We narrowed to 4,210 results for: Pet2

1. Met1 diUb (1-152)

   Plasmid

   #110766

   Purpose

   Bacterial expression of Met1 diUb

   Depositor

   David Komander

   Article

   Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

   Insert

   UBB (UBB Human)
   

   Use

   Tags

   N-His6-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. His6-EGFP-PH-PLCD1

   Plasmid

   #183675

   Purpose

   PtdIns(4,5)P2 biosensor for purification from bacteria

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   PLCD1 (PLCD1 Human)
   

   Use

   Tags

   His6-EGFP

   Expression

   Bacterial

   Mutation

   Amino acids 11-140

   Promoter

   T7

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3I91

   Plasmid

   #62514

   Purpose

   Bacterial expression plasmid for use in structural determination.

   Depositor

   Cheryl Arrowsmith

   Article

   Kaustov et al J Biol Chem. 2011 Jan 7;286(1):521-9. do

   Insert

   CBX8 (CBX8 Human)
   

   Use

   Tags

   6xHis and TEV cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pBJG1 T7-8xHis-OGT 5N5A

   Plasmid

   #154280

   Purpose

   Bacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

   Depositor

   Suzanne Walker

   Article

   Levine et al J Am Chem Soc. 2018 Mar 14;140(10):3510-

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Tags

   8x His, HRV3C protease site, and T7 leader

   Expression

   Bacterial

   Mutation

   N322A, N356A, N390A, N424A, N458A mutations; bact…

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-dSpCas9-HF1-VP64-6xHis

   Plasmid

   #92118

   Purpose

   Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A, N497A, R661A, Q695A, H840A, Q926A

   Promoter

   T7

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. His6tev CBP BCHZDeltaAL

   Plasmid

   #99551

   Purpose

   Bacterial expression of His tagged mouse CBP HAT Brd-ZZ(1079-1751) 1557-1617 replaced by SGGSG

   Depositor

   Peter Wright

   Article

   Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

   Insert

   mouse CBP BRD-ZZ domains (1079-1751) DeltaAL (Crebbp Mouse)
   

   Use

   Tags

   His6tev

   Expression

   Bacterial

   Mutation

   replaced 1557-1618 with SGGSG

   Promoter

   T7

   Available since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Pdpy-30-miniCAFE

   Plasmid

   #170115

   Purpose

   Dpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Insert

   MiniCAFE
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D8A, HNH-truncation (Δ495–609 aa) in CjCas9

   Promoter

   Dpy-30

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. His6-SMT3-SopB

   Plasmid

   #183676

   Purpose

   Recombinant Salmonella Typhimurium SopB

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium, Budding Yeast)
   

   Use

   Tags

   His6-S. cerevisiae SMT3(2-101)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSN672

   Plasmid

   #177362

   Purpose

   Expresses human KIF1A(1-393) fused with leucine zipper and His tag

   Depositor

   Shinsuke Niwa

   Article

   Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

   Insert

   KIF1A (KIF1A Human)
   

   Use

   Tags

   His tag and Leucine zipper

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. His-GINA3

    Plasmid

    #74980

    Purpose

    Expresses His tagged GINA3 protein in bacteria.

    Depositor

    Pradipta Ghosh

    Article

    Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71

    Insert

    Rattus guanine nucleotide binding protein alpha inhibiting 3 (Gnai3) (Gnai3 )
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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