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p2T-TetON-Hygro-Arhgef11-Cry2-mCh

Plasmid

#240798

Purpose

Dox-inducible expression of Arhgef11-Cry2-mCherry

Depositor

Hans Clevers

Article

Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.

Insert

Arhgef11-Cry2-mCherry

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Promoter

TRE

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pPBpuro-ERK-KTR-mCherry

Plasmid

#214829

Purpose

Encoding a fluorescent reporter for ERK activity

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

ERK KTR-mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CAG promoter

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CA-FLEX-dtA

Plasmid

#232236

Purpose

Expresses diphtheria toxin A subunit in Cre-positive cells.

Depositor

Naoshige Uchida

Article

Tsutsui-Kimura et al Nat Neurosci. 2025 Apr;28(4):795-810. do

Insert

Diphtheria toxin A subunit

Use

AAV

Expression

Mammalian

Promoter

CA

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pColdI-GST-mGTSF1l

Plasmid

#214817

Purpose

Bacterial expression of GST-tagged mouse GTSF1l

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

GTSF1l (Gtsf1l Mouse)

Tags

6x His and GST

Expression

Bacterial

Promoter

CspA

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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B2396_BTB-EGFP

Plasmid

#215099

Purpose

Expresses BCL6 BTB(1-129)-EGFP in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

BTB-EGFP (BCL6 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-YTHDF2

Plasmid

#204406

Purpose

E. coli expression of full-length YTHDF2 with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

YTHDF2 (YTHDF2 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4_AG

Plasmid

#148763

Purpose

Mammalian Expression of HsNot4

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4 (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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SIV URA PCP-NLS-GFPEnvy

Plasmid

#189941

Purpose

Single-integration vector for inserting PCP-NLS-GFPEnvy at the URA3 locus in Saccharomyces cerevisiae

Depositor

Tineke Lenstra

Article

Brouwer et al Nat Struct Mol Biol. 2023 May;30(5):692-

Insert

PCP-NLS-GFPEnvy

Expression

Mammalian

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pETM41_KPNB1_HEAT

Plasmid

#178494

Purpose

Bacterial expression of human domain with His-tag and MBP tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

KPNB1_HEAT (KPNB1 Synthetic, Human)

Tags

His-MBP

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-hPPP3R1

Plasmid

#179136

Purpose

Expression vector of human protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1), CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1 Human)

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCFP-LacI-HOXD13wt-IDR

Plasmid

#145255

Purpose

Expresses fusion of ECFP-LacI and HOXD13wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13wt-IDR (HOXD13 Human)

Expression

Mammalian

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pDD373

Plasmid

#137021

Purpose

SapTrap GFP-GLO donor

Depositor

Daniel Dickinson, Bob Goldstein

Insert

C. elegans GLO-optimized GFP

Use

CRISPR; Saptrap donor for cloning repair templat…

Tags

C. elegans GLO-optimized GFP

Expression

Worm

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Rap1b S179AS180A

Plasmid

#118327

Purpose

Expression of bovine Rap1b

Depositor

Philip Stork

Article

Takahashi et al J Biol Chem. 2013 Sep 27;288(39):27712-2

Insert

Rap1b S179AS180A (RAP1B Bovine)

Tags

GFP

Expression

Mammalian

Mutation

S179AS180A

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pGE-attBCC-FRT-mW (pTL788)

Plasmid

#115159

Purpose

Integration vector for phiC31-mediated transgenesis using a genomic attP-CC site. Contains an attB-CC site, the visible GMR-white flanked by a FRT site for FLP-mediated removal.

Depositor

Tobias Langenhan

Article

Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

Insert

attB-CC FRT

Expression

Insect

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-RFP-miR-223

Plasmid

#97148

Purpose

miR-223 expression

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

miR-223

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pDD398

Plasmid

#91832

Purpose

AID NT-tag linker donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Auxin-inducible degron (AID)

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDD397

Plasmid

#91822

Purpose

AID FP-slot donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Auxin-inducible degron (AID)

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSLIK 3XFLAG-MKK4-EE-NLS hygro

Plasmid

#87779

Purpose

Inducible expression of constitutively active mutant MKK4 with a C-terminal NLS sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK4

Use

Lentiviral

Tags

3xFLAG and NLS

Expression

Mammalian

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only
RCAS-sgATG7

Plasmid

#75228

Purpose

An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh

Depositor

Noor Gammoh

Article

Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi:

Insert

Autophagy Related 7 (Atg7 Mouse)

Use

Retroviral

Promoter

U6

Available Since

June 2, 2016

Availability

Academic Institutions and Nonprofits only
pRF-AmeR

Plasmid

#49356

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

AmeR repressor

Use

Synthetic Biology

Expression

Bacterial

Mutation

D120F, R121Q, G123H, Y124T, E125G, M126F, W127YS

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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