Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 15,909 results for: genome

Showing: 8181 - 8200 of 15909 results

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pYPQ291-D573A-SRDX

Plasmid

#129683

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

BthCas12b-SRDX

Use

CRISPR; Gateway compatible bthcas12b entry clone

Expression

Plant

Mutation

D573A; BthCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aa3.8.3

Plasmid

#136374

Purpose

Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is used

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aac.3

Plasmid

#136377

Purpose

Gateway entry clone for AacCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold with MS2 at the third stem loop is used

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aa1.2.3

Plasmid

#136372

Purpose

Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 1.2 with MS2 at the third stem loop is used

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAWp63-clone3-12 (OptiHF-SpCas9)

Plasmid

#131737

Purpose

Expresses OptiHF-SpCas9 in mammalian cells

Depositor

Alan Siu Lun Wong

Article

Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

Insert

OptiHF-SpCas9

Use

Lentiviral

Tags

Flag

Mutation

Q695A+K848A+E923M+T924V+Q926A

Promoter

EFS

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Rictor-g1)-PGKpuroBFP-W

Plasmid

#105041

Purpose

Lentiviral gRNA plasmid targeting mouse Rictor , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Rictor (Rictor Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Rictor-g2)-PGKpuroBFP-W

Plasmid

#105042

Purpose

Lentiviral gRNA plasmid targeting mouse Rictor , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Rictor (Rictor Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Zfp219-g1)-PGKpuroBFP-W

Plasmid

#105021

Purpose

Lentiviral gRNA plasmid targeting mouse Zfp219 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Zfp219 (Zfp219 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Ndufa2-g1)-PGKpuroBFP-W

Plasmid

#105030

Purpose

Lentiviral gRNA plasmid targeting mouse Ndufa2 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Ndufa2 (Ndufa2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Ndufa2-g2)-PGKpuroBFP-W

Plasmid

#105031

Purpose

Lentiviral gRNA plasmid targeting mouse Ndufa2 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Ndufa2 (Ndufa2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Vps25-g1)-PGKpuroBFP-W

Plasmid

#105033

Purpose

Lentiviral gRNA plasmid targeting mouse Vps25 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Vps25 (Vps25 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Tcf7l1-g1)-PGKpuroBFP-W

Plasmid

#105015

Purpose

Lentiviral gRNA plasmid targeting mouse Tcf7l1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

gRNA targeting Tcf7l1 (Tcf7l1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Tcf7l1-g2)-PGKpuroBFP-W

Plasmid

#105016

Purpose

Lentiviral gRNA plasmid targeting mouse Tcf7l1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Tcf7l1 (Tcf7l1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Pggt1b-g1)-PGKpuroBFP-W

Plasmid

#105018

Purpose

Lentiviral gRNA plasmid targeting mouse Pggt1b , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Pggt1b (Pggt1b Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Znrf1-g1)-PGKpuroBFP-W

Plasmid

#105020

Purpose

Lentiviral gRNA plasmid targeting mouse Znrf1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Znrf1 (Znrf1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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gh114

Plasmid

#106784

Purpose

expression of gRNA targeting B4GALNT2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B4GALNT2 (B4GALNT2 Human)

Expression

Mammalian

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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gh25

Plasmid

#106706

Purpose

expression of gRNA targeting POMGNT1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

POMGNT1 (POMGNT1 Human)

Expression

Mammalian

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a-3NLS-Sox2TALE-GCN4

Plasmid

#120548

Purpose

Express 3xNLS-Sox2 TALE-GCN4 engineered to bind a site in the human SOX2 gene

Depositor

Yu Wang

Article

Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

Insert

NLS, Sox2 TALE, GCN4 (SOX2 Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1α

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJET1.2_Nkx3-2_Anti-Sense

Plasmid

#124438

Purpose

Plasmid for anti-sense in situ probe in vitro transcription

Depositor

Yingguang Frank Chan

Article

Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

Insert

NK3 homeobox 2 (Nkx3-2 Mouse)

Use

In situ probe

Promoter

T7

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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gh168

Plasmid

#106830

Purpose

expression of gRNA targeting A4GNT

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

A4GNT (A4GNT Human)

Expression

Mammalian

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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Showing: 8181 - 8200 of 15909 results

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