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AMIGO2-CD4d3+4-bio

Plasmid

#73103

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding AMIGO2 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

AMIGO2 (AMIGO2 Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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EF1a_MYC deltaMBII_P2A_Hygro_Barcode

Plasmid

#120505

Purpose

Barcoded lentiviral vector to express c-MYC deltaMBII in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

c-MYC deltaMBII (MYC Human)

Use

Lentiviral

Mutation

Deletion of MYC Box II domain

Promoter

EF1a

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ORAI1-YFP V107M

Plasmid

#114180

Purpose

ORAI1 channel with C-terminal YFP, carrying the mutation V107M in the protein 1st transmembrane domain, responsible for a constitutive activity and associated with tubular aggregate myopathy (TAM).

Depositor

Nicolas Demaurex

Article

Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

Insert

ORAI1-YFP V107M (ORAI1 Human)

Tags

YFP

Expression

Mammalian

Mutation

V107M

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF138-WT

Plasmid

#78920

Purpose

Mammalian expression of RNF138 with an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

Insert

RNF138 (RNF138 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pICSL11060

Plasmid

#68264

Purpose

Level 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100)-IRES-puromycin

Plasmid

#223965

Purpose

Dual fluorescent reporter for histone H2B and for Cdt1

Depositor

Hee Won Yang

Article

Zhang et al Nat Commun. 2023 Nov 29;14(1):7847. doi:

Insert

H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100) (H2B, CDT1 Human)

Use

Lentiviral

Tags

iRFP670 and mCerulean

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Hes1

Plasmid

#61534

Purpose

Expresses Hes1 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Hes1 (Hes1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-Ago2

Plasmid

#74231

Purpose

GFP-Ago2 lentiviral vector

Depositor

Carl Novina

Article

Janas et al RNA. 2012 Nov;18(11):2041-55. doi: 10.12

Insert

Ago2 (AGO2 Human)

Use

Lentiviral

Tags

eGFP

Available Since

April 6, 2016

Availability

Academic Institutions and Nonprofits only
LLP773_pLenti-6xHRBKET-sgRNA

Plasmid

#211766

Purpose

Multiplexed gRNA plasmid targeting six different gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1), with mCherry selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

gRNAs against six different human gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1)

Use

Lentiviral

Promoter

pCMV and U6

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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Zebrafish aBb crystallin 2kb promoter/AcGFP

Plasmid

#98099

Purpose

Zebrafish aBb crystallin 2kb promoter segment driving GFP expression

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha Bb-crystallin promoter 2 kb fragment, Danio rerio (cryabb Zebrafish)

Use

Gfp expressing

Tags

GFP

Promoter

Danio alpha Bb-crystallin 2 kb fragment (-2092/-1)

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_HAND2_P2A_Hygro_Barcode

Plasmid

#120447

Purpose

Barcoded lentiviral vector to express HAND2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HAND2 (HAND2 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-OsTIR1(WT)

Plasmid

#140729

Purpose

OsTIR1(WT)-P2A-mAID-EGFP-NES

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(WT)-P2A-mAID-EGFP-NES

Use

AAV

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-fDIO-GRAB_eCB2.0

Plasmid

#164610

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in FLP positive cells

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Use

AAV

Mutation

S383T

Promoter

EFS

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST SNAP29-V5

Plasmid

#69821

Purpose

Expresses SNAP29-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Synaptosomal-associated protein 29 (SNAP29 Human)

Use

Retroviral

Tags

V5

Expression

Mammalian

Promoter

cmv

Available Since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-HP1α-miRFP670-Cry2WT

Plasmid

#122442

Purpose

Expresses disordered protein HP1α fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

HP1 alpha (CBX5 Human)

Use

Lentiviral

Tags

Cry2WT and miRFP670

Expression

Mammalian

Promoter

SFFV

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-MEF2CA

Plasmid

#61538

Purpose

Expresses MEF2CA in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

MEF2CA (MEF2C Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
EF1a_KLF4_P2A_Hygro_Barcode

Plasmid

#120455

Purpose

Barcoded lentiviral vector to express KLF4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF4 (KLF4 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAG876 pDisplay-pFAST

Plasmid

#172868

Purpose

expresses pFAST at the surface of mammalian cells

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

pFAST-PDGFR

Tags

IgK leader sequence (N terminal on insert) and My…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Blast-sgNT

Plasmid

#138678

Purpose

Expresses a Non-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgNT

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_eCB2.0mut

Plasmid

#164607

Purpose

Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in Cre positive neurons

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid non-responsive sensor GRAB_eCBmut

Use

AAV

Mutation

S383T, F177A

Promoter

hSyn

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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