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pMpGWB301

Plasmid

#68629

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Publication

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMZ376

Plasmid

#74213

Purpose

Combined sgRNA/Cas9 vector with KanMX marker

Depositor

Mikel Zaratiegui

Inserts

Cas9
rrk1:sgRNA

Use

CRISPR

Expression

Yeast

Mutation

Silent muation of the CspCI site

Promoter

adh1 and rrk1

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 PC

Plasmid

#67132

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Publication

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

PC (PC Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pAM1414

Plasmid

#40237

Depositor

Susan Golden

Publication

Andersson et al Methods Enzymol. 2000;305:527-42.

Insert

promoterless luxAB reporter

Use

Cyanobacteria cloning vector

Promoter

n/a

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pICH41421

Plasmid

#50339

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, nos (A. tumefaciens)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pEN_TmiR_Luc-A

Plasmid

#25760

Purpose

Entry vector withTRE promoter driving control Luciferase miR30-based shRNA.

Depositor

Iain Fraser

Publication

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Luciferase miR-shRNA

Use

Gateway Cloning

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pMax_mOrange_G299C

Plasmid

#177829

Purpose

Site-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).

Depositor

Zachary Nagel, Leona Samson

Publication

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mOrange_G299C

Expression

Mammalian

Mutation

G299C

Promoter

CMV

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Cre-p2a-tdTomato

Plasmid

#176837

Purpose

Express Cre in mammalian cells and enable FACS sorting Cre expressing cells by tdTomato expression

Depositor

Rudolf Jaenisch

Publication

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Cre-P2A-tdTomato

Expression

Mammalian

Promoter

CAGGS

Available since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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6x-His_A1aY1_GFP

Plasmid

#158753

Purpose

Bacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purification

Depositor

Minhajuddin Sirajuddin

Publication

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

6xHistidine_A1aY1_GFP

Tags

6x-Histidine tag, GFP, T7 leader sequence, and Th…

Expression

Bacterial

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-ITSN1

Plasmid

#129614

Purpose

RhoGEF

Depositor

Dorus Gadella

Publication

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

ITSN1

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB451

Plasmid

#74833

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCB6-X:31HA

Plasmid

#74371

Purpose

Mammalian expression of wild-type HA from the X:31 strain of influenza virus

Depositor

Judith White

Publication

Qiao et al Mol Biol Cell. 1999 Aug;10(8):2759-69.

Insert

HA

Expression

Mammalian

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
PGL3Basic-Mdm2-T1

Plasmid

#32365

Depositor

Hong Wu

Publication

Chang et al J Biol Chem. 2004 Jul 9;279(28):29841-8.

Insert

Mdm2 promoter (Mdm2 Mouse)

Expression

Mammalian

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pENTR4_TAF15

Plasmid

#26368

Depositor

Thomas Tuschl

Publication

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Insert

TAF15 (TAF15 Human)

Use

Gateway Cloning

Available since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-Tspan4

Plasmid

#210323

Purpose

Expresses Tetraspanin-4 (TSPAN4) as migrasome marker

Depositor

Li Yu

Publication

Ma et al Cell Res. 2015 Jan;25(1):24-38. doi: 10.

Insert

Tetraspanin 4 (Tspan4 Mouse)

Tags

GFP

Expression

Mammalian

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pJB06

Plasmid

#190480

Purpose

Base Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis system

Depositor

Joseph Sorg

Publication

Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

Inserts

Cas9
XylR

Use

E. coli / b. subtilis - c. difficile shuttle vect…

Promoter

xylR-driven promoter

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBTK622

Plasmid

#180592

Purpose

tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

Depositor

Jeffrey Barrick

Publication

Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

Inserts

tdk
kanR

Use

Synthetic Biology

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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PARG-c002

Plasmid

#173107

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PARG (PARG Human)

Tags

mCerulean

Expression

Bacterial

Mutation

S448-T976

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR221_SLC9A3

Plasmid

#132234

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC9A3 (SLC9A3 Human)

Expression

Mammalian

Available since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pN-PITCh-HBTH

Plasmid

#127883

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HBTH tagging

Depositor

Peter Kaiser

Publication

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-HBTH

Use

CRISPR

Expression

Mammalian

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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