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pRSF_his6_sumo_SARS_CoV2_NSP14_P335S

Plasmid

#213524

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation P335S

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed P335 to S

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_P335H

Plasmid

#213525

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation P335H

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed P335 to H

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_H424R

Plasmid

#213526

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation H424R

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed H424 to R

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pYX-N-Puro-mNeon-TSC22D1

Plasmid

#222667

Purpose

endogenously tag TSC22D1 with N-terminal mNeon

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

TSC22D1 (TSC22D1 Human)

Tags

mNeon

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pYX-N-Blast-miniTurbo-TSC22D2

Plasmid

#222669

Purpose

endogenously tag TSC22D2 with N-terminal miniTurbo

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

TSC22D2 (TSC22D2 Human)

Tags

miniTurbo

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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CILK1-R272Q

Plasmid

#214911

Purpose

expression of the mutant variant of CILK1

Depositor

Pavel Krejčí

Article

Paige Taylor et al Hum Mol Genet. 2016 Sep 15;25(18):3998-4

Insert

CILK1 (CILK1 Human)

Tags

Myc-FLAG

Expression

Mammalian

Mutation

R272Q substitution

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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CILK1-E80K

Plasmid

#214910

Purpose

expression of the mutant variant of CILK1

Depositor

Pavel Krejčí

Article

Paige Taylor et al Hum Mol Genet. 2016 Sep 15;25(18):3998-4

Insert

CILK1 (CILK1 Human)

Tags

Myc-FLAG

Expression

Mammalian

Mutation

E80K substitution

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-748_HA-GD2-28z_CAR_BATF

Plasmid

#207488

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

BATF, HA-GD2-28z_CAR (BATF Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-805_HA-GD2-28z_CAR_BATF-RFP

Plasmid

#207492

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

BATF-RFP, HA-GD2-28z_CAR (BATF Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-755_HA-GD2-28z_CAR_RFP-JUN

Plasmid

#207494

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

RFP-JUN, HA-GD2-28z_CAR (JUN Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-837_NY-ESO-1_TCR_tFAS

Plasmid

#207500

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

tFAS, NY-ESO-1_TCR (FAS Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-CMV-Cre-U6-sgNotch2#1-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

Plasmid

#209069

Purpose

Entry vector that encodes sgRNAs against mouse Notch2, Rb1, Trp53, Rbl2, and CMV Cre recombinase.

Depositor

Matthew Oser

Article

Hong et al Cancer Res. 2022 Jan 15;82(2):248-263. d

Insert

sgRNAs targeting Notch2, Rb1, Trp53, Rbl2 and Cre recombinase

Use

Gateway vector to be used for lr reaction

Promoter

U6

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-CMV-Cre-U6-sgNotch1#1-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

Plasmid

#209068

Purpose

Entry vector that encodes sgRNAs against mouse Notch1, Rb1, Trp53, Rbl2, and CMV Cre recombinase.

Depositor

Matthew Oser

Article

Hong et al Cancer Res. 2022 Jan 15;82(2):248-263. d

Insert

sgRNAs targeting Notch1, Rb1, Trp53, Rbl2 and Cre recombinase

Use

Gateway vector to be used for lr reaction

Promoter

U6

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-CSRNP2-P2A-mCherry

Plasmid

#203867

Purpose

Tet-inducible lentiviral plasmid expressing CSRNP2-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

CSRNP2 (CSRNP2 Human)

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Myc-Bnip3-T181A

Plasmid

#197556

Purpose

Mammalian expression of myc-tagged mouse Bnip3 with an alanine substitution of threonine-181. Based on Myc-Bnip3FL (#100796)

Depositor

Joseph Gordon

Article

Martens et al Cell Death Dis. 2021 Nov 26;12(12):1105.

Insert

BCL2 Interacting Protein 3 (Bnip3 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

Threonine 181 changed to alanine

Promoter

CMV

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pIG-720 CTLA4-GFP R75W

Plasmid

#186125

Purpose

CTLA4 gene knockin with mutation

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CTLA4 (CTLA4 Human)

Use

CRISPR

Mutation

R75W

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9-2A-GFP-sgRNA-RDH11

Plasmid

#161924

Purpose

To generate RDH11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against RDH11 exon 2.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

sgRNA targeting RDH11 exon 2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Y478C-WLS-HA-IRES-GFP

Plasmid

#178063

Purpose

Expression of WLS-HA protein (Y478C mutant)

Depositor

Joseph Gleeson

Article

Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

Insert

WLS (WLS Human)

Tags

HA and IRES-GFP

Expression

Mammalian

Mutation

Y478C

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Y392C-WLS-HA-IRES-GFP

Plasmid

#178062

Purpose

Expression of WLS-HA protein (Y392C mutant)

Depositor

Joseph Gleeson

Article

Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

Insert

WLS (WLS Human)

Tags

HA and IRES-GFP

Expression

Mammalian

Mutation

Y392C

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-I531T-WLS-HA-IRES-GFP

Plasmid

#178064

Purpose

Expression of WLS-HA protein (I531T mutant)

Depositor

Joseph Gleeson

Article

Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

Insert

WLS (WLS Human)

Tags

HA and IRES-GFP

Expression

Mammalian

Mutation

I531T

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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