We narrowed to 1,068 results for: FIB

1. pCMV_SEPT9_i1 R10A R15A-msfGFP

   Plasmid

   #180336

   Purpose

   mammalian expression of human SEPT9_i1 R10A R15A fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN9_v1 (SEPTIN9 Human)
   

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i1 R10A R15A

   Promoter

   CMV

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV_SEPT9_i1 S12A S13A-msfGFP

   Plasmid

   #180337

   Purpose

   mammalian expression of human SEPT9_i1 S12A S13A fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN9_v1 (SEPTIN9 Human)
   

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i1 S12A S13A

   Promoter

   CMV

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP Kind2 S159/181/666E

   Plasmid

   #105314

   Purpose

   To study altered downstream functions of phospholylation-site mimetic kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S159/181/666E

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4-AARE-luc2P-Hygro

   Plasmid

   #101787

   Purpose

   Luferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)

   Depositor

   Seiichi Oyadomari

   Article

   Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

   Insert

   3xAARE
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Promoter

   minimal TATA-box promoter with low basal activity

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTight-9-124-BclxL

   Plasmid

   #60857

   Purpose

   2nd generation lentiviral transfer plasmid. Expresses a synthetic cluster of miR-9/9* and miR-124 fused to Bcl-xL under a doxycycline-inducible promoter

   Depositor

   Andrew Yoo

   Article

   Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

   Insert

   miR-9/9*-miR-124-BCLxL (BCL2L1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   pTight

   Available Since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-28a(+)-FGF2-G3

   Plasmid

   #135521

   Purpose

   Engineered form of fibroblast growth factor 2 with improved thermostability

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   fibroblast growth factor 2 (FGF2 Human)
   

   Tags

   6x His tag and Agg Thrombin Tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 Promoter

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

   Plasmid

   #99278

   Purpose

   Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance

   Depositor

   Jordan Miller

   Article

   Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

   Inserts

   H2B-mVenus-IRES-mCherry
   Puro
   

   Use

   Lentiviral

   Tags

   H2B, IRES, PGK, mCherry, and mVenus

   Promoter

   EF1 alpha-human and PGK-mouse

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSAM2_mCherry_Gata6

   Plasmid

   #72694

   Purpose

   Doxycyline regulated expression of Gata6 in mammalian cells

   Depositor

   Timothy Kamp

   Article

   Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

   Insert

   Gata 6 (Gata6 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV-Doxycycline

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3. Flag-CSL WT

   Plasmid

   #167004

   Purpose

   Mammalian expression of CSL WT

   Depositor

   Gian-Paolo Dotto

   Article

   Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

   Insert

   CSL (RBPJ Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-CMV-Blast-DNTGFBR2-HA

    Plasmid

    #130888

    Purpose

    Expresses dominant negative mutant human TGFBR2

    Depositor

    Gian-Paolo Dotto

    Article

    Bordignon et al Cell Rep. 2019 Aug 27;28(9):2358-2372.e6

    Insert

    TGFBR2 (TGFBR2 Human)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Dominant Negative receptor lacking cytosolic cata…

    Promoter

    CMV

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCX-HO1-2A-EGFP

    Plasmid

    #74672

    Purpose

    Mammalian expression of HO1 and EGFP

    Depositor

    Roberto Giovannoni

    Article

    Cinti et al PLoS One. 2015 Oct 29;10(10):e0141933. d

    Insert

    HO1 (HMOX1 Human)
    

    Tags

    2A-EGFP

    Expression

    Mammalian

    Promoter

    pCAGGS

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-IDO1-p2a-eGFP

    Plasmid

    #187026

    Purpose

    Expresses IDO1 and GFP (seperately) in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Maassen et al Free Radic Biol Med. 2022 Aug 1;188:287-

    Insert

    Indoleamine 2,3-dioxygenase 1 (IDO1 Human)
    

    Tags

    P2A-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. ATX3-13Q

    Plasmid

    #184248

    Purpose

    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)

    Depositor

    Sandra Macedo Ribeiro

    Article

    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

    Insert

    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Human, Synthetic)
    

    Tags

    6x His-Tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    C-terminal codon optimization for protein expres…

    Promoter

    T7 promoter

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-RNF2-ngRNA+41_EF1a-puroR

    Plasmid

    #207358

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    RNF2 +41 ngRNA
    

    Use

    Lentiviral

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21-10XHis-GST-HRV-dL5

    Plasmid

    #73214

    Purpose

    Bacterial expression vector for dL5(E52D) protein with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44

    Insert

    EGFR (EGFR Human, Schistosoma japonicum)
    

    Tags

    10XHis-GST-HRV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSH1/M-FGFR1-Fv-Fvls-E

    Plasmid

    #15285

    Depositor

    David Spencer

    Article

    Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

    Insert

    FGFR1 kinase, FKBP12v36 (Fgfr1 Mouse)
    

    Tags

    HA epitope and Myristoylation-targeting domain c-…

    Expression

    Mammalian

    Mutation

    FGFR1 cytoplasmic domain FKBP12 F36V

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3. Flag-CSL R192H

    Plasmid

    #167005

    Purpose

    Mammalian expression of CSL R192H

    Depositor

    Gian-Paolo Dotto

    Article

    Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

    Insert

    CSL (RBPJ Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    R192H

    Promoter

    CMV

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

    Plasmid

    #99277

    Purpose

    Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance

    Depositor

    Jordan Miller

    Article

    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

    Inserts

    H2B-mOrange2-IRES-EGFP
    Puro
    

    Use

    Lentiviral

    Tags

    EGFP, H2B, IRES, PGK, and mOrange2

    Promoter

    EF1 alpha-human and PGK-mouse

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB-tetpA(hBCL2-miR9/9*-124)-iRPT

    Plasmid

    #154881

    Purpose

    Doxycycline-dependently expresses human BCL2 and murine miR9/9*-124. Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.

    Depositor

    Hideyuki Okano

    Article

    Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

    Inserts

    iRFP713
    Puromycin resistance gene
    rtTAM2
    BCL2 (BCL2 Human)
    miR9/9*-124
    

    Expression

    Mammalian

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPBCAG-rtTAM2-2A-SOX17-GR-IH

    Plasmid

    #165079

    Purpose

    Ubiquitously expresses rtTAM2, dexamethasone-inducible human SOX17, and hygromycin-resistance gene.

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Reports. 2021 Mar 23. pii: S22

    Inserts

    rtTA-M2
    SOX17 (SOX17 Human)
    

    Use

    Piggybac transposition

    Tags

    Glucocorticoid Receptor

    Expression

    Mammalian

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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