We narrowed to 1,270 results for: grna cloning vector

1. pCFD3-MS2tail-Scaffold

   Plasmid

   #78906

   Purpose

   Vector for expressing a single sgRNA with Scaffold MS2 tail, under U6:3 promoter

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Sept. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMGC

   Plasmid

   #112812

   Purpose

   PCR template vector for amplifying gRNA(F+E)-OstRNA fragment

   Depositor

   Chun Han

   Article

   Poe et al Genetics. 2018 Nov 30. pii: genetics.118

   Insert

   gRNAcore(F+E)-tRNA
   

   Expression

   Bacterial

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTBL3346 PB-nicking guide-blast

   Plasmid

   #226665

   Purpose

   PiggyBac cargo vector encoding protective nicking sgRNA marked with blasticidin

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Insert

   protective nicking sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDe_Cas9_KANA_R160a

   Plasmid

   #173756

   Purpose

   Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160a

   Depositor

   Kristina Gruden

   Article

   Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

   Insert

   Cas9_sgRNA1/R160a_sgRNA2/R160a
   

   Use

   CRISPR

   Promoter

   AtU6 &StU6

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDe_Cas9_KANA_R160b

   Plasmid

   #173757

   Purpose

   Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160b

   Depositor

   Kristina Gruden

   Article

   Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

   Insert

   Cas9_sgRNA1/R160b_sgRNA2/160b
   

   Use

   CRISPR

   Promoter

   AtU6 &StU6

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDe_Cas9_KANA_R390a

   Plasmid

   #173758

   Purpose

   Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA390a

   Depositor

   Kristina Gruden

   Article

   Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

   Insert

   Cas9_sgRNA1/390a_sgRNA2/390a
   

   Use

   CRISPR

   Promoter

   AtU6 &StU6

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. MS2-adRNA (5'GAC)

   Plasmid

   #170127

   Purpose

   Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   MS2-ADAR2(5'GAC)-MS2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Human U6 and mouse U6

   Availability

   Academic Institutions and Nonprofits only

   

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8. MS2-adRNA (3'GAC)

   Plasmid

   #170129

   Purpose

   Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   MS2-ADAR2(3'GAC)-MS2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Human U6 and mouse U6

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB_rtTA_BsmBI

   Plasmid

   #126028

   Purpose

   PiggyBac cargo vector expressing rtTA, contains BsmBI sites for sgRNA cloning

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Type

   Empty backbone

   Use

   CRISPR; Piggybac

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6-crRNA(SOD1)

    Plasmid

    #109321

    Purpose

    AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human SOD1

    Depositor

    Feng Zhang

    Article

    Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

    Insert

    mCherry-KASH (SOD1 Human)
    

    Use

    AAV

    Promoter

    hSyn1

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSIREN-RetroQ-BPTF-sh28

    Plasmid

    #73669

    Purpose

    pSIREN-RetroQ vector containing shRNA sequence to BPTF

    Depositor

    Joe Landry

    Insert

    shRNA 28 to BPTF (BPTF, Bptf Human, Mouse)
    

    Use

    Retroviral

    Available Since

    March 22, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pSIREN-RetroQ-BPTF-sh27

    Plasmid

    #73668

    Purpose

    pSIREN-RetroQ vector containing shRNA sequence to BPTF

    Depositor

    Joe Landry

    Insert

    shRNA #27 to BPTF (BPTF, Bptf Human, Mouse)
    

    Use

    Retroviral

    Available Since

    April 13, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pLSB-NAT

    Plasmid

    #166698

    Purpose

    Combined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.

    Depositor

    Robin Allshire

    Article

    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

    Inserts

    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    

    Expression

    Yeast

    Promoter

    adh15 promoter and tRNA promoter

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLSB-KAN

    Plasmid

    #166700

    Purpose

    Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.

    Depositor

    Robin Allshire

    Article

    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

    Inserts

    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    

    Expression

    Yeast

    Promoter

    adh15 promoter and tRNA Ser promoter

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLSB-HYG

    Plasmid

    #166699

    Purpose

    Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.

    Depositor

    Robin Allshire

    Article

    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

    Inserts

    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    

    Expression

    Yeast

    Promoter

    adh15 promoter and tRNA promoter

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX458-tRNA-SpCas9

    Plasmid

    #195183

    Purpose

    Vector for transient Cas9 and EGFP expression. Small tRNA promoter for sgRNA cloning by GoldenGate.

    Depositor

    Stephanie Roessler

    Insert

    Cas9
    

    Use

    CRISPR

    Promoter

    tRNA-Gln

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEJS1964 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-miniU6-Rosa26

    Plasmid

    #199262

    Purpose

    Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one miniU6 driven sgRNA targeting mouse Rosa26 gene

    Depositor

    Erik Sontheimer

    Article

    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

    Insert

    NmeABE8e
    

    Use

    AAV and CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    U1a

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEJS-HZ54 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26

    Plasmid

    #199265

    Purpose

    Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene

    Depositor

    Erik Sontheimer

    Article

    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

    Insert

    NmeABE8e
    

    Use

    AAV and CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    U1a

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEJS1830 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-U6-Fah

    Plasmid

    #199263

    Purpose

    Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting the point mutation in the mouse Fah gene of a HT1 mouse model

    Depositor

    Erik Sontheimer

    Article

    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

    Insert

    NmeABE8e
    

    Use

    AAV and CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    U1a

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. epiCRISPRn

    Plasmid

    #135961

    Purpose

    Expresses SpCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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