We narrowed to 165,191 results for: addgene

1. 10X PRE TK luc

   Plasmid

   #206162

   Purpose

   Luciferase reporter construct containing 10 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

   Depositor

   Renee van Amerongen

   Article

   Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

   Insert

   10X PRE TK luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   min TK

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. 8X PRE TK luc

   Plasmid

   #206161

   Purpose

   Luciferase reporter construct containing 8 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

   Depositor

   Renee van Amerongen

   Article

   Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

   Insert

   8X PRE TK luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   min TK

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 6X PRE TK luc

   Plasmid

   #206160

   Purpose

   Luciferase reporter construct containing 6 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

   Depositor

   Renee van Amerongen

   Article

   Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

   Insert

   6X PRE TK luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   min TK

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX330_EZH2gRNA15

   Plasmid

   #199587

   Purpose

   Contains guide RNA to 5' end of mouse EZH2 gene

   Depositor

   Mauro Calabrese

   Article

   Cherney et al bioRxiv. 2023 Apr 3:2023.04.03.535391. d

   Insert

   EZH2 (Ezh2 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Expression

   Bacterial and Mammalian

   Available Since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330_EZH2gRNA16

   Plasmid

   #199588

   Purpose

   Contains guide RNA to 3' end of mouse EZH2 gene

   Depositor

   Mauro Calabrese

   Article

   Cherney et al bioRxiv. 2023 Apr 3:2023.04.03.535391. d

   Insert

   EZH2 (Ezh2 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Expression

   Bacterial and Mammalian

   Available Since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330_EZH2gRNA17

   Plasmid

   #199589

   Purpose

   Contains guide RNA to 3' end of mouse EZH2 gene

   Depositor

   Mauro Calabrese

   Article

   Cherney et al bioRxiv. 2023 Apr 3:2023.04.03.535391. d

   Insert

   EZH2 (Ezh2 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Expression

   Bacterial and Mammalian

   Available Since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. attB53_SNCB-_attB53

   Plasmid

   #183615

   Purpose

   RMCE vector to shuttle puromycin resistance (+), anti-GFP SynNotch receptor (+), tagBFP (-) and TRE-mCherry (-) cassettes into attP50-flanked landing pad (Addgene 183609). (+) = sense (-) = antisense.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Inserts

   LaG17 GFP nanobody SynNotch tTA
   TRE-mCherry-SV40pA
   tagBFP
   

   Use

   Rmce

   Tags

   Myc and human CD8a signal sequence

   Expression

   Mammalian

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFUGW-shRIIα

   Plasmid

   #183454

   Purpose

   sgRNA targeting rat PKA-RIIα subunits

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   sgRNA targeting rat PKA-RIIα (Prkar2a Rat)
   

   Use

   Lentiviral

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFUGW-scrambled

   Plasmid

   #183455

   Purpose

   This control vector contains a scrambled version of the targeting sequence used in the pFUGW-shRIIα construct

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   scrambled sgRNA
   

   Use

   Lentiviral

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330_gRNA_IG-DMR-1

    Plasmid

    #159931

    Purpose

    Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse IG-DMR
    

    Use

    CRISPR and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6, CBh

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330_gRNA_IG-DMR-2

    Plasmid

    #159932

    Purpose

    Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse IG-DMR
    

    Use

    CRISPR and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6, CBh

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSLIK_hygro_p53(R280K)_V5

    Plasmid

    #136540

    Purpose

    Lentiviral expression vector for an inducible p53(R280K)-V5

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    p53(R280K) (TP53 Human)
    

    Use

    Lentiviral; Destinatioin vector for gateway cloni…

    Tags

    V5

    Expression

    Mammalian

    Mutation

    p53(R280K)V5 was cloned from pLenti6-p53-R280K-V5…

    Available Since

    June 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEN_TTmiRc2_p53(R280K)_V5

    Plasmid

    #136525

    Purpose

    Used as a donor vector to clone into pSLIK

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    p53(R280K) (TP53 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    V5

    Mutation

    p53(R280K)V5 was cloned from pLenti6-p53-R280K-V5…

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA

    Plasmid

    #154868

    Purpose

    Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry

    Depositor

    Ulrik Gether

    Has Service

    AAV Retrograde and AAV8

    Insert

    hM3D(Gq)-mCherry
    

    Use

    AAV; Flp-frt

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    Human Synapsin-1

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUltra-Plus-H2B-mTagBFP2

    Plasmid

    #161790

    Purpose

    3rd generation lentiviral bicistronic vector for mammalian expression of H2B-mTagBFP2 and one gene of interest

    Depositor

    Jorge Luis Arias-Arias

    Insert

    H2B-mTagBFP2
    

    Use

    Lentiviral; Bicistronic

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    I174A in the mTagBFP2 protein according to PLoS O…

    Promoter

    UbC and UbC promoter

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZC06 (pFA6a-PSc-6xGly-PrtA-KanMX)

    Plasmid

    #44090

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    6xGly linker, ADH1 transcription terminator, KanM…

    Available Since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV CMV FST-FLAG

    Plasmid

    #175167

    Purpose

    Lentiviral expression of human FST-FLAG

    Depositor

    Larry Gerace

    Insert

    FST (FST Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. 7TFC

    Plasmid

    #24307

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    7xTcf-FFluc // SV40-mCherry
    

    Use

    Lentiviral

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWT015a

    Plasmid

    #96878

    Purpose

    GFP reporter

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    GFP
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-Ef1a-fDIO-JRGECO1a

    Plasmid

    #128317

    Purpose

    Expresses JRGECO1a under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

    Depositor

    Patricia Jensen

    Has Service

    AAV1

    Insert

    NES-JRGECO1a
    

    Use

    AAV

    Tags

    His Tag

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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