA in Drosophila

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Mol Cell. 2017 Nov 9. pii: S1097-2765(17

    Insert

    EGFP
    

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET15b+PCNA1 WT

    Plasmid

    #66139

    Purpose

    Expresses the wild type of Sulfolobus solfataricus PCNA1

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 1
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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