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AcrIIC1-mCherry chimera #10

Plasmid

#128113

Purpose

Expresses a chimeric anti-CRISPR protein targeting the N. meningitidis Cas9

Depositor

Dominik Niopek

Article

Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

Insert

AcrIIC1-mCherry chimera #10

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pIGc-Xph15

Plasmid

#133030

Purpose

Expresses Xph15 in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph15

Tags

His10

Expression

Bacterial

Mutation

S63K

Promoter

T7

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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DYNLRB2

Plasmid

#132534

Purpose

Human Rbl-2, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLRB2 (DYNLRB2 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-Caspase-1 (C285A) -NpLxIS

Plasmid

#131354

Purpose

Expresses Caspase-1 C285A with 2 copies of pLxIS motif from STING fused to its N-ternimus

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

Caspase-1 (C285A)-NpLxIS (Casp1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

Caspase-1 (C285A)

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-V111FL114FI766F-HA-TagBFP

Plasmid

#120911

Purpose

Expresses BFP tagged Ptch1-B (V111FL114FI766F)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

HA and TagBFP

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, Y…

Promoter

CMV

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-V111FL114FW115A-HA-GFP

Plasmid

#120908

Purpose

Expresses Ptch1-B (V111FL114FW115A)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

GFP and HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-I766FV111F-HA-GFP

Plasmid

#120907

Purpose

Expresses Ptch1-B (I766FV111F)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

GFP and HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-Y233C-HA

Plasmid

#120904

Purpose

Expresses Ptch1-B (Y233C) for function assays

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, Y…

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-L234C-HA

Plasmid

#120905

Purpose

Expresses Ptch1-B (L234C)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, L…

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pSN28

Plasmid

#100113

Purpose

expresses a chimeric cMYC-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCerulean-P2A-cMYC fused to a 'weak' VP16 domain (MYC Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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