We narrowed to 9,182 results for: Pol

1. pCAG-T3-st1CAS9-pA

   Plasmid

   #131542

   Purpose

   Expresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii

   Article

   Fujii et al Biochem Biophys Res Commun. 2016 Jun 16.

   Insert

   Codon optimized st1Cas9
   

   Use

   CRISPR

   Tags

   Flag, NLS, and polyA

   Expression

   Mammalian

   Available Since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOCC167

   Plasmid

   #118909

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C protease

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC8-TwinStrep-3C-DrHDAC6 (40-831)

   Plasmid

   #180226

   Purpose

   Baculovirus transfer plasmid to express TwinStrep-3C-DrHDAC6 (40-831)

   Depositor

   Patrick Matthias

   Article

   Langousis et al Methods Mol Biol. 2023;2589:467-480. doi

   Insert

   Histone deacetylase 6 (hdac6 Zebrafish)
   

   Tags

   TwinStrep-3C

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRJPaph-mTq2

   Plasmid

   #67031

   Purpose

   Plasmid for Paph-mTurqoise2 integration downstream of B. diazoefficiens (B. japonicum) scoI

   Depositor

   Julia Vorholt

   Article

   Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

   Inserts

   B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
   mTurqoise2
   

   Use

   Conjugative bacterial vector

   Available Since

   Oct. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOCC193

   Plasmid

   #118903

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP, cleavable with 3C and monomeric GFP and C-terminal TwinStrep, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
   

   Tags

   MBP, cleavable with 3C protease; mGFP and TwinStr…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSBL_DK193

   Plasmid

   #156424

   Purpose

   Insect cell expression of 3a(Q57H)-sfGFP, needs to be introduced into baculoviral bacmid

   Depositor

   Stephen Brohawn

   Article

   Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

   Insert

   SARS-CoV-2 3a protein (Q57H)
   

   Tags

   sfGFP-His

   Expression

   Insect

   Mutation

   Q57H

   Promoter

   Polyhedrin

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV6M-Pak1 P13A

   Plasmid

   #12207

   Depositor

   Jonathan Chernoff

   Article

   Galisteo et al J Biol Chem. 1996 Aug 30. 271(35):20997-

   Insert

   Pak1 (PAK1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   P13A: disrupts first polyproline region of Pak1

   Available Since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOCC93

   Plasmid

   #118911

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with GP64 viral secretion signal and C-terminal eGFP-HIS6

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-GP64ss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
   

   Tags

   eGFP-HIS6

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS5

   Plasmid

   #55750

   Purpose

   Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

   Depositor

   Matthew Bennett

   Article

   Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

   Insert

   KanR
   

   Expression

   Bacterial

   Mutation

   Residues 59-264 of KanR

   Promoter

   T7-lac

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. GST-SmB'

    Plasmid

    #1842

    Depositor

    Philip Leder

    Article

    Bedford et al Proc Natl Acad Sci U S A 1998 Sep 1;95(1

    Insert

    SmB' (SNRPB Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pBTK550d

    Plasmid

    #110617

    Purpose

    BTK assembled broad-host-range plasmid encoding inducible GFP driven by T7 polymerase

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    Aug. 10, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pJet-CMV-CD5HA2-bglpA

    Plasmid

    #96962

    Purpose

    Expression plasmid for HA-tag embedded into human CD52 with short polyA

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    CMV-CD5HA2-bglpA
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. TIA1 RRM-CFP-FRBx5

    Plasmid

    #103780

    Purpose

    expresses iPOLYMER component in mammalian cells

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    TIA1 RRM-CFP-FRBx5
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOCC121

    Plasmid

    #118893

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and monomeric GFP and C-terminal HIS6, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and MBP, cleavab…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOCC70

    Plasmid

    #118878

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette
    

    Tags

    mGFP, cleavable with TEV protease; HIS6, cleavabl…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUWR-CadTS

    Plasmid

    #58325

    Purpose

    Destination vector for inserting ubi-CadTS to Drosophila melanogaster genome

    Depositor

    Denise Montell

    Article

    Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

    Insert

    E-cadherin tension sensor
    

    Expression

    Insect

    Promoter

    poly-ubiquitin

    Available Since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pRJPaph-HcRed

    Plasmid

    #67035

    Purpose

    Plasmid for Paph-HcRed integration downstream of B. diazoefficiens (B. japonicum) scoI

    Depositor

    Julia Vorholt

    Article

    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

    Inserts

    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    HcRed
    

    Use

    Conjugative bacterial vector

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJEC626

    Plasmid

    #174367

    Purpose

    Activation domain for modular CRISPRa. AD-SYNZIP. Expresses alphaNTD from the E coli RNAP with a SYNZIP17 domain fused to the N terminus.

    Depositor

    James Chappell

    Article

    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

    Insert

    Alpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23106

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pK RagA-RagC

    Plasmid

    #99649

    Purpose

    Bacterial expression of wildtype Rag GTPase dimer

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    10x His tag, Poly arginine linker, and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. DPAGT1 (DPAGT1A-c005)

    Plasmid

    #98228

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    DPAGT1A (DPAGT1 Human)
    

    Use

    Baculovirus

    Tags

    His-6-TEV

    Mutation

    pfam00953:Glycos_transf_4 98-275

    Promoter

    polyhedrin

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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