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pHS641_Flex-Cas12a-ABE

Plasmid

#244846

Purpose

Mammalian expression of Flex-Cas12a adenine base editor

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Flex-dCas12a-ABE

Use

CRISPR

Tags

SV40 NLS-ABE8e

Expression

Mammalian

Mutation

G146R/R182V/D535G/S551F/D665N/E795Q/D832A

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SpRY-IP

Plasmid

#235994

Purpose

Expresses Cas9-SpRY in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

SpRY

Use

CRISPR

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pACUH 5-HT2BR-7xGFP11-HA

Plasmid

#221840

Purpose

Plasmid for generating 10xUAS-5-HT2BR-7xGFP11-HA transgenic flies with attP/attB integration. It carries the attB sequence.

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

5-HT2BR (CG42796 Fly)

Tags

7xGFP11-HA

Expression

Insect

Promoter

hsp70

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn:FLEX-Soma-jGCaMP8m

Plasmid

#239670

Purpose

Cre-dependent expression of Soma-jGCaMP8m under hSyn promoter.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Soma-jGCaMP8m

Use

AAV

Tags

6xHis and EE-RR

Expression

Mammalian

Promoter

hSyn

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn:FLEX-Soma-jGCaMP8s

Plasmid

#239669

Purpose

Cre-dependent expression of Soma-jGCaMP8s under hSyn promoter.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Soma-jGCaMP8s

Use

AAV

Tags

6xHis and EE-RR

Expression

Mammalian

Promoter

hSyn

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn:FLEX-Soma-jGCaMP8f

Plasmid

#239671

Purpose

Cre-dependent expression of Soma-jGCaMP8f under hSyn promoter.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Soma-jGCaMP8f

Use

AAV

Tags

6xHis and EE-RR

Expression

Mammalian

Promoter

hSyn

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_V5-nb-KS

Plasmid

#238266

Purpose

For overexpression of V5-nb-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

V5-nb-KS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1_-DIO-Varnam1-WPRE

Plasmid

#240054

Purpose

Cre dependent Red fluorescent voltage indicator for mammalian expression

Depositor

Mark Schnitzer

Article

Haziza et al Cell, 188, 1-23, August 7, 2025. doi: 10

Insert

VARNAM1

Use

AAV

Promoter

Ef1a

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-Varnam2-WPRE

Plasmid

#240055

Purpose

Cre dependent Red fluorescent, negative response-polarity voltage indicator for mammalian expression

Depositor

Mark Schnitzer

Article

Haziza et al Cell, 188, 1-23, August 7, 2025. doi: 10

Insert

VARNAM2

Use

AAV

Promoter

Ef1a

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pPSmF

Plasmid

#226912

Purpose

Minimal plasmid for Plant STARR-seq with enhancer candidates in forward orientation

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2025 Jun 4;37(6):koaf084. do

Type

Empty backbone

Expression

Plant

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pPSmR

Plasmid

#226913

Purpose

Minimal plasmid for Plant STARR-seq with enhancer candidates in reverse orientation

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2025 Jun 4;37(6):koaf084. do

Type

Empty backbone

Expression

Plant

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_V5-nb-noKS

Plasmid

#238265

Purpose

For overexpression of V5-nb-noKS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

V5-nb-noKS

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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PB_TetON_mCherry-P2A

Plasmid

#238238

Purpose

For integration of mCherry control

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-P2A

Plasmid

#238231

Purpose

For overexpression of mCherry control

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_V5-EGFP-NPM1

Plasmid

#238264

Purpose

For overexpression of V5-EGFP-NPM1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

V5-EGFP-NPM1

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-SURF6

Plasmid

#238252

Purpose

For overexpression of mCherry-SURF6

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-SURF6

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_V5-nb-KS-FtoG

Plasmid

#238267

Purpose

For overexpression of V5-nb-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

V5-nb-KS-FtoG

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_TagBFP-P2A

Plasmid

#238272

Purpose

For overexpression of TagBFP-control

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

TagBFP

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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CT362

Plasmid

#233679

Purpose

Red light-inducible Cre-C; Cre-230C-PhyA

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

Cre-230C-PhyA

Expression

Mammalian

Mutation

None

Promoter

CAG

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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CT611

Plasmid

#233669

Purpose

Red light-inducible Cre-C; Cre-230C-FHY1

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

Cre-230C-FHY1

Use

Cre/Lox

Expression

Mammalian

Mutation

None

Promoter

CAGG

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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