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We narrowed to 2,311 results for: Kap

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Showing: 2001 - 2050 of 2311 results

  12. pET21b(+)_SARS-CoV-2_3CLpro-Q306A

    Plasmid
    #177334
    Purpose
    bacterial expression of active SARS-CoV-2 3C-like proteinase
    Depositor
    Christopher Overall
    Article
    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Factor Xa site-3xFlag-Myc-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    Nov. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    177334-plasmid-map-sequence-id-348602

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  16. pET21b(+)_SARS-CoV-2_3CLpro_Q306A-HIS

    Plasmid
    #224697
    Purpose
    Bacterial Expression and Purification of SARS-CoV-2 3CLpro active protease with His-tag
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Gly-Gly-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    July 2, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224697-plasmid-map-sequence-id-444276

    Enlarge

  17. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid
    #213026
    Purpose
    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213026-plasmid-map-sequence-id-422069

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  18. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid
    #213030
    Purpose
    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213030-plasmid-map-sequence-id-422062

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  19. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

    Plasmid
    #213031
    Purpose
    Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration
    Depositor
    Donna Romero, Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
    Use
    Luciferase
    Tags
    Firefly luciferase antisense intron (3' UTR)
    Expression
    Mammalian
    Promoter
    pTRE bidirectional tet-on
    Available Since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213031-plasmid-map-sequence-id-422025

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  21. pET21b(+)_SARS-CoV-2_3CLpro-C145A-Q306A

    Plasmid
    #177335
    Purpose
    bacterial expression of inactive SARS-CoV-2 3C-like-C145A proteinase
    Depositor
    Christopher Overall
    Article
    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:
    Insert
    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Factor Xa site-3xFlag-Myc-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    Nov. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    177335-plasmid-map-sequence-id-348601

    Enlarge

  22. pET21b(+)_SARS-CoV-2_3CLpro_C145A-HIS

    Plasmid
    #224698
    Purpose
    Bacterial Expression and Purification of SARS-CoV-2 3CLpro inactive protease with His-tag
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Gly-Gly-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    July 2, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224698-plasmid-map-sequence-id-444264

    Enlarge

  23. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213029
    Purpose
    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213029-plasmid-map-sequence-id-422050

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  24. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213028
    Purpose
    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    E43S D145N double mutant EN- endonuclease catalyt…
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213028-plasmid-map-sequence-id-422046

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  25. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213027
    Purpose
    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    D702Y (RT- reverse transcriptase catalytic dead)
    Promoter
    CMV
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    213027-plasmid-map-sequence-id-452358

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  29. PA-GFP-α-Tubulin

    Plasmid
    #213900
    Purpose
    Imaging of Tubulin using Photoactivatable GFP
    Depositor
    Lukas Kapitein
    Insert
    PA-GFP-α-Tubulin
    Tags
    Photoactivatable GFP
    Expression
    Mammalian
    Available Since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213900-plasmid-map-sequence-id-426213

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  31. pET11a-p65(19-325)

    Plasmid
    #44744
    Depositor
    Gourisankar Ghosh
    Insert
    p65 (Rela Mouse)
    Expression
    Bacterial
    Mutation
    AA 19-325
    Available Since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only

  32. pET12a-JOH1

    Plasmid
    #47098
    Depositor
    Marina Ramirez-Alvarado
    Insert
    JOH1 (IGKV3-20 Human)
    Tags
    ompT
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  33. pET12a-DAH

    Plasmid
    #47096
    Depositor
    Marina Ramirez-Alvarado
    Insert
    DAH (IGKV3-20 Human)
    Tags
    ompT
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  36. AbVec_IGKC

    Plasmid
    #183702
    Purpose
    Encode human Ig kappa chain constant region. For cloning variable region and mAb expression.
    Depositor
    David Nemazee
    Insert
    IGKC (IGKC )
    Expression
    Mammalian
    Promoter
    SP6
    Available Since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    183702-plasmid-map-sequence-id-358531

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  37. pRS313_MSN5-LY00045

    Plasmid
    #232904
    Purpose
    Plasmid carrying MSN5 from LY00045 (S288C) with its native promoter and terminator.
    Depositor
    Jolanda Van Leeuwen
    Insert
    MSN5 (MSN5 Budding Yeast)
    Expression
    Yeast
    Available Since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232904-plasmid-map-sequence-id-462086

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  38. pJK637

    Plasmid
    #72437
    Purpose
    Acetobacter aceti 1023 mutS, partial gene
    Depositor
    T. J. Kappock
    Insert
    DNA mismatch repair protein MutS (partial gene)
    Expression
    Bacterial
    Mutation
    deletes nt 1-767 and nt 2086-2655 of mutS gene
    Promoter
    n/a
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72437-plasmid-map-sequence-id-132658

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  40. AAV-CAG-K-SPOTIT

    Plasmid
    #191488
    Purpose
    Green fluorescent-based opioid sensor for the kappa opioid receptor in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    K-SPOTIT
    Use
    AAV
    Promoter
    CAG
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191488-plasmid-map-sequence-id-377866

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  41. R777-E293 Hs.RPS6KA3

    Plasmid
    #70577
    Purpose
    Gateway ORF clone of human RPS6KA3 [NM_004586.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RPS6KA3 (RPS6KA3 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70577-plasmid-map-sequence-id-181747

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  42. R777-E291 Hs.RPS6KA2

    Plasmid
    #70575
    Purpose
    Gateway ORF clone of human RPS6KA2 [NM_021135.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RPS6KA2 (RPS6KA2 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70575-plasmid-map-sequence-id-126024

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  43. R777-E294 Hs.RPS6KA3-nostop

    Plasmid
    #70578
    Purpose
    Gateway ORF clone of human RPS6KA3 [NM_004586.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RPS6KA3 (RPS6KA3 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70578-plasmid-map-sequence-id-126027

    Enlarge

  44. R777-E292 Hs.RPS6KA2-nostop

    Plasmid
    #70576
    Purpose
    Gateway ORF clone of human RPS6KA2 [NM_021135.4] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RPS6KA2 (RPS6KA2 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70576-plasmid-map-sequence-id-181745

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  45. pRS313_MSN5-LY00018

    Plasmid
    #232903
    Purpose
    Plasmid carrying MSN5 from LY00018 (YJM975) with its native promoter and terminator.
    Depositor
    Jolanda Van Leeuwen
    Insert
    MSN5 (MSN5 Budding Yeast)
    Expression
    Yeast
    Mutation
    G1563A
    Available Since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232903-plasmid-map-sequence-id-462290

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  46. PTPRK

    Plasmid
    #38968
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPRK (PTPRK Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  47. AAV-CAG-K-red-SPOTIT

    Plasmid
    #191490
    Purpose
    Red fluorescent-based opioid sensor for the kappa opioid receptor in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    K-red-SPOTIT
    Use
    AAV
    Promoter
    CAG
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191490-plasmid-map-sequence-id-377865

    Enlarge

  48. pEBG-II-I Y248,249,611F

    Plasmid
    #22251
    Depositor
    Ananda Roy
    Insert
    TFII-I (GTF2I Human)
    Tags
    GST
    Expression
    Mammalian
    Mutation
    Tyrosine 248, 249 and 611 mutated to Phenylalanine
    Available Since
    Nov. 13, 2009
    Availability
    Academic Institutions and Nonprofits only

  49. AAV-CAG-K-SPOTIT(R271F)

    Plasmid
    #191492
    Purpose
    Green fluorescent-based opioid sensor for the kappa opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    K-SPOTIT(R271F)
    Use
    AAV
    Mutation
    R271F in OR
    Promoter
    CAG
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191492-plasmid-map-sequence-id-377952

    Enlarge

  50. hsKIF5B(1-560)-HaloTag-FRB

    Plasmid
    #213899
    Purpose
    Imaging of KIF5B fused to HaloTag
    Depositor
    Lukas Kapitein
    Insert
    hsKIF5B(aa1-560)-HaloTag-FRB (KIF5B Human)
    Tags
    HaloTag
    Expression
    Mammalian
    Available Since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213899-plasmid-map-sequence-id-426209

    Enlarge

Showing: 2001 - 2050 of 2311 results