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Plasmid#179559Purposeencodes S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoterDepositorInsertUseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only
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GCN5 FL-dCas9-CBP core
Plasmid#179556Purposeencodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoterDepositorInsertUseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
GCN5 core-dCas9-CBP core
Plasmid#179555Purposeencodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoterDepositorInsertUseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pInducer-myc-ERK1-R84S/D338N
Plasmid#213594PurposeDoxycycline inducible expression of myc-tagged ERK1-R84S/D338N cDNA in mammilian cellsDepositorInsertMAPK3 (MAPK3 Human)
UseLentiviralTagsMYCExpressionMammalianMutationR84S, D338NPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInducer-myc-ERK1-R84S/S170D/D338N
Plasmid#213595PurposeDoxycycline inducible expression of myc-tagged ERK1-R84S/S170D/D338N cDNA in mammilian cellsDepositorInsertMAPK3 (MAPK3 Human)
UseLentiviralTagsMYCExpressionMammalianMutationR84S, S170D, D338NPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInducer-myc-ERK1-R84S/S170D/Y280A
Plasmid#213596PurposeDoxycycline inducible expression of myc-tagged ERK1-R84S/S170D/Y280A cDNA in mammilian cellsDepositorInsertMAPK3 (MAPK3 Human)
UseLentiviralTagsMYCExpressionMammalianMutationR84S, S170D, Y280APromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInducer-myc-ERK2-R67S/S153D/D321N
Plasmid#213598PurposeDoxycycline inducible expression of myc-tagged ERK2-R67S/S153D/D321N cDNA in mammilian cellsDepositorInsertMAPK1 (MAPK1 Human)
UseLentiviralTagsMYCExpressionMammalianMutationR67S, S153D, D321NPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInducer-myc-ERK2-R67S/S153D/Y263A
Plasmid#213599PurposeDoxycycline inducible expression of myc-tagged ERK2-R67S/S153D/Y263A cDNA in mammilian cellsDepositorInsertMAPK1 (MAPK1 Human)
UseLentiviralTagsMYCExpressionMammalianMutationR67S, S153D, Y263APromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CBP core-dCas9-p300 core
Plasmid#179558Purposeencodes S. pyogenes dCas9 with n-terminal fusion of human CBP core (aa 1084-1701) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoterDepositorInsertUseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
GCN5 FL-dCas9-p300 core
Plasmid#179554Purposeencodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoterDepositorInsertUseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVP315
Plasmid#222074PurposeMultiGreen 2.0 EF level 1 acceptor vector, kanamycin selection.DepositorTypeEmpty backboneUseSynthetic Biology; Greengate compatible cloning v…TagsExpressionMutationAll unused Esp3i sites are domesticatedPromoterAvailable sinceDec. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVP082
Plasmid#222034PurposeMultiGreen 2.0 EF level 1 acceptor vector, spectinomycin selection.DepositorTypeEmpty backboneUseSynthetic Biology; Greengate compatible cloning v…TagsExpressionMutationAll unused Esp3i sites are domesticatedPromoterAvailable sinceDec. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDH-EF1-Mito-pHCountdown
Plasmid#182302Purposelentiviral plasmid encoding pH-Countdown targeted to mitochondria via fusion to the COX8A presequence with puromycin selectionDepositorInsertmito pH countdown
UseLentiviralTagsCOX8A mitochondria targeting sequenceExpressionMutationPromoterEF-1aAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
EEF1A2 in pENTR
Plasmid#216585Purposegateway cloning donor vector containing EEF1A2DepositorInsertEEF1A2 (EEF1A2 Human)
UseGateway entry plasmidTagsExpressionMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSELECT-HA-mFOXO1-D256
Plasmid#83379Purposeexpresses mouse FOXO1 with truncating mutation at AA 256 in mammalian cellsDepositorInsertForkhead box O1 (Foxo1 Mouse)
UseTagsHAExpressionMammalianMutationN256TAG (premature stop codon)PromoterEF-1aAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ad-EEF1D-S133E
Plasmid#131577PurposeExpresses EEF1D with S133E mutation in mammalian cellsDepositorInsertEukaryotic translation elongation factor 1 delta (EEF1D Human)
UseAdenoviralTagsNoExpressionMutationchanged Serine 133 to Glutamic acidPromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
BAP1-wt (siRNA resistant)
Plasmid#108439PurposeStable expression of BAP1-wt in mammalian cells, gene has an introduced siRNA-resistance against siBAP1.DepositorInsertBAP1 (BAP1 Human)
UseTagsExpressionMammalianMutationc.1641C>T, c.1644T>A, c.1647T>C, c.1650C…PromoterCMVAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
GCN5 core-dCas9-p300 core
Plasmid#179553Purposeencodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoterDepositorInsertUseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-UbC-LARGE1-EF1a-BSD
Plasmid#205151PurposeExpress UbC-driven LARGE1 and EF-1a driven BSDDepositorInsertLARGE xylosyl- and glucuronyltransferase 1 (LARGE1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterUbCAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only