We narrowed to 1,147 results for: ENDO 2
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Plasmid#105523PurposeT7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmCherryMutationdeletion of RING domain (aa124-462)PromoterT7Available SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC84-pCR8-dCas9
Plasmid#48218PurposedCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A)
UseCRISPR; Gateway donorTagsHA TagMutationD10A;H840AAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
Psicheck2 COLIA1 full length 3'UTR
Plasmid#26993DepositorInsertCOL1A1 Full length 3' UTR (COL1A1 Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMammalianAvailable SinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mEGFP-mTrim21ΔRING-Box
Plasmid#105520PurposeT7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted RING-box mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmEGFPMutationdeletion of RING domain (aa124-462)PromoterT7Available SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC5-dual-dCas9VP48-sgTetO
Plasmid#48237PurposeDual expression construct expressing both dCas9VP48 and sgTetO from separate promotersDepositorInsertdCas9VP48 and sgTetO
UseCRISPRTagsHA-tag and VP48ExpressionMammalianMutationD10A H840A (catalytically inactive)Available SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAC92-pmax-dCas9VP96
Plasmid#48224PurposedCas9VP96 on pmax expression vectorDepositorInsertdCas9(D10A;H840A) fusion with VP96 activation domain
UseCRISPRTagsHA Tag and VP96ExpressionMammalianMutationD10A;H840APromoterCAGGSAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-ATL2-R244Q
Plasmid#109023Purposestable lentiviral expression of cDNADepositorInsertATL2-R244Q (ATL2 Human)
UseLentiviralTagseGFPExpressionMammalianMutationArg244Gln mutation results in dimerization defectPromoterEIF-1a promoterAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mEGFP-mTrim21ΔPRYSPRY
Plasmid#105521PurposeT7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted PRYSPRY domain mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmEGFPMutationdeletion of PRYSPRY domain (aa1-281)PromoterT7Available SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Psicheck2 MERTK full length 3'UTR
Plasmid#26992DepositorInsertMERTK Full length 3' UTR (MERTK Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMammalianAvailable SinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
Psicheck2 Sox4 mut 3'UTR oligo
Plasmid#26988DepositorInsertSox4 mut UTR oligo (SOX4 Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMammalianMutationbp 449-509 of SOX4 3'UTR; mut @ bp 483Available SinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRa_all-in-one_Bip gRNA1
Plasmid#183696PurposeExpresses gRNA targeting chinise hamster Bip (Hspa5) with CRISPRa components and mCherry, blasticidin selectiveDepositorInsertgRNA targeting chinese hamster Bip (Hspa5)
UseCRISPRAvailable SinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-dora[B]_rescue_construct
Plasmid#190638PurposePuromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
Tags3xHAExpressionInsectMutationChanged threonine 295 to methioninePromoterActin-5cAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-dora[A]_rescue_construct
Plasmid#190640PurposePuromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
Tags3xHAExpressionInsectMutationTruncated to contain amino acids 1-945PromoterActin-5cAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-ATL2-deltaTM
Plasmid#109021Purposestable lentiviral expression of cDNADepositorInsertATL2-deltaTM (ATL2 Human)
UseLentiviralTagseGFPExpressionMammalianMutationtruncated form without C-terminal ER transmembran…PromoterEIF-1a promoterAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dll4CE2-P1Egfp
Plasmid#90147Purposecontains dll4 enhancer 2 sequence, and dll4 specific gene promoter driving expression of eGFPDepositorAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgATL2-1
Plasmid#109008PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-161K enhancer-NR2F2-MUT-minP:fLuc-TK:rLuc
Plasmid#138368PurposeLentiviral plasmid contains 161k modified human enhancer with with NR2F2 binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoterDepositorInsert161K human enhancer, with NR2F2 binding sites mutated, driving firefly enhancer and TK driving renilla luciferase
UseLentiviralExpressionMammalianAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dll4CE2-basEgfp
Plasmid#90144Purposecontains dll4 enhancer 2 sequence and a basal promoter driving expression of eGFPDepositorAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBrain-ch-TOGKDP(L1939A,L1942A)-GFP-shch-TOG
Plasmid#69114PurposeDual-promoter plasmid to express knockdown-proof human ch-TOG with L1939A and L1942A substitutions tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.DepositorInsertsUseRNAiTagsEGFPExpressionMammalianMutationL1939A and L1942A amino acid substitutions. Sile…PromoterCMVAvailable SinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
TOPO mMecp2-NLucTom
Plasmid#159282PurposeTo tag endogenously and c-terminally mouse Mecp2 with two reporters: NanoLuciferase and TdTomato. It contains 2 homology arms from a castaneus background surrounding the STOP codon.DepositorInsertMecp2 (Mecp2 Mouse)
TagsNluciferase-P2A-TdTomatoExpressionMammalianMutationNluciferase-TdTomato fusionAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only