We narrowed to 11,847 results for: NSI

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pTRIP-SFFV-mtagBFP-2A STINGHAQ

Plasmid

#102587

Purpose

Lentivector to express STING HAQ and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

R71H-G230A-R293Q (HAQ)

Promoter

SFFV

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-NMHC-IIA-S1916A

Plasmid

#101040

Purpose

Expresses GFP-MYH9 construct (human myosin IIA) carrying mutation of serine 1916 to alanine, driven by CMV promoter. Thus inhibits PKC phosphorylation of the tail. Also carries S1915A mutation.

Depositor

Tom Egelhoff

Article

Rai et al J Biol Chem. 2017 Feb 24;292(8):3099-311

Insert

Non-muscle myosin IIA heavy chain (MYH9 Human)

Tags

GFP

Expression

Mammalian

Mutation

S1916A, S1917A

Promoter

CMV

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-oScarlet

Plasmid

#137137

Purpose

Intersectional viral expression of oScarlet in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-oScarlet

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E95D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-GCaMP6M

Plasmid

#137121

Purpose

Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-GCaMP6M

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

Removed RSET tag

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING-354STOP

Plasmid

#102589

Purpose

Lentivector to express STING delta 354 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

delta 354

Promoter

SFFV

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-BFP

Plasmid

#137129

Purpose

Intersectional viral expression of BFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-BFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF655

Plasmid

#88578

Purpose

Donor Vector containing ZNF655 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF655 (ZNF655 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-BFP

Plasmid

#137131

Purpose

Intersectional viral expression of BFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-BFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-sRGECO

Plasmid

#137127

Purpose

Intersectional viral expression of sRGECO in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-sRGECO

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E217D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLJM1-Lyso-FLAG-GFP-Sac1-Cat-CS

Plasmid

#134653

Purpose

lysosome-targeted Sac1 catalytic domain C389S

Depositor

Roberto Zoncu

Article

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

Sac1 catalytic domain (77-520 aa) CS mutant (SACM1L Human)

Use

Lentiviral

Tags

FLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…

Expression

Mammalian

Mutation

C389S and contains only amino acids 77-520 (Pleas…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 S_nostop

Plasmid

#152988

Purpose

Gateway-compatible Entry vector, with insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-BRD9-PGK-Puro-IRES-GFP

Plasmid

#75114

Purpose

Retroviral expression plasmid encoding wild type human BRD9

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-PE2-p53DD

Plasmid

#196582

Purpose

Mammalian expression of SpCas9 prime editor 2 with P2A-p53DD

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

PE2-P2A-mp53DD

Expression

Mammalian

Mutation

Δ40-903

Promoter

CMV

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-N2-6MYC-hMID2

Plasmid

#51035

Purpose

mammalian expression of myc-tagged human MID2

Depositor

Timothy Cox

Article

Short et al BMC Cell Biol. 2002;3:1. Epub 2002 Jan 4

Insert

MID2 (MID2 Human)

Tags

MYC

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF736

Plasmid

#101602

Purpose

Donor Vector containing ZNF736 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF736 (ZNF736 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2V678F-VSV

Plasmid

#139349

Purpose

Expression of a gain of function TYK2-V678F protein (correspoding to JAK2-V617F)

Depositor

Sandra Pellegrini

Article

Gakovic et al J Biol Chem. 2008 Jul 4;283(27):18522-9.

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Tags

VSV

Expression

Mammalian

Mutation

V362F-V678F

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING-V155M S366A

Plasmid

#102593

Purpose

Lentivector to express STING V155M S366A and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

V155M S366A

Promoter

SFFV

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13(+7A)-IDR-mCherry-CRY2-NLS

Plasmid

#145261

Purpose

Mammalian Expression of Nuclear Opto HOXD13(+7A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(+7A)-IDR (HOXD13 Human)

Expression

Mammalian

Mutation

A-repeat expanded

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-(BsaI)_CBh-Cas9-T2A-mCherry

Plasmid

#135012

Purpose

Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide

Depositor

Yosef Shaul

Article

Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

Insert

humanized S. pyogenes Cas9

Tags

3x Flag, 3xFLAG (N terminal on insert), NLS, and …

Expression

Mammalian

Promoter

CBh

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SARS2-S-D614 (NC-flag)

Plasmid

#156418

Purpose

Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

Depositor

Hyeryun Choe, Michael Farzan

Article

Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

Insert

SARS-CoV-2 S protein (S SARS CoV2)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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