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Plasmid#109358PurposeMammalian vector for expressing effector domains fused to dCas9.DepositorTypeEmpty backboneUseCRISPRTagsdCas9, SV40 NLS, 3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only
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pAAV-SMVP-Cas9N-U6-gRNA M3
Plasmid#80936PurposeExpresses Cas9N in mammalian cells; expresses gRNA M3 for Mstn cleavage.DepositorInsertCas9N
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC-(loxP2-attB2-SA(0)-T2A-Gal4-Hsp70)
Plasmid#62954PurposeSinglet donor (phase 0) for in vivo RMCE with MiMIC inserts to make Gal4 driver.DepositorInsertT2A-Gal4-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter
Plasmid#61762PurposeDual Color betaGlobin reporter to study splicing kinetics in vivoDepositorInsertb-Globin
UseTags24 X MS2 hairpin cassette in 3'UTR of b-Glob…ExpressionMammalianMutationPromoterDox inducible Tet-ON promoterAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC-(loxP2-attB2-SA(1)-T2A-Gal4-Hsp70)
Plasmid#62955PurposeSinglet donor (phase 1) for in vivo RMCE with MiMIC inserts to make Gal4 driver.DepositorInsertT2A-Gal4-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-ATL3-deltaTM
Plasmid#109025Purposestable lentiviral expression of cDNADepositorInsertATL3-deltaTM (ATL3 Human)
UseLentiviralTagseGFPExpressionMammalianMutationtruncated form without C-terminal ER transmembran…PromoterEIF-1a promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDule- Mj Acd A9
Plasmid#197651PurposeE. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pairDepositorInsertsMj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA
UseTagsNoneExpressionBacterialMutationY32A, L65D, F108L, Q109E, D158S, L162TPromoterGlnS and lppAvailable sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-mgSrtA
Plasmid#125792PurposeTransfected into cell of interest for membrane expression. The encoded mgSrtA could enable labeling of the interacting cells which is useful for recording cell-cell interactions.DepositorInsertmgSrtA
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-itis pSel
Plasmid#195344PurposeSelection plasmid expressing non-fluorescent EGFP A111VDepositorInsertEGFP
UseCRISPRTagsExpressionMutationA111VPromoterJ23119Available sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pThpR
Plasmid#190613PurposeExpresses GFP in response to tetrahydropapaverine in E. coliDepositorInsertTHP4.1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only