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pRetroX-TRE3G-IRSp53 P278D P281D

Plasmid

#221320

Purpose

Dox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 (Baiap2 Mouse)

Use

Retroviral

Tags

3xFLAG

Mutation

P278D P281D

Promoter

TRE3G

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-IRSp53(trunc N1)

Plasmid

#221272

Purpose

Expression of IRSp53 N-terminal truncation 1 (257-375) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 N-terminal truncation 1 (257-375) (Baiap2 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-CNOT6

Plasmid

#221288

Purpose

Expression of CNOT6 (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

CNOT6 (pLxIS) (Cnot6 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-IRSp53(pLxIS) S269A

Plasmid

#221269

Purpose

Expression of IRSp53 pLxIS mutant in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 pLxIS (Baiap2 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Mutation

S269A

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-EGFR-L858R-T790M-dual-nick sgRNA

Plasmid

#214101

Purpose

Lentiviral vector expressing nicking sgRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two sgRNAs using independent U6 promoters.

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

EGFR L858R nicking sgRNA/EGFR T790M nicking sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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PMP34-mApple-NFAST(low)

Plasmid

#214418

Purpose

Peroxisome localization of low-affinity NFAST fused to PMP34-Apple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspA-N (SLC25A17 )

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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SpyTag003(M115G)-Talin rod-mCherry

Plasmid

#210026

Purpose

Expression of SpyTag003(M115G)-Talin rod(434-2541)-mCherry in mammalian cells

Depositor

Vesa Hytönen

Article

Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

Insert

SpyTag003(M115G)-Talin1 rod domain(434-2541)-mCherry

Expression

Mammalian

Mutation

SpyTag003 M115G mutation to modify SpyCatcher003 …

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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SpyTag003(M115A)-Talin rod-mCherry

Plasmid

#210025

Purpose

Expression of SpyTag003(M115A)-Talin rod(434-2541)-mCherry in mammalian cells

Depositor

Vesa Hytönen

Article

Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

Insert

SpyTag003(M115A)-Talin1 rod domain(434-2541)-mCherry

Expression

Mammalian

Mutation

SpyTag003 M115A mutation to modify SpyCatcher003 …

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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SpyTag003(V114T, V116T)-Talin rod-mCherry

Plasmid

#210027

Purpose

Expression of SpyTag003(V114T, V116T)-Talin rod(434-2541)-mCherry in mammalian cells

Depositor

Vesa Hytönen

Article

Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

Insert

SpyTag003(V114T,V116T)-Talin1 rod domain(434-2541)-mCherry

Expression

Mammalian

Mutation

SpyTag003 V114T and V116T mutations to modify Spy…

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-Talin head-SpyCatcher003(K31TAG)

Plasmid

#210023

Purpose

Expression of EGFP-Talin head(1-433)-SpyCatcher003(K31TAG) in mammalian cells

Depositor

Vesa Hytönen

Article

Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

Insert

EGFP-Talin head (1-433)-SpyCatcher003(K31TAG)

Expression

Mammalian

Mutation

SpyCatcher003 with K31TAG (Amber stop codon) muta…

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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