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ISA431: aTc-MP6

Plasmid

#189935

Purpose

Mutagenesis Plasmid: pTet, danQ926, dam, seqA, emrR, ugi and cda1, tetR, CloDF13, KanR

Depositor

Nathan Crook

Article

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

danQ926, dam, seqA, emrR, ugi and cda1

Expression

Bacterial

Promoter

pTet

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pKCyR8

Plasmid

#149494

Purpose

Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Hygromycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CymR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFLxR3

Plasmid

#167518

Purpose

Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Tetracycline Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSB1K3_M13ps

Plasmid

#190434

Purpose

M13 packaging signal cloned into the pSB1K3 vector.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

M13 packaging signal

Use

Synthetic Biology

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pQLinkG2

Plasmid

#13671

Depositor

Konrad Buessow

Article

Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

Insert

multi cloning site

Use

Co-expression

Tags

GST, TEV, and attB1

Expression

Bacterial

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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pFCiR2

Plasmid

#167512

Purpose

Broad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, mRFP, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CinRAM-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCin

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pGE144 (MutaEco; aTC-induced)

Plasmid

#189824

Purpose

Expresses MutaEco for generating mutations in the E. coli genome (aTC-induced)

Depositor

Seokhee Kim

Article

Eom et al Nucleic Acids Res. 2022 Apr 22;50(7):e38

Insert

EcoRNAPα-PmCDA1

Expression

Bacterial

Promoter

tetR/tetA promoter

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pBTG2

Plasmid

#149475

Purpose

Broad-Host range expression vector, pBBR origin, TetR repressor, PTetA promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

TetR-GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTetA

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTXB1-AbaSFI

Plasmid

#86900

Purpose

Express CBD and intein tag containing AbaSI enzyme full length

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

AbaSDFI

Tags

CBD

Promoter

T7

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pFCyR2

Plasmid

#167513

Purpose

Broad-Host range expression vector, RSF1010 origin, CymRAM repressor, PCymRC promoter, mRFP, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CymRAM-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pBLxG2

Plasmid

#149472

Purpose

Broad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKCyR6

Plasmid

#149493

Purpose

Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Spectinomycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CymR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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R5 PV

Plasmid

#51734

Purpose

Dicistronic vector with PV IRES

Depositor

Vincent Mauro

Article

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Insert

PV IRES

Promoter

SV40

Available Since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

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pFCiKm7

Plasmid

#167521

Purpose

Broad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, KmR, Erythromycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CinRAM-KmR

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCin

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pTC-0X1L

Plasmid

#20315

Depositor

Dirk Schnappinger

Article

Klotzsche et al Nucleic Acids Res. 2009 Apr;37(6):1778-8

Insert

Pmyc1tetO-lacZ (lacZ E. coli)

Expression

Bacterial

Available Since

March 4, 2009

Availability

Academic Institutions and Nonprofits only

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pJBL3907

Plasmid

#193140

Purpose

Clostridium beijerinckii pfl ZTP riboswitch with sfGFP reporter

Depositor

Julius Lucks

Article

Bushhouse et al Nucleic Acids Res. 2023 Mar 2:gkad110. d

Insert

Clostridium beijerinckii (Cbe) pfl ZTP riboswitch with sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pKLxR5

Plasmid

#149465

Purpose

Broad-Host range expression vector, RK2 and pUC origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBRG2

Plasmid

#149474

Purpose

Broad-Host range expression vector, pBBR origin, RhaR-RhaS activator, PrhaBAD promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

RhaS-RhaR-GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrhaBAD

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFVK5

Plasmid

#167522

Purpose

Broad-Host range expression vector, RSF1010 origin, VanRAM repressor, PVanCC promoter, mKelly2, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanRAM-mKelly2

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pBLxVi5

Plasmid

#167516

Purpose

Broad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, Violacein, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-Violacein

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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