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We narrowed to 14,449 results for: eng

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Showing: 8361 - 8380 of 14449 results

  2. PASTA-3_pUC19_HDR-Pa01-attG_TRAC_for-Cas12a

    Plasmid
    #247039
    Purpose
    Encodes HDR-template for Cas12a-mediated Knock-In of size-reduced Pa01-attG (landing pad) into TRAC gene (exon1). Template region to be PCR-amplified.
    Depositor
    Dimitrios Wagner
    Publication
    Kassing et al bioRxiv 2025.09.10.675267
    Insert
    Pa01 bacterial attachment site (attB) = genomic attachment site (attG), flanked by human TRAC homology arms
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    247039-plasmid-map-sequence-id-499186

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  3. PASTA-5_pUC19_IVT-ePa01

    Plasmid
    #247041
    Purpose
    Plasmid for in vitro transcription (IVT) of enhanced Pa01 (ePa01) mRNA. Use indicated PCR-primers for correction of T7-mutation and addition of poly-A-tail.
    Depositor
    Dimitrios Wagner
    Publication
    Kassing et al bioRxiv 2025.09.10.675267
    Insert
    ePa01 recombinase (large serine integrase, LSR) for IVT
    Expression
    Bacterial and Mammalian
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    247041-plasmid-map-sequence-id-500291

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  4. TP1376

    Plasmid
    #252368
    Purpose
    Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag
    Depositor
    Tino Pleiner
    Publication
    Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.
    Insert
    anti-EMC Nanobody G9
    Tags
    ALFA-GFP-SUMOEu and His10
    Expression
    Bacterial
    Mutation
    Contains engineered nanobody variant with ALFA ta…
    Available since
    April 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    252368-plasmid-map-sequence-id-504517

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  5. pX461-ABE8e-nSpCas9

    Plasmid
    #237460
    Purpose
    Transfection-based all-in-one base editor plasmid that expresses both adenine base editor (ABE8e-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Publication
    Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:
    Insert
    3xFlag_ABE8e_SpCas9(D10A)_6xNLS
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    chicken beta-actin promoter & U6
    Available since
    Jan. 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    237460-plasmid-map-sequence-id-476159

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  6. pX461-evoCDA1-nSpCas9

    Plasmid
    #237461
    Purpose
    Transfection-based all-in-one base editor plasmid that expresses both cytosine base editor (evoCDA1-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Publication
    Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:
    Insert
    3xFlag_evoCDA1_ssDBD_nickase SpCas9 _6xUGI
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    chicken beta-actin promoter & U6
    Available since
    Jan. 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    237461-plasmid-map-sequence-id-476160

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  7. pLENTICRISPR V2 sgCGAS_1

    Plasmid
    #241454
    Purpose
    Delete CGAS
    Depositor
    Richard Possemato
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgCGAS_1 (CGAS Synthetic)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Nov. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    241454-plasmid-map-sequence-id-492099

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  8. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

    Plasmid
    #223137
    Purpose
    pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223137-plasmid-map-sequence-id-447707

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  10. pcDNA3.1-PDGFRA-V5/HIS

    Plasmid
    #234754
    Purpose
    Expression of human PDGFRA receptor tyrosine kinase in mammalian cells
    Depositor
    Pavel Krejčí
    Publication
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PDGFRA receptor tyrosine kinase, full length, wildtype (PDGFRA Human)
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available since
    April 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    234754-plasmid-map-sequence-id-468863

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  12. MSCV-FoxP3-R337Q/A372S-IRES-Thy1.1

    Plasmid
    #229619
    Purpose
    Mammalian expression of full length mouse FoxP3 (R337Q, A372S)
    Depositor
    Sun Hur
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    full length FoxP3, carrying double mutation R337Q/A372S (Foxp3 Mouse)
    Expression
    Mammalian
    Mutation
    R337Q/A372S
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229619-plasmid-map-sequence-id-457331

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  20. kiCAP-AAV-M.Mus.1

    Plasmid
    #196681
    Purpose
    Rep/Cap plasmid for the production of M.Mus.1, an AAV capsid with CNS tropism in mice.
    Depositor
    Pardis Sabeti
    Publication
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Mutation
    WVLPSGG insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196681-plasmid-map-sequence-id-389742

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Showing: 8361 - 8380 of 14449 results