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pLenti-Hygro-sgSIK3

Plasmid

#138699

Purpose

Expresses a human SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 human (SIK3 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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Galectin3-CD4d3+4-bio

Plasmid

#73128

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Galectin3 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

LGALS3 (LGALS3 Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF11_P2A_Hygro_Barcode

Plasmid

#120497

Purpose

Barcoded lentiviral vector to express KLF11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF11 (KLF11 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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prk5-MFSD12-TEV-3xFLAG-3xHA PM mutant

Plasmid

#149507

Purpose

Transient expression of human MFSD12 with recoded nucleotide sequence. Dileucine motif LL256-257 has been mutated to AA.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

MFSD12 (MFSD12 Human)

Use

Transient expression

Tags

TEV-3XHA-3XFLAG

Expression

Mammalian

Mutation

Codon Optimized Sequence, p.LL256-257AA

Promoter

CMV

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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CIAR_pcDNA5/FRT/TO

Plasmid

#86502

Purpose

Expresses CIAR in mammalian cells. Used to generate Flp-In stables.

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

CIAR (SOS1 Human)

Use

Frt

Expression

Mammalian

Mutation

T968L in SOScat

Promoter

CMV (tet operator)

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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G718A_BattB(GA)_TKterm_AttB(GT)_sGFP-PTEN-IRES-mCherry-P2A-HygroR_noTerm_attP(GA)_IRES-UnaG-2A-Puro-Term

Plasmid

#200634

Purpose

Plasmid self-excising recombination plasmid for Matreyek Bxb1(GT) landing pad

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

PTEN (PTEN Human)

Expression

Mammalian

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-SIK1

Plasmid

#138695

Purpose

Expresses a human SIK1-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK1 human (SIK1 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Blast-sgSIK2

Plasmid

#138708

Purpose

Expresses a human SIK2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK2 human (SIK2 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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Chicken ArcLight Q175

Plasmid

#53566

Purpose

Genetically encoded voltage sensor ArcLight with improved kinetics

Depositor

Vincent Pieribone

Article

Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

Insert

Chicken ArcLight-Q175

Expression

Mammalian

Mutation

Gg-VSP contains an R153Q mutation and an amino ac…

Promoter

CMV

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Bmi1

Plasmid

#61532

Purpose

Expresses Bmi1 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Bmi1 (Bmi1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pCS3-MT-BX/p14ARF

Plasmid

#78761

Purpose

To overexpress p14ARF in Mammalian Cells

Depositor

Jaewhan Song

Article

Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

Insert

ARF (CDKN2A Human)

Tags

6xMYC

Expression

Mammalian

Promoter

CMV IE94

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only
pSc-His6-Sem1Sc

Plasmid

#83235

Purpose

pGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeast

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

Sem 1 (SEM1 Budding Yeast)

Tags

His6

Expression

Yeast

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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C1orf9-CD4d3+4-bio

Plasmid

#73109

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding C1orf9 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

C1orf9 (C1ORF9 Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-FRT-TO-Nsp7-Nsp8_GC3opt

Plasmid

#157691

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pRD395

Plasmid

#163102

Purpose

Expression of mCherry_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

mCherry_H-NSdbd

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-FUSN-miRFP670-Cry2WT

Plasmid

#122441

Purpose

Expresses disordered protein FUS(1-214) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

Cry2WT and miRFP670

Expression

Mammalian

Mutation

Contains only amino acids 1-214

Promoter

SFFV

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-V1251R_R

Plasmid

#147376

Purpose

Mammalian Expression of HsNot1iso1-V1251R

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1-V1251R (CNOT1 Human)

Expression

Mammalian

Mutation

A deletion of 5 AA 822-827 SKMKPS-> T and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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HOXB7 (human) pCMV

Plasmid

#8539

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB7 (HOXB7 Human)

Tags

FLAG

Expression

Mammalian

Mutation

starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pLKO.1-shGBP1.1.mKO2

Plasmid

#85209

Purpose

TRCN0000116119 (Target CGACGAAAGGCATGTACCATA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

GBP1 (guanylate binding protein 1) (GBP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-Flag-YAP2

Plasmid

#27472

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only