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ecAT3.10

Plasmid

#107215

Purpose

Mammalian expression plasmid to target the ecAT3.10 extracellular ATP sensor to the cell surface.

Depositor

Mathew Tantama

Article

Conley et al PLoS One. 2017 Nov 9;12(11):e0187481. do

Insert

ecAT3.10

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pXN21-Dest

Plasmid

#105086

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

Depositor

Christopher Grefen

Article

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Tags

NubG

Expression

Yeast

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-mRuby2

Plasmid

#104058

Purpose

AAV expression of Cre-dependent reporter mRuby2

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

mRuby2

Use

AAV

Promoter

CAG

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pEF5-FRT-V5-DEST-GFP-Kif26b

Plasmid

#102862

Purpose

Expresses GFP-Kif26b in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Mouse Kif26b (Kif26b Mouse)

Tags

GFP

Expression

Mammalian

Promoter

EF1A

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-PANX1(HCVp)

Plasmid

#87698

Purpose

Express HCV protease-activatible pannexin-1 in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PANX(HCVp)

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf6(WT)-mCherry

Plasmid

#79422

Purpose

Expresses C-terminally mCherry-tagged Arf6(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf6 (ARF6 Human)

Tags

mCherry

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCuo DN Rac1

Plasmid

#84644

Purpose

dominant negative Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.

Depositor

Sanjay Kumar

Article

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

Rac1 (RAC1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Dominant Negative

Promoter

cumate-inducible promoter

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only
SIVu

Plasmid

#81089

Purpose

Single Integration Vector URA - Cloning vector for single integration into the URA3 locus of S.cerevisiae containing two MCS.

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Delta(Pro-MP)ADAM17-HA

Plasmid

#65221

Purpose

Expression of HA tagged ADAM17 lacking prodomain and metalloprotease domain in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

Delta(Pro-MP)ADAM17 (ADAM17 Human)

Tags

HA

Expression

Mammalian

Mutation

deleted AA 18-473

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-KIF1B beta-FL

Plasmid

#79108

Purpose

Mammalian expression of GFP-KIF1B beta-FL

Depositor

Susanne Schlisio

Article

Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

Insert

GFP-KIF1B beta full length (KIF1B Human)

Tags

GFP

Expression

Mammalian

Mutation

exon 25 missing (natural splice varient)

Promoter

CMV

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
pMSCV-FLAG-hHelios-IRES-GFP

Plasmid

#74047

Purpose

retroviral plasmid for expression of FLAG-tagged human Helios (IKZF2) corresponding to cDNA from NM_001079526.1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human Helios (IKZF2) (IKZF2 )

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

encodes shorter version of human Helios

Promoter

pMSCV-LTRs

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti.CMV/TO_PRKAA2

Plasmid

#74447

Purpose

Lentiviral expression vector encoding untagged WT human AMPK alpha 2

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

AMPK alpha 2 (PRKAA2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT/TO-Flag-RNF169

Plasmid

#74243

Purpose

Expresses RNF169 in mammalian cells

Depositor

Daniel Durocher

Article

Panier et al Mol Cell. 2012 Aug 10;47(3):383-95. doi:

Insert

RNF169 (RNF169 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pCZGY2849

Plasmid

#74112

Purpose

Enhanced mutant version of membrane targeted PH-miniSOG (Q103L) entry clone for more efficient ablation of cells when expressed under desired promoter.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

miniSOG(Q103L)

Use

Gateway topo cloning

Tags

PH

Available Since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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pNatA (pACYCduet-naa10-naa15)

Plasmid

#72928

Purpose

Allows expression of a modified version of the fission yeast NatA complex - chloramphenicol marker

Depositor

Dan Mulvihill

Article

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Inserts

naa10 (naa10 Synthetic, Fission Yeast)
naa15 (naa15 Fission Yeast)

Expression

Bacterial

Mutation

Codon optomised for E.coli expression

Promoter

T7

Available Since

Feb. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-R2505P

Plasmid

#69015

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-R2505P (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Arginine 2505 to Proline

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-I2500F

Plasmid

#69014

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-I2500F (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Isoleucine 2500 to Phenylalanine

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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p2,0_3E5E_luciferase

Plasmid

#69358

Purpose

Encodes for Ebola Minigenome with luciferase reporter gene under control of a T7 RNA polymerase promoter

Depositor

Elke Mühlberger

Article

Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

Insert

3E5E luciferase minigenome

Use

T7

Promoter

T7

Available Since

Nov. 30, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCXLE-dCas9VP192-T2A-EGFP

Plasmid

#69536

Purpose

Episomal plasmid encoding dCas9VP192

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

dCas9-dCas9VP192-EGFP

Use

CRISPR

Tags

EGFP

Promoter

CAG

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pPS22 (pET23a-Cas10/Csm/csm2H6N)

Plasmid

#70038

Purpose

Bacterial expression of the S. epidermidis Cas10·Csm complex

Depositor

Luciano Marraffini

Article

Hatoum-Aslan et al J Biol Chem. 2013 Sep 27;288(39):27888-9

Insert

Cas10/Csm complex

Tags

6xHIS on N terminus of Csm2

Expression

Bacterial

Promoter

T7

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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