We narrowed to 31,565 results for: REP

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pCXLE-hOCT3/4-shp53-F + mCherry-2A-puro

Plasmid

#74947

Purpose

Dosage control and tracing of reprogramming episomes

Depositor

Ann Zovein

Article

Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

Insert

Oct3/4 (POU5F1 Human)

Expression

Mammalian

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pOTTC592 - pCMV Mito-iRFP

Plasmid

#59135

Purpose

A plasmid that express mitochondrial-targeted iRFP under the CMV promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Mitochondria-localized Infrared Fluorescent Protein

Tags

Mitochondria-targeting sequence

Expression

Mammalian

Promoter

CMV-IE

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pOTTC590 - pCMV ER-iRFP

Plasmid

#59136

Purpose

A plasmid that express endoplasmic reticulum-targeted iRFP under the CMV promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

ER-localized Infrared Fluorescent Protein

Tags

ER retention signal and signal peptide

Expression

Mammalian

Promoter

CMV-IE

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-ATG13 (2-197)

Plasmid

#188075

Purpose

To make ATG101/ATG13 HORMA complex for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Tags

strep-strep-Flag tag

Expression

Mammalian

Mutation

ATG13 (2-197)

Promoter

CMV

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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FUGW-RUSH-LAMP1-V5-APEX2

Plasmid

#228524

Purpose

Lentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP1-V5-APEX2 in mammalian cells.

Depositor

Ginny Farias

Article

Li et al Nat Commun. 2024 Dec 30;15(1):10829. doi

Insert

LAMP1 (Lamp1 Rat)

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP), Streptavidin-…

Expression

Mammalian

Promoter

Ubc

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-RUSH-LAMP2A-mNeonGreen

Plasmid

#228525

Purpose

Lentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP2A-mNeonGreen in mammalian cells.

Depositor

Ginny Farias

Article

Li et al Nat Commun. 2024 Dec 30;15(1):10829. doi

Insert

LAMP2 (Lamp2 Rat)

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP), Streptavidin-…

Expression

Mammalian

Promoter

Ubc

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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GG-EBNA-MIR302-7guides-PGK-Puro

Plasmid

#201960

Purpose

EBNA episome plasmid for U6 promoter-driven expression of 7 gRNAs targeting miRNA302/367. Includes PGK-puro selection cassette

Depositor

Timo Otonkoski

Article

Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-

Insert

MIR302-7g-PGK-Puro

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-BI-hTFR1-3

Plasmid

#218798

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

AAV Rep-Cap BI-hTFR1-3

Expression

Mammalian

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pHX_HDR_hPAX7_IRES-EGFP-PGK-Neo

Plasmid

#160458

Purpose

Homology-directed recombination donor vector for EGFP reporter knock-in to human PAX7 3' UTR

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Inserts

PAX7 Left/5' Homology Arm (PAX7 Human)
PAX7 Right/3' Homology Arm (PAX7 Human)

Use

Insertion of a fluorescent reporter through homol…

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLD003-pCMV-APOBEC-Cas9(D10A)-rPB(8kD)

Plasmid

#165445

Purpose

Expresses ACX, rPB(8kD) in mammalian cells

Depositor

Wei Leong Chew

Article

Chen et al Nat Commun. 2021 Mar 2;12(1):1384. doi:

Insert

APOBEC-nCas9-rPB(8kD)

Expression

Mammalian

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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PCXLE-hUL + mTAGBFP2

Plasmid

#74944

Purpose

Dosage control and tracing of reprogramming episomes

Depositor

Ann Zovein

Article

Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

Insert

LMyc/Lin28 (MYCL, LIN28A Human)

Expression

Mammalian

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pTBL680 CHYRON3 integration construct

Plasmid

#126448

Purpose

To integrate the CHYRON3 locus at AAVS1 in human cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

pU6/3xLacO-CHYRON3 hgRNA
pCMV-puro

Expression

Mammalian

Mutation

The SpCas9 sgRNA constant region is mutated to ma…

Promoter

CMV and human U6/3xLacO

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC591 - pCMV Mem-iRFP

Plasmid

#59134

Purpose

A plasmid that express membrane-targeted iRFP under the CMV promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Membrane-localized Infrared Fluorescent Protein

Tags

Palmitylation signal

Expression

Mammalian

Promoter

CMV-IE

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

Plasmid

#138525

Purpose

Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.

Depositor

Richard Jenner

Article

Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

Insert

Control non-PRC2 binding A-tract repeat RNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pmx-MiGT

Plasmid

#172397

Purpose

Retroviral expression vector for murine direct cardiac reprogramming

Depositor

Li Qian

Article

Wang et al Cells. 2020 Jan 22;9(2). pii: cells90202

Insert

Mef2c-i, Gata4, Tbx5 (Gata4, Mef2c, Tbx5 Mouse)

Use

Retroviral

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract

Plasmid

#138524

Purpose

Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.

Depositor

Richard Jenner

Article

Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

Insert

PRC2-binding G-tract repeat RNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCVL.SSA TLR (CCR5 TALEN Sce Spacer)

Plasmid

#46941

Purpose

Codes for the SSA-TLR with the target site for the CCR5 TALEN with the spacer region corresponding to I-Sce I in a lentiviral backbone

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Inserts

5' iRFP arm
eGFP with TALEN TS
+3 mCherry
3' iRFP arm

Use

Lentiviral

Expression

Bacterial and Mammalian

Mutation

embedded TALEN TS from 163-218, truncated 25 amin…

Promoter

SFFV

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pRP[2CRISPR]-mCherry/Hygro-hCas9-U6>{mdx left}-U6>{mdx right}

Plasmid

#184381

Purpose

This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

Depositor

Ori Bar-Nur

Article

Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

Insert

Cas9, two gRNAs targeting dystrophin

Use

Lentiviral

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pTBL681 CHYRON4 integration construct

Plasmid

#126449

Purpose

To integrate the CHYRON4 locus at AAVS1 in human cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

pU6/3xLacO-CHYRON4 hgRNA
pCMV-puro

Expression

Mammalian

Mutation

The SpCas9 sgRNA constant region is mutated to ma…

Promoter

CMV and human U6/3xLacO

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF12_P2A_Hygro_Barcode

Plasmid

#120498

Purpose

Barcoded lentiviral vector to express KLF12 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF12 (KLF12 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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