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Showing: 801 - 820 of 9605 results

  2. pCSmChnERT(#99)

    Plasmid
    #184063
    Purpose
    cyclofen-inducible mCherry nuclear relocation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Sinha et al Chembiochem. 2010 Mar 22;11(5):653-63. d
    Insert
    mCherry-nls-ERT2
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    184063-plasmid-map-sequence-id-370311

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  5. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

    Plasmid
    #119816
    Purpose
    3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells
    Depositor
    Ethan Abel
    Article
    Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:
    Inserts
    Luciferase
    Puromycin
    Use
    Lentiviral and Luciferase
    Tags
    EGFP and V5
    Expression
    Mammalian
    Mutation
    Stop codon removed in the frame of the V5 frame v…
    Promoter
    CMV/TO and Human Phosphoglycerate kinase
    Available since
    Jan. 2, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119816-plasmid-map-sequence-id-372950

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  11. AAV-hSyn-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

    Plasmid
    #177345
    Purpose
    AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from Synapsin promoter
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    dead hSaCas9
    Use
    AAV and CRISPR
    Tags
    3x GCN4 peptide, 3xHA, and NLS
    Expression
    Mammalian
    Mutation
    D10A, N580A
    Promoter
    Synapsin
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177345-plasmid-map-sequence-id-353619

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  13. INS-GFP-CD19-EPT

    Plasmid
    #210469
    Purpose
    Donor plasmid for knock-in GFP-CD19 into the human INS locus
    Depositor
    Kai Wang
    Article
    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do
    Insert
    INS homologous recombination arms with a GFP-CD19 and EF1alpha-drived puromycin-NLS-tdTomato selection cassette (INS Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    210469-plasmid-map-sequence-id-419052

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  14. pTV001

    Plasmid
    #130265
    Purpose
    EatA expression plasmid containing 8His encoding region in permissive site of eatA gene
    Depositor
    James Fleckenstein
    Article
    Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do
    Insert
    eatA::His8
    Use
    Tags
    polyhistidine (internal)
    Expression
    Bacterial
    Mutation
    8His polyhistidine encoding region and XhoI site …
    Promoter
    araBAD
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    130265-plasmid-map-sequence-id-257613

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  15. AAV-hSyn-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

    Plasmid
    #177360
    Purpose
    AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression from Synapsin promoter
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Inserts
    tdTomato
    Chimeric guide for SaCas9
    Use
    AAV
    Tags
    P2A and a tetracycline-controlled transactivator …
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsine promoter and U6 promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177360-plasmid-map-sequence-id-353669

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  16. AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

    Plasmid
    #177365
    Purpose
    AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Inserts
    tdTomato
    Chimeric guide for SaCas9
    Use
    AAV
    Tags
    P2A and a tetracycline-controlled transactivator …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6 promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177365-plasmid-map-sequence-id-353664

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  17. AAV-CMV-scFVtet2_bGHpA

    Plasmid
    #177352
    Purpose
    AAV expression of scFV-fused catalytic domain of TET2 for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    Tet Methylcytosine Dioxygenase 2 (TET2 Human)
    Use
    AAV
    Tags
    myc and scFV
    Expression
    Mammalian
    Mutation
    Catalytic domains of human TET2 (1129–1936 and 14…
    Promoter
    CMV promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177352-plasmid-map-sequence-id-353673

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  19. B2M-SDMutation-EhT

    Plasmid
    #215546
    Purpose
    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out
    Depositor
    Kai Wang
    Article
    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do
    Insert
    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived hygromycin-NLS-tdTomato selection cassette (B2M Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    The second base of the first intron of B2M (From …
    Promoter
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    215546-plasmid-map-sequence-id-425336

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  20. B2M-SDMutation-EPG

    Plasmid
    #215545
    Purpose
    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out
    Depositor
    Kai Wang
    Article
    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do
    Insert
    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived puromycin-GFP selection cassette (B2M Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    The second base of the first intron of B2M (From …
    Promoter
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    215545-plasmid-map-sequence-id-425338

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Showing: 801 - 820 of 9605 results