We narrowed to 9,959 results for: CAG

Showing: 8261 - 8280 of 9959 results

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3xMS2 TR knockin sgRNA 1

Plasmid

#207607

Purpose

sgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

Depositor

Jens Schmidt

Publication

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

cgactcgcccggcagcgcac

Tags

None

Expression

Mammalian

Promoter

CMV and U6

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD2 N-terminal sgRNA

Plasmid

#207098

Purpose

pX330 based plasmid for expression of Cas9 and the TTTTTATCAGAAATCATGAG sgRNA to target the SHLD2 locus.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTTTTATCAGAAATCATGAG

Expression

Mammalian

Promoter

CMV and U6

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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MDC1 N-terminal sgRNA

Plasmid

#207090

Purpose

pX330 based plasmid for expression of Cas9 and the GTATCCTTCCCAGATCATGG sgRNA to target the MDC1 locus.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GTATCCTTCCCAGATCATGG

Expression

Mammalian

Promoter

CMV and U6

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pDY279

Plasmid

#182959

Purpose

Str resistant,CoE1* ori. CRISPR/Cas9 gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations in ptsI. (for 1st round editing)

Depositor

Shelley Copley

Publication

Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

Insert

gRNA (ttcaggcattttagcatccc), homologous repair template for ptsI

Use

Synthetic Biology

Expression

Bacterial

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX458-dErbB3

Plasmid

#197356

Purpose

A knock-out vector for dog ErbB3.

Depositor

Michiyuki Matsuda

Publication

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

A gRNA targeting the dog ERBB3 gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Expression

Mammalian

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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HSF2 KO

Plasmid

#200208

Purpose

gRNA for HSF2 KO

Depositor

Sheila Teves

Publication

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

HSF2 KO (Hsf2 Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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MDM4_Deletion_Downstream_gRNA_2

Plasmid

#195137

Purpose

gRNA in a third generation gRNA backbone with mCherry, targeting region immediately downstream of MDM4, to be used with MDM4_Deletion_Upstream_gRNA_1/2 for MDM4 deletion

Depositor

Jason Sheltzer

Publication

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 Deletion Downstream gRNA 2 (MDM4 Human)

Expression

Mammalian

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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MDM4_Deletion_Downstream_gRNA_1

Plasmid

#195136

Purpose

gRNA in a third generation gRNA backbone with mCherry, targeting region immediately downstream of MDM4, to be used with MDM4_Deletion_Upstream_gRNA_1/2 for MDM4 deletion

Depositor

Jason Sheltzer

Publication

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 Deletion Downstream gRNA 1 (MDM4 Human)

Expression

Mammalian

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCK411.RR1

Plasmid

#192644

Purpose

Expresses sgRNA RR1 (target mRFP CDS) on ColE1-AmpR

Depositor

Jesse Zalatan

Publication

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Insert

BBa_J23119-RR1

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23119

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shSrpk3-1

Plasmid

#180400

Purpose

Producing AAV that encodes mouse Srpk3 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Publication

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shSrpk3-1 (Srpk3 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shSrpk3-2

Plasmid

#180401

Purpose

Producing AAV that encodes mouse Srpk3 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Publication

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shSrpk3-2 (Srpk3 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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hu6-sgCebpd_v2 (opti)

Plasmid

#177250

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpd gRNA

Depositor

Monte Winslow

Publication

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpd_2nd

Use

Lentiviral

Promoter

hU6

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hU6-sgLkb1_IW (opti)

Plasmid

#177243

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Lkb1 gRNA

Depositor

Monte Winslow

Publication

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgLkb1_v1

Use

Lentiviral

Promoter

hU6

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pT-GFP-rG2

Plasmid

#188968

Purpose

IPTG inducible GFP with sgRNA

Depositor

Seung-Goo Lee

Publication

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

GFP
sgRNA: agtccatgtaatcagcgtctactagt

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptrc

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_MBD1_MBD-R22A

Plasmid

#179405

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD(R22A)

Depositor

Tuncay Baubec

Publication

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MBD1_MBD(R22A)

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

MBD R22A

Promoter

CAGGS

Available since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 FDX1-2

Plasmid

#184477

Purpose

Lentivirus gRNA targeting human FDX1 gene

Depositor

Todd Golub, Peter Tsvetkov

Publication

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

FDX1 (FDX1 Human)

Use

Lentiviral

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-IAP-del-gRNA2

Plasmid

#185003

Purpose

expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

Depositor

Denes Hnisz

Publication

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

IAP-del-gRNA2 (Akp3 Mouse)

Use

CRISPR

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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Eomes gRNA#2

Plasmid

#170835

Purpose

Cas9-mediated knockout of Eomes in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Eomes (Eomes Mouse)

Use

CRISPR

Expression

Mammalian

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Eomes gRNA#3

Plasmid

#170836

Purpose

Cas9-mediated knockout of Eomes in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Eomes (Eomes Mouse)

Use

CRISPR

Expression

Mammalian

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-mfn2 guide

Plasmid

#121993

Purpose

An entry vector with U6a and U6c promoter driving mfn2 guide RNAs expression

Depositor

Qing Deng

Publication

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

mfn2 gRNA

Use

CRISPR

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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