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pX330A-Prdm14-L4-#2

Plasmid

#171512

Purpose

deletion of a genomic locus in Prdm14 gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

Prdm14 (Prdm14 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pPD127 AND Gate, 2 repeats

Plasmid

#138936

Purpose

EYFP reporter vector for the Logic 37 AND 158 NOT 43 2 repeats promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

Logic 37 AND 158 NOT 43 2 repeats

Use

Synthetic Biology

Expression

Mammalian

Promoter

Logic 37 AND 158 NOT 43 2 repeats

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Sce-Invertase 2 SP

Plasmid

#153493

Purpose

S. cerevisiae Invertase Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Invertase 2 SP

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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GAL-MAGE-2-429

Plasmid

#89126

Purpose

mammalian expression of full-length human MAGE-A11 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

MAGE-A11 (MAGEA11 Human)

Tags

GAL4 DBD

Expression

Mammalian

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
VP-MAGE-2-429

Plasmid

#89125

Purpose

mammalian expression of full-length human MAGE-A11 with N-terminal VP16 activation domain

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

MAGE-A11 (MAGEA11 Human)

Tags

VP16

Expression

Mammalian

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
pLL3.7 Alk.2 shRNA

Plasmid

#59297

Purpose

shRNA to mouse Alk

Depositor

Ulrike Heberlein

Article

Lasek et al J Neurosci. 2011 Oct 5;31(40):14134-41.

Insert

Alk.2 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
human MMP-2 promoter

Plasmid

#53432

Purpose

promoter activity study

Depositor

Etty Benveniste

Article

Qin et al J Biol Chem. 1999 Oct 8;274(41):29130-7.

Insert

MMP2 promoter (MMP2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

MMP2

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

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Lsg-GBA-1 + 2

Plasmid

#199276

Purpose

nicking sgRNA to induce or correct GBA (c. 1226 A > G) mutation using PE3

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

GBA nick

Expression

Mammalian

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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3336 pcDNA3 Bcl-2

Plasmid

#8768

Depositor

Stanley Korsmeyer

Article

Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

Insert

Bcl-2 (BCL2 Human)

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pBS mouse IRS-2

Plasmid

#11372

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-2 (Irs2 Mouse)

Expression

Bacterial

Available Since

March 1, 2006

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.GFP.miR-218-2-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85881

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-218-2-3p Sensor (MIR218-2 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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p2Flag CMV2-ZO-2

Plasmid

#27415

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Tags

2xFlag

Expression

Mammalian

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only
pFYF1327 EGFP Site#2

Plasmid

#47512

Purpose

human gRNA expression vector targeting EGFP

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-EGFP site 2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pLG018 (AVAST type 2)

Plasmid

#157896

Purpose

Expresses the AVAST type 2 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

AVAST type 2 (STAND ATPase with uncharacterized helical N-terminal domain)

Expression

Bacterial

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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E47 2/5-pCS2

Plasmid

#20914

Depositor

Stephen Tapscott

Article

Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

Insert

E47 2/5 (TCF3 Human)

Expression

Mammalian

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pCMV-NHERF1-PDZ1-2

Plasmid

#28294

Depositor

Maria-Magdalena Georgescu

Article

Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

Insert

NHERF1 (NHERF1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains aa 1-298 (deletion of the EB domain aa 2…

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
GLI-3 bs-2

Plasmid

#16420

Depositor

Bert Vogelstein

Article

Kinzler et al Nature. 1988 Mar 24. 332(6162):371-4.

Insert

GLI3 (GLI3 Human)

Expression

Bacterial

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
Fluorescent reporter for MOBE1-2

Plasmid

#219945

Purpose

Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE1-2

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-3'end

Use

CRISPR

Expression

Mammalian

Mutation

EGFP(A111V/L202S)

Promoter

CMV, U6

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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1/2-NORAD-(A)4x

Plasmid

#199208

Purpose

1/2 truncation of NORAD mRNA tagged with 4 copies of Riboglow (variant A)

Depositor

Esther Braselmann

Article

Sarfraz et al Nat Commun. 2023 Feb 16;14(1):867. doi:

Insert

1/2-NORAD

Tags

Riboglow RNA tag

Expression

Mammalian

Mutation

4 copies of Riboglow tag after stop codon

Promoter

CMV

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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ZE45 Dvl-2 pCS2P+

Plasmid

#17086

Depositor

Randall Moon

Article

Waxman et al Development. 2004 Dec. 131(23):5909-21.

Insert

Dvl-2 (dvl2 Zebrafish)

Use

Zebrafish expression

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only