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We narrowed to 10,347 results for: plasmids 101

Showing: 8141 - 8160 of 10347 results

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pAAV-UBC-S1-GCaMP6f

Plasmid

#160722

Purpose

Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.

Depositor

Edward Boyden

Article

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

S1-GCaMP6f

Use

AAV

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Human ubiquitin C (UBC) promoter

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pXD023 - pLKO-EF1a-Puro-P2A-FLuc-WPRE

Plasmid

#192203

Purpose

Lenti-PL(Puro-Luciferase)

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Lenti-PL(Puro-Luciferase)

Use

Lentiviral; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3 1 kb prom. + Enhancer CD274

Plasmid

#107005

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

N.B. There are two published SNPs in this region …

Promoter

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pOTTC589 - pAAV c-fos Nuc-eYFP

Plasmid

#59133

Purpose

An AAV vector that expresses nuclear localized eYFP under control of the c-fos promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Nuclear-localized Enhanced Yellow Fluorescent Protein

Use

AAV

Tags

3xNLS

Expression

Mammalian

Mutation

Promoter

mFos

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-nSpCas9-NoABE

Plasmid

#209043

Purpose

Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase)

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

nSpCas9

Use

CRISPR and Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

D10A

Promoter

Tight TRE promoter

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC337 - pAAV EF1a DIO mCherry

Plasmid

#47636

Purpose

An AAV vector that expresses mCherry in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

mCherry

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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MYC-PGK-blast

Plasmid

#190618

Purpose

Lentiviral expression plasmid of human MYC, blast selection

Depositor

Junwei Shi

Article

Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1

Insert

MYC (MYC Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3'UTR Final

Plasmid

#107009

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro-2xMyc-Rac1-WT

Plasmid

#128580

Purpose

For mammalian cell expression of WT murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

Depositor

Julian Downward

Article

Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

Insert

Rac1 (Rac1 Mouse)

Use

Retroviral

Tags

2xMyc epitope

Expression

Mammalian

Mutation

Promoter

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC364 - pAAV CaMKIIa iRFP

Plasmid

#47903

Purpose

An AAV vector that expresses iRFP under the CaM Kinase IIa promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Infrared fluorescent protein

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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AAV pTRE-FLEX-EGFP-WPRE-bGHpA

Plasmid

#65449

Purpose

Can be used to generate AAV virus that will express EGFP from the tetO promoter in a Cre-dependent manner

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

tetO

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pscALPSblasti-TMPRSS2 Blasti

Plasmid

#158088

Purpose

Expresses TMPRSS2 in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLY099-RetroEFS-BCMABBz-PRODH2-WPRE

Plasmid

#192200

Purpose

Retro-BCMACAR-PRODH2

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Retro-BCMACAR-PRODH2 (TNFRSF17 Synthetic)

Use

Retroviral; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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RPS18_pLX307

Plasmid

#98370

Purpose

Lentiviral expression of RPS18

Depositor

Sefi Rosenbluh, William Hahn

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

RPS18 (RPS18 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_PGK-hACE2

Plasmid

#161612

Purpose

Expression of human ACE2 in mammalian cells

Depositor

Brad Rosenberg

Article

Daniloski et al Cell. 2020 Oct 24. pii: S0092-8674(20)31

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRE

Plasmid

#85453

Purpose

Lentiviral CRISPR system backbone bearing BsmBI site for S. pyogenes new guide RNAs and TdTomato.

Depositor

Aviv Regev

Article

Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

Insert

U6-sgRNA-Backbone-UP-TdTomato

Use

CRISPR and Lentiviral

Tags

NLS

Expression

Mammalian

Mutation

deletion of AA123-365 in tdTomato (please see dep…

Promoter

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pRRL_SRSF2_P95L_mCherry

Plasmid

#84022

Purpose

expression of SRSF2 mutant in mammalian cells

Depositor

Robert Bradley

Article

Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

Insert

SRSF2 (SRSF2 Human)

Use

Lentiviral

Tags

2TA-mCherry and Flag

Expression

Mammalian

Mutation

P95L

Promoter

PGK

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only
pRRL_SRSF2_P95R_mCherry

Plasmid

#84021

Purpose

expression of SRSF2 mutant in mammalian cells

Depositor

Robert Bradley

Article

Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

Insert

SRSF2 (SRSF2 Human)

Use

Lentiviral

Tags

2TA-mCherry and Flag

Expression

Mammalian

Mutation

P95R

Promoter

PGK

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-VTKD4-ChR2-YFP

Plasmid

#178721

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of ChR2-YFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

ChR2-YFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-STAT4-P2A-mCherry

Plasmid

#203853

Purpose

Tet-inducible lentiviral plasmid expressing STAT4-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

STAT4 (STAT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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