We narrowed to 10,964 results for: T7

Showing: 8241 - 8260 of 10964 results

Results per page:

Sort results by:

pET41a iGluSnFR E25R

Plasmid

#119831

Purpose

Production of recombinant iGluSnFR E25R variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR E25R

Tags

GST

Expression

Bacterial

Mutation

E25R

Promoter

T7

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
N229A Lo-MetQ

Plasmid

#118268

Purpose

N229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structure

Depositor

Douglas Rees

Article

Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

Insert

MetQ (N229A) (metQ E. coli)

Tags

10x His Tag and Enterokinase Cleavage Site

Expression

Bacterial

Mutation

Changed Asparagine (Asn) 229 to Alanine (Ala)

Promoter

T7 Promoter

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTG-mTDGa.0

Plasmid

#81048

Purpose

bacterial expression of murine TDG

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TDG (Tdg Mouse)

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(P46A)

Plasmid

#112721

Purpose

This mutation (P46A) marks the first residue within the evolved region of U1A.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P46A)

Tags

6His-TEV

Expression

Bacterial

Mutation

P46A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(Q48T)

Plasmid

#112725

Purpose

This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48T)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48T

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(P51A)

Plasmid

#112728

Purpose

Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P51A)

Tags

6His-TEV

Expression

Bacterial

Mutation

P51A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(Q54A)

Plasmid

#112730

Purpose

Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q54A)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q54A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-30a(+)-C-SH2

Plasmid

#111419

Purpose

express murine Syk (C)SH2 domain (His 162 to Gln 264), in E.coli strain Rosetta 2 (DE3)

Depositor

Carol Post

Article

Feng et al Protein Sci. 2018 Jul 27. doi: 10.1002/p

Insert

murine Syk (C)SH2 domain (His 162 to Gln 264), without any tag (Syk Mouse)

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-30a(+)-N-SH2

Plasmid

#111274

Purpose

express murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)

Depositor

Carol Post

Article

Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

Insert

murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)

Tags

HHHHHHENLYFQG

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a - Ec-ClpX D144L

Plasmid

#111514

Purpose

Expresses E. coli ClpX with a C-terminal His tag and a mutation from D144 to L144

Depositor

Judy Lieberman

Article

Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

Insert

ClpX

Tags

His tag

Expression

Bacterial

Mutation

Aspartatic acid 144 to Leucine

Promoter

T7

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET22b-ecDHFR-dCys-T46C

Plasmid

#110543

Purpose

Bacterial expression of E.coli DHFR C85A/C152S/T46C mutant

Depositor

Stephen Benkovic

Article

Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

Insert

ecDHFR

Expression

Bacterial

Mutation

T46 mutated to C46, C85 mutated to A85, C152 muta…

Promoter

T7

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET22b-ecDHFR-dCys-L54C

Plasmid

#110542

Purpose

Bacterial expression of E.coli DHFR C85A/C152S/L54C mutant

Depositor

Stephen Benkovic

Article

Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

Insert

ecDHFR

Expression

Bacterial

Mutation

L54 mutated to C54, C85 mutated to A85, C152 muta…

Promoter

T7

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET22b-ecDHFR-D27S/Y100F

Plasmid

#110827

Purpose

Bacterial expression of E.coli DHFR D27S/Y100F mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

Insert

ecDHFR

Expression

Bacterial

Mutation

D27 mutated to S27, Y100 mutated to F100

Promoter

T7

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-PDAE

Plasmid

#110180

Purpose

Expresses propanediol dehydratase activating enzyme in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase Activating Enzyme

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b-PD

Plasmid

#110168

Purpose

Expresses propanediol dehydratase in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase

Tags

His6 and S-tag

Expression

Bacterial

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJSC176 - Bacterial expression plasmid for SpCas9 + Q926A variant

Plasmid

#101206

Purpose

Bacterial expression plasmid for SpCas9 + Q926A variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant Q926A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

Q926A

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
RGD-I27-CC

Plasmid

#85393

Purpose

MTFM mechanosensor for integrin. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines for immobilization and site for p-azidophenylalanine incorporation & Cy3 labeling

Depositor

Khalid Salaita

Article

Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:

Insert

RGD motif for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon

Tags

6x His

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET151-GFP-PP-RPN2 (918-953)

Plasmid

#73743

Purpose

Expression of GFP-PP-RPN2 (918-53) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 918-953 of RPN2

Promoter

T7

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pET151-GFP-PP-RPN2 (932-953)

Plasmid

#73745

Purpose

Expression of GFP-PP-RPN2 (932-953) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 932-953 of RPN2

Promoter

T7

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pET151-GFP-PP-RPN2 (918-947)

Plasmid

#73747

Purpose

Expression of GFP-PP-RPN2 (918-947) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 918-947 of RPN2

Promoter

T7

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only