We narrowed to 5,906 results for: pcDNA 3.1

1. pcDNA3.1(+)-mCherry-p115

   Plasmid

   #181996

   Purpose

   Mammalian expression vector encoding p115 RhoGEF tagged with mCherry.

   Depositor

   Jin Zhang

   Article

   Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

   Insert

   mCherry-p115 (ARHGEF1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. NLS-4LNDDE488Q-pCDNA3.1(+)

   Plasmid

   #172314

   Purpose

   Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)

   Depositor

   Joshua Rosenthal

   Article

   Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:

   Insert

   Lambda N peptide - human ADAR2 Deaminase Domain E488Q
   

   Use

   Tags

   6xHis, Flag, and Nuclear Localization Signal of S…

   Expression

   Mammalian

   Mutation

   changed the Glutamic acid 488 in the Deaminase Do…

   Promoter

   CMV

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-MS2-HA-HshnRNPE2iso3_Z

   Plasmid

   #148118

   Purpose

   Mammalian Expression of HshnRNPE2iso3

   Depositor

   Elisa Izaurralde

   Insert

   HshnRNPE2iso3 (PCBP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one non silent mutation L239 to S compapred to th…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1_GEM4-Halo-FRB

   Plasmid

   #197057

   Purpose

   Expression of Paraburkholderia xenovorans encapsulin Halo-FRB-fusion in mammalian cells

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   Encapsulin
   

   Use

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1_GEM7-Halo-FRB

   Plasmid

   #197058

   Purpose

   Expression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cells

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   Encapsulin
   

   Use

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1_GEM22-Halo-FRB

   Plasmid

   #197059

   Purpose

   Expression of Mycobacterium hassiacum encapsulin Halo-FRB-fusion in mammalian cells

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   Encapsulin
   

   Use

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1(+)- e18 LOF

   Plasmid

   #131729

   Purpose

   Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")

   Depositor

   Alberto Ciccia

   Article

   Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

   Insert

   e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1_FRB-GEM23-Halo

   Plasmid

   #197060

   Purpose

   Expression of nanocage I3-01 Halo-FRB-fusion in mammalian cells

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   I3-01
   

   Use

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. h-Plekhm1_FLAG-pcDNA 3.1

   Plasmid

   #73593

   Purpose

   Express human Plekhm1 in mammalian cells

   Depositor

   Paul Odgren

   Article

   Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

   Insert

   Plekhm1 (PLEKHM1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CrBlue-mcherry - pcDNA3.1

    Plasmid

    #64204

    Purpose

    CrBlue fused to mcherry on the C terminus

    Depositor

    Narasimhan Gautam

    Article

    Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

    Insert

    CrBlue
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1+ Flag-RAP2A

    Plasmid

    #128099

    Purpose

    Expresses RAP2A in mammalian cells in a G418-selectable plasmid

    Depositor

    Chi Van Dang

    Article

    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-3xFLAG-RSL1D1(WT)

    Plasmid

    #122301

    Purpose

    expresses RSL1D1 protein with a flag tag

    Depositor

    Gerardo Ferbeyre

    Article

    Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

    Insert

    RSL1D1 (RSL1D1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1 Flag-Sesn2

    Plasmid

    #61868

    Purpose

    Transient overexpression of Flag-Sesn2 (mouse)

    Depositor

    Ming Li

    Article

    Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

    Insert

    Sesn2 (Sesn2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1-CnA-H151A

    Plasmid

    #191956

    Purpose

    Expression of phosphatase dead mutant of calcineurin A (CnA-H151A).

    Depositor

    Luca Scorrano

    Insert

    Calcineurin subunit alpha H151A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H151A

    Promoter

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-GoldenGate-VP64

    Plasmid

    #47389

    Purpose

    Expression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning system

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

    Type

    Empty backbone

    Use

    TALEN

    Tags

    FLAG, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-ccdB-Firefly

    Plasmid

    #87066

    Purpose

    Mammalian Gateway-compatible expression vector backbone with a C-terminal Firefly luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Use

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1 HA-rnSOX9 ΔC (1-381)

    Plasmid

    #62973

    Purpose

    Expresses HA-tagged rat SOX9 residues 1-383

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    SOX9 (Sox9 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    codes for residues 1-381

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1-MAP3K5 (R256C)

    Plasmid

    #47105

    Purpose

    Expresses mutant (R256C) human MAP3K5 in mammalian cells

    Depositor

    Yardena Samuels

    Insert

    MAP3K5 (MAP3K5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed arginine 256 to cysteine

    Promoter

    CMV

    Available since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

19. CRT-GFP pcDNA3.1

    Plasmid

    #80978

    Purpose

    mammalian expression of CRT-GFP

    Depositor

    Peter Steyger

    Article

    Karasawa et al Toxicol Sci. 2011 Dec;124(2):378-87. doi

    Insert

    CRT (Calr Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    no KDEL

    Promoter

    CMV

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1 myc-PITPbeta

    Plasmid

    #58278

    Purpose

    mammalian expression of rat PITP beta

    Depositor

    Shamshad Cockcroft

    Insert

    PITP beta (Pitpnb Rat)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only