We narrowed to 28,296 results for: cmv promoter

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pST1374-N-NLS-flag-linker-Cas9-D10A

Plasmid

#51130

Purpose

Cas9 nickase optimized for nuclear import.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Insert

Cas9 nickase

Use

CRISPR

Tags

Flag and NLS

Expression

Mammalian

Mutation

D10A (nickase mutant)

Promoter

CMV

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Drp1K38A

Plasmid

#45161

Depositor

Alexander van der Bliek, Richard Youle

Article

Smirnova et al J Cell Biol. 1998 Oct 19;143(2):351-8.

Insert

Drp1 (DNM1L Human)

Tags

HA (AYPYDVPDY)

Expression

Mammalian

Mutation

K38A

Promoter

CMV

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pmCherry-CRY2-CreN

Plasmid

#26888

Purpose

Expresses N-terminal fragment of Cre fused to CRY2 for blue light-induced activation of DNA recombination when used with CIBN-CreC. Also expresses mCherry.

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CRY2-CreN (cre, CRY2 Mustard Weed)

Expression

Mammalian

Mutation

aa 19-104 of Cre

Promoter

CMV

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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4xmts-mScarlet-I

Plasmid

#98818

Purpose

In vivo visualization of mitochondria (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

mts

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mNeptune2-N

Plasmid

#41645

Purpose

Expresses far-red FP mNeptune2 fused to Dronpa145N. Dronpa145N oligomerizes under violet light (390-400nm) and dissociates under cyan light (490-500nm)

Depositor

Michael Lin

Article

Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

Insert

mNeptune2-Dronpa145N

Expression

Mammalian

Mutation

K145N on Dronpa (allows control of photochromic F…

Promoter

CMV

Available Since

Dec. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pJL-SaKKH-BE3

Plasmid

#85170

Purpose

Expresses SaKKH-BE3 in mammalian cells [Edit]

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

SaKKH-BE3

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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Str-Golgin84_VSVG-SBP-EGFP

Plasmid

#65305

Purpose

synchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Golgin-84 (GOLGA5 Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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moxGFP

Plasmid

#68070

Purpose

C-terminal fusion to moxGFP

Depositor

Erik Snapp

Article

Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

Type

Empty backbone

Tags

moxGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pTriEx4-Rac1-2G

Plasmid

#66110

Purpose

Second generation Rac1 FRET biosensor

Depositor

Olivier Pertz

Article

Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

Insert

Second generation Rac1 FRET biosensor

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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DT041A

Plasmid

#159402

Purpose

Constitutive murine TrkB expression in mammalian host, controlled under cytomegalovirus (CMV) promoter and enhancer

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pLenti-CMV-TrkB (Ntrk2, Mouse) (Ntrk2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV promoter

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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p3259 pCMV hUBC9 mt HA

Plasmid

#14439

Depositor

Peter Howley

Article

Yasugi et al Nucleic Acids Res. 1996 Jun 1. 24(11):20

Insert

UBC9 C93S (UBE2I Human)

Tags

HA

Expression

Mammalian

Mutation

Cys93Ser mutation (TGC changed to AGC)

Promoter

CMV

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pNEU-hMbPylRS-4xU6M15

Plasmid

#105830

Purpose

Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells

Depositor

Irene Coin

Article

Serfling et al Nucleic Acids Res. 2017 Nov 21. pii: 464

Inserts

Humanized MbPylRS
tRNAM15

Expression

Mammalian

Mutation

(Y271A, Y349F)MbPylRS

Promoter

CMV and U6

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pmScarlet_alphaTubulin_C1

Plasmid

#85045

Purpose

In vivo visualization of microtubules (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Alpha-Tubulin (TUBA1B Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-BRD2

Plasmid

#65376

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD2 (BRD2 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pmGFP-P2A-K0-P2A-RFP

Plasmid

#105686

Purpose

Double fluorescent stalling reporter K0

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K0
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(Hygro) myc-hPolQ-Flag

Plasmid

#73132

Purpose

To express in mammalian cells human PolQ

Depositor

Agnel Sfeir

Article

Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

Insert

Polymerase theta (POLQ Human)

Tags

Flag and myc

Expression

Mammalian

Promoter

cmv promoter

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer

Plasmid

#41584

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Tags

Flag

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3 IKKe flag

Plasmid

#26201

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

I-kappa-B kinase epsilon (IKBKE Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pLX302_FOXA1-V5

Plasmid

#70090

Purpose

Expression of human FOXA1 with C-terminal V5 tag, puromycin selection marker, 3rd generation lentiviral vector

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCDH-puro-myr-HA-Akt1

Plasmid

#46969

Purpose

lentiviral expression of myr-HA-Akt1

Depositor

Jialiang Wang

Article

Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

Insert

AKT1 (AKT1 Human)

Use

Lentiviral

Tags

HA and myr

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only

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