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pRC319

Plasmid

#61272

Purpose

Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.

Depositor

Timothy Lu

Article

Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

Insert

Cas9, tracrRNA, crRNA

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD24-fis

Plasmid

#51557

Purpose

E. coli fis ORF cloned into pBAD24 expression vector

Depositor

Francisco Malagon, Sankar Adhya

Article

Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

Insert

FIS (fis E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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pMM733

Plasmid

#68898

Purpose

IPTG-inducible CRISPRi vector targeting NanoLuc (NL4), constiutive NanoLuc, pNBU2 backbone, AmpR

Depositor

Christopher Voigt, Timothy Lu

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA
NanoLuc

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pET14-Encap

Plasmid

#86405

Purpose

Expresses T. maritima encapsulin in E. coli

Depositor

David Savage

Article

Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

Insert

Encapsulin

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV_dCas9_VP64

Plasmid

#49015

Purpose

encodes human-optimized dCas9_VP64 synthetic transcription factor

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

dCas9_VP64 (human-codon-optimized)

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST co hPHGDH

Plasmid

#154915

Purpose

Express codon optimized human PHGDH

Depositor

Richard Possemato

Article

Richard et al Richard Possemato Lab plasmids

Insert

PHGDH, codon optimized (PHGDH Human, Synthetic)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Codon optimized for expression in human cells

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMV306G13+FFluc

Plasmid

#26157

Depositor

Brian Robertson, Siouxsie Wiles

Article

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

G13 promoter + Firefly luciferase

Use

Mycobacteria integrating vector

Tags

Expression

Mutation

Codon optimized for Mycobacterium tuberculosis. …

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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MG-155

Plasmid

#26529

Depositor

David Baltimore

Article

O'Connell et al J Exp Med. 2008 Mar 17. 205(3):585-94.

Insert

miR-155 (MIR155 Human)

Use

Retroviral

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pMPMA3dPlac-PssaG-GFPLVA

Plasmid

#23343

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology. 2009 Dec 24. ():.

Insert

GFP(LVA)

Use

Tags

GFP(LVA)

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2010

Availability

Academic Institutions and Nonprofits only
pMM710

Plasmid

#68893

Purpose

IPTG-inducible CRISPRi vector targeting nonsense sequence (NS), pNBU2 backbone, AmpR

Depositor

Christopher Voigt, Timothy Lu

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-mScarlet-H-mNeonGreen-kanMX (pKBJ009)

Plasmid

#173456

Purpose

Contains mScarletH-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-H-mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-mCherry-mNeonGreen-kanMX (pKBJ006)

Plasmid

#173453

Purpose

Contains mCherry-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMPMA3dPlac-PfliC-GFPLVA

Plasmid

#23342

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology. 2009 Dec 24. ():.

Insert

GFP(LVA)

Use

Tags

GFP(LVA)

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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NFkBm2

Plasmid

#15090

Depositor

David Baltimore

Article

Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

miR-146a promoter

Use

Tags

Expression

Mammalian

Mutation

2 NFkB binding sites mutated

Promoter

Available since

June 6, 2007

Availability

Academic Institutions and Nonprofits only
pMM731

Plasmid

#68896

Purpose

IPTG-inducible CRISPRi vector targeting NanoLuc (NL1), constiutive NanoLuc, pNBU2 backbone, AmpR

Depositor

Christopher Voigt, Timothy Lu

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA
NanoLuc

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pRS41K-mCherry-sfGFP (pJJF001)

Plasmid

#173457

Purpose

C-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-sfGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMPMA3dPlac-PsopB-GFPLVA

Plasmid

#23341

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology. 2009 Dec 24. ():.

Insert

GFP(LVA)

Use

Tags

GFP(LVA)

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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pMM763

Plasmid

#68900

Purpose

IPTG-inducible CRISPRi vector targeting PcfiA (PR1), constiutive NanoLuc, pNBU2 backbone, AmpR

Depositor

Christopher Voigt, Timothy Lu

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA
NanoLuc

Use

Tags

Expression

Mutation

G1104E

Promoter

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pSopBWT

Plasmid

#112512

Purpose

PsopB driven sopB and sigE

Depositor

Olivia Steele-Mortimer

Article

Knodler et al Cell Microbiol. 2009 Nov;11(11):1652-70.

Insert

sopB/sigE operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

PsopB

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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mRFP1-36

Plasmid

#63825

Purpose

A member of an RBS library for mRFP1 with different translation rates

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

mRFP1

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

J23100

Available since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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