We narrowed to 32,144 results for: uros

Showing: 8161 - 8180 of 32144 results

Results per page:

Sort results by:

pX68

Plasmid

#46857

Purpose

OP Hupki-puro2A-A138V plasmid to indroduce mutant p53 to PLF ESC or MEFs by IMCE

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB-Puro-2A and Hupki p53 A138V (TP53 Mouse, Human)

Expression

Mammalian

Mutation

p53 A138V

Available Since

Aug. 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pXL003-ishRNA-beta-catenin-2

Plasmid

#36299

Depositor

Sean Palecek

Article

Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

Insert

shRNA of beta-catenin-2 (CTNNB1 Human)

Use

Lentiviral and RNAi

Tags

IRES-RFP-Puro

Expression

Mammalian

Promoter

TetO-H1

Available Since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only
TBK1 gRNA (BRDN0001148607)

Plasmid

#76363

Purpose

3rd generation lentiviral gRNA plasmid targeting human TBK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TBK1 (TBK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-CMV-GCaMP6-210

Plasmid

#91778

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

Douglas Kim, GENIE Project

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP6-210

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p323.hSyn.Halo-mFMRP.Wpre

Plasmid

#185909

Purpose

Expresses FMRP fused to HaloTag for protein localization through fluorescence imaging

Depositor

Young J Yoon

Article

Monday et al Neuron. 2022 Aug 17;110(16):2588-2606.e6

Insert

HaloTag fused to mouse Fragile X mental retardation protein (Ctsa Mouse)

Use

Lentiviral

Tags

HaloTag

Promoter

hSyn (human Synapsin promoter)

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_hsyn_NES-his-CaMPARI2-F391W-L398V

Plasmid

#119723

Purpose

AAV vector expressing CaMPARI2_F391W-L398V (Kd = 174nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Thomas Oertner

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Inserts

CaMPARI2
CaMPARI2

Use

AAV

Tags

NES-his

Expression

Mammalian

Mutation

F391W, L398V

Promoter

hSyn1

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+) NRP1 b1b2 (273-586)

Plasmid

#162733

Purpose

Bacterial expression the b1b2 tandem domains from the rat Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

Depositor

Brett Collins

Article

Daly et al Science. 2020 Oct 20. pii: science.abd30

Insert

Human NRP1 b1b2 domains (residues 273-586)

Tags

6His

Expression

Bacterial

Mutation

Codon optimised

Available Since

March 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAAV.CMV.SV40.THBS1-HA-KDEL.SV40(polyA)

Plasmid

#156164

Purpose

Expresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus and KDEL (ER retention) sequence

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

THBS1 with addition of ER-retention sequence KDEL

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA 3.1 mitoAURKA

Plasmid

#157750

Purpose

Expression of AURKA targeted to mitochondria

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Expression

Mammalian

Mutation

Aurora A without the first 30AA + MTS of CoxVIII

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-EGFP-CAAX

Plasmid

#208684

Purpose

Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in neurons

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

membrane-localized enhanced green fluorescent protein EGFP-CAAX

Use

AAV

Promoter

hSyn

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-Tet-On-ARNTL-shRNA3

Plasmid

#166915

Purpose

Lentivirus for inducible knockdown of human ARNTL(BMAL1)

Depositor

Roland Friedel

Article

Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

Insert

ARNTL shRNA (BMAL1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TBK1 gRNA (BRDN0001148392)

Plasmid

#76362

Purpose

3rd generation lentiviral gRNA plasmid targeting human TBK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TBK1 (TBK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
AP1muA single guide RNA

Plasmid

#230030

Purpose

guide RNA for AP1muA tagging at the C-terminus in pX459

Depositor

Francesca Bottanelli

Article

Stockhammer et al J Cell Sci. 2024 Aug 15;137(16):jcs26195

Insert

AP1M1 guide (AP1M1 Human)

Use

CRISPR

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-PPO-Venus

Plasmid

#139502

Purpose

Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

parapinopsin

Tags

Venus tag following 3x Ala linker

Expression

Mammalian

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_hsyn_NES-his-CaMPARI2-H396K-WPRE-SV40

Plasmid

#101062

Purpose

AAV vector expressing CaMPARI2_H396K (Kd = 360nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Insert

CaMPARI2-H396K

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TBK1 gRNA (BRDN0001145663)

Plasmid

#76361

Purpose

3rd generation lentiviral gRNA plasmid targeting human TBK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TBK1 (TBK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-nEF-NpHR3.3-EYFP

Plasmid

#137151

Purpose

Viral expression of NpHR3.3-EYFP

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

NpHR3.3-EYFP

Use

AAV

Mutation

W179F

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1 KanR foundation lox casette

Plasmid

#132388

Purpose

Foundation cassette containing lox sites for cre-mediated exchange of inducible vectors, confers mammalian G418 resistance, targets AAVS1 locus

Depositor

Gerold Schmitt-Ulms

Article

Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

Insert

G418 resistance

Use

CRISPR and Cre/Lox

Expression

Mammalian

Promoter

promoterless

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTBL680 CHYRON3 integration construct

Plasmid

#126448

Purpose

To integrate the CHYRON3 locus at AAVS1 in human cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

pU6/3xLacO-CHYRON3 hgRNA
pCMV-puro

Expression

Mammalian

Mutation

The SpCas9 sgRNA constant region is mutated to ma…

Promoter

CMV and human U6/3xLacO

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CMV.SV40.THBS1-deltaTSR1-HA.SV40(polyA)

Plasmid

#156162

Purpose

Expresses murine Thrombospondin-1 (THBS1) with deletion (AA 383-548) and HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

THBS1 with deletion of amino acid residues 383-548

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge