We narrowed to 11,712 results for: NSI

1. pGEX-THTAP NRBF2 MIT residues 1-111

   Plasmid

   #180621

   Purpose

   Bacterial expression for NRBF2 MIT domain, residues 1-111. Has N-terminal HIS-GST. Internal ID: WISP20-88.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   NRBF2 (NRBF2 Human)
   

   Tags

   HIS-GST

   Expression

   Bacterial

   Mutation

   Residues 1-111

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-sgCtrl_EF1a-Puro-T2A-BFP

   Plasmid

   #195500

   Purpose

   Lentiviral BFP expression vector bearing a sgRNA targeting a randomized human TSS sequence

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP, Puro, and T2A

   Expression

   Mammalian

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-sgT-REX17_EF1a-Puro-T2A-BFP

   Plasmid

   #195501

   Purpose

   Lentiviral BFP expression vector bearing a sgRNA targeting the promoter of human T-REX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP, Puro, and T2A

   Expression

   Mammalian

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC1-ND-Cdt1-mCitrine

   Plasmid

   #193771

   Purpose

   Human Cdt1 (SV40 NLS localization and C-terminal mCitrine tag) with mutations which abroggate its S phase degradation

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   ND-Cdt1-mCitrine (CDT1 Human)
   

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

   Promoter

   CMV

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIFEX-A2aGH

   Plasmid

   #160542

   Purpose

   pITY4 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

   Depositor

   Michelle O'Malley

   Article

   Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

   Insert

   Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
   

   Expression

   Yeast

   Mutation

   N/A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEFEX1-A2aGH

   Plasmid

   #160543

   Purpose

   pYES2 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

   Depositor

   Michelle O'Malley

   Article

   Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

   Insert

   Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
   

   Expression

   Yeast

   Mutation

   N/A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEFEX2-A2aGH

   Plasmid

   #160544

   Purpose

   pYES2 derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

   Depositor

   Michelle O'Malley

   Article

   Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

   Insert

   PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
   

   Expression

   Yeast

   Mutation

   N/A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEFEX3-A2aGH

   Plasmid

   #160545

   Purpose

   pYES2 derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

   Depositor

   Michelle O'Malley

   Article

   Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

   Insert

   PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
   

   Expression

   Yeast

   Mutation

   N/A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCFEX1-A2aGH

   Plasmid

   #160546

   Purpose

   pYC2/CT derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

   Depositor

   Michelle O'Malley

   Article

   Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

   Insert

   Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
   

   Expression

   Yeast

   Mutation

   N/A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCFEX2-A2aGH

    Plasmid

    #160547

    Purpose

    pYC2/CT derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

    Depositor

    Michelle O'Malley

    Article

    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

    Insert

    PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
    

    Expression

    Yeast

    Mutation

    N/A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCFEX3-A2aGH

    Plasmid

    #160548

    Purpose

    pYC2/CT derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

    Depositor

    Michelle O'Malley

    Article

    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

    Insert

    PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
    

    Expression

    Yeast

    Mutation

    N/A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCSmEn2ERT(#117)

    Plasmid

    #184079

    Purpose

    cyclofen-inducible chicken EN2 (wt) activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    6his-myc-chkEn2-ERT2
    

    Use

    Synthetic Biology

    Tags

    6his-Myc

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available Since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCSm5En2CER4(#457)

    Plasmid

    #184090

    Purpose

    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    5myc-chkEn2(C>S)-ERT2
    

    Use

    Synthetic Biology

    Tags

    5xMyc

    Expression

    Mammalian

    Mutation

    chkEn2: C175S; ERT2: G400V, M543A, L544A, deleted…

    Promoter

    sCMV+SP6

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT22UbGfpnE4PCh(#301)

    Plasmid

    #184066

    Purpose

    cyclofen-inducible GFP nuclear relocation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Zhang et al Chembiochem. 2018 Jun 18;19(12):1232-123

    Insert

    eGFP-nls-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223-ZNF532

    Plasmid

    #88740

    Purpose

    Donor Vector containing ZNF532 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF532 (ZNF532 Human)
    

    Use

    Gateway donor vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

16. pDONR223-ZNF77

    Plasmid

    #88744

    Purpose

    Donor Vector containing ZNF77 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF77 (ZNF77 Human)
    

    Use

    Gateway donor vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

17. pDONR223-ZNF672

    Plasmid

    #88753

    Purpose

    Donor Vector containing ZNF672 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF672 (ZNF672 Human)
    

    Use

    Gateway donor vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

18. pDONR223-ZNF678

    Plasmid

    #88761

    Purpose

    Donor Vector containing ZNF678 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF678 (ZNF678 Human)
    

    Use

    Gateway donor vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

19. pDONR223-ZNF688_isoform1

    Plasmid

    #88779

    Purpose

    Donor Vector containing ZNF688 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF688 (ZNF688 Human)
    

    Use

    Gateway donor vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

20. pDONR223-ZNF688_isoform2

    Plasmid

    #88783

    Purpose

    Donor Vector containing ZNF688 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF688 (ZNF688 Human)
    

    Use

    Gateway donor vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only