We narrowed to 31,565 results for: REP

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Luc-Nanog-3'UTRmut

Plasmid

#63894

Purpose

Luciferase reporter of mouse Nanog 3'UTR with the miR-34 target site mutated

Depositor

Lin He

Article

Choi et al Nat Cell Biol. 2011 Oct 23;13(11):1353-6

Insert

Nanog homeobox (Nanog Mouse)

Expression

Mammalian

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-mETV3-HA

Plasmid

#33375

Depositor

Peter Murray

Article

El Kasmi et al J Immunol. 2007 Dec 1;179(11):7215-9.

Insert

Etv3 (Etv3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only
pFP121

Plasmid

#21670

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been modified, the E…

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pHAGE2-TetO-mCherry

Plasmid

#216174

Purpose

Generate lentiviruses to act as a positive transduction control for pHAGE2-TetO vectors in pluripotency reprogramming

Depositor

Ralf Jauch

Article

Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

Insert

mCherry

Use

Lentiviral

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-hfYFP-TEV-SAV

Plasmid

#191197

Purpose

Fusion of hfYFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & Streptavidin fusion

Tags

6xHis and Streptavidin core domain, amino acids 1…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-LSSmGFP-TEV-SAV

Plasmid

#191200

Purpose

Fusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP & Streptavidin fusion

Tags

6xHis and Streptavidin core domain, amino acids 1…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Mito(OM)-deltaCKAR

Plasmid

#222431

Purpose

FRET-based reporter for monitoring deltaPKC activity at the mitochondrial outer membrane in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Mitochondrial outer membrane delta-selective C kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJB65

Plasmid

#226319

Purpose

E. coli protein expression for protein purification

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

6xHis- (VTG to AAA MR1-6)-strep

Tags

6xHis and Strep

Expression

Bacterial

Mutation

N-terminal domain deleted, C-terminal domain dele…

Promoter

T7

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR092-L31

Plasmid

#185045

Purpose

J23108(spel), pHP14 stability hairpin, strong RBS, L31 coding region, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR112

Plasmid

#185048

Purpose

J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region K2A mutation, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31p

Expression

Bacterial

Mutation

K2A amino acid mutation

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR113

Plasmid

#185049

Purpose

J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region H6A mutation, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31p

Expression

Bacterial

Mutation

H6A amino acid mutaitons

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR114

Plasmid

#185050

Purpose

J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region E8A mutation, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31p

Expression

Bacterial

Mutation

E8A amino acid mutation

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR120

Plasmid

#185041

Purpose

J23108 promoter, l31 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Expression

Bacterial

Mutation

Only first 90 nt of rpmE coding present

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR089-L36p

Plasmid

#185042

Purpose

J23108(spel), pHP14 stability hairpin, strong RBS, L36p coding region, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L36p

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR090-L36

Plasmid

#185043

Purpose

J23108(spel), pHP14 stability hairpin, strong RBS, L36 coding region, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L36

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR091-L31p

Plasmid

#185044

Purpose

J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31p

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pTcI38-PRM-wasabi

Plasmid

#195554

Purpose

Temperature-dependent expression of mWasabi reporter with transcription activation by TcI38

Depositor

Mikhail Shapiro

Article

Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

Inserts

mWasabi
TcI38

Tags

6x His and AANDENYADAS degradation tag

Expression

Bacterial

Mutation

A67T, M1V, L65S, K68R, F115L, D126G, D188G

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pS44i8GH-2

Plasmid

#207526

Purpose

Conditionally-replicating in Pseudomonas vector, high-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; SmR/SpR

Depositor

Pablo Ivan Nikel

Article

Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

Insert

oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP

Use

CRISPR; Pseudomonas vector

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pYUBcLIC-GFP

Plasmid

#183509

Purpose

The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in mycobacteria.

Depositor

Martin Högbom

Article

Grave et al Protein Expr Purif. 2022 Oct;198:106132.

Type

Empty backbone

Tags

10x His-tag and folding reporter GFP

Expression

Bacterial

Promoter

T7 lac

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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LLP729_L2_pV01_0I_FRT-OCS-rLUC_TCTP-fLUC

Plasmid

#192372

Purpose

To test for the repressive potential of the OCS with the FRT sites around it. Then used as a control to represent the off state of many cicuits.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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