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pENTR223-BFRF3

Plasmid

#195778

Purpose

Gateway Entry Clone of Epstein-Barr virus BFRF3

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BFRF3

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BHRF1

Plasmid

#195780

Purpose

Gateway Entry Clone of Epstein-Barr virus BHRF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BHRF1 (BHRF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78306

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADfluw-Hsp70

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pBABE-Hygro HoxB9

Plasmid

#27022

Depositor

Joan Massague

Article

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

HoxB9 (HOXB9 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pLVX-UBE3BΔIQ-HA-IRES-Puro

Plasmid

#107350

Purpose

Expresses HA tagged UBE3B deletion of IQ domain in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3BdelIQ (UBE3B Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pBW313lux-hrpR

Plasmid

#61436

Purpose

pBWF2620 encoding rbs33-hrpR-ter (pBWF2620: pSB3K3 carrying BBa_F2620 (PTet-rbs34-luxR-ter))

Depositor

Martin Buck, Baojun Wang

Article

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

F2620-rbs33-hrpR-ter

Expression

Bacterial

Promoter

plux

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pRDA_791

Plasmid

#216079

Purpose

Minimal plasmid; contains only ori and ampR, destination vector

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Destination

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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AA295

Plasmid

#215986

Purpose

Fragmid fragment: (N' terminus) SV40 Nuclear Localization Signal

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; SV40NLS_v1.19

Use

Fragment

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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AA014

Plasmid

#216057

Purpose

Fragmid fragment: (2A Selection) Puromycin resistance

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

P2A_v1.1; PuroR_v1.1; Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA163

Plasmid

#216061

Purpose

Fragmid fragment: (2A Selection) Stop only

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA065

Plasmid

#215965

Purpose

Fragmid fragment: (Pol 2 promoter) human EF1a promoter

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

EF1a_v1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-hMcl-1(T163A)

Plasmid

#25376

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

hMCL1 (MCL1 Human)

Tags

V5 and His tag

Expression

Mammalian

Mutation

changed threonine 163 to alanine

Available Since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only
pCAG1.1-Myc/TSSK1

Plasmid

#199624

Purpose

Expression vector of mouse TSSK1. Myc-tag at N-terminus.

Depositor

Masahito Ikawa

Article

Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

Insert

Tssk1 mRNA (Tssk1 Mouse)

Tags

Myc-tag

Promoter

CAG

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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ATF4 11: 5’ATF4.uORF2.GFP

Plasmid

#21858

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

upstream ORF2 (uORF) of ATF4 fused to EGFP

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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AA082

Plasmid

#215997

Purpose

Fragmid fragment: (Selection) fluorescent reporter

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; mTagBFP2_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-BKRF3

Plasmid

#195790

Purpose

Gateway Entry Clone of Epstein-Barr virus BKRF3

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BKRF3

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-TSC2-NLS

Plasmid

#192441

Purpose

Encodes mCherry-tagged wild-type TSC2 isoform 5 targeted to the nucleus.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-TSC2-NLS (TSC2 Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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R26-EGFP MMEJ donor vector

Plasmid

#137926

Purpose

R0SA26 knock-in vector with microhomology-mediated end-joining(MMEJ)-based method

Depositor

Hiroshi Kiyonari

Article

Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

Insert

SA-EGFP-bpA with micro-homology arms

Use

Mouse Targeting

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-HNHx-ABEmax7.10HD-BlastR

Plasmid

#176477

Purpose

A-to-G base editor with alternative targeting scope

Depositor

Gerald Schwank

Article

Villiger et al Mol Ther Nucleic Acids. 2021 Aug 26;26:5

Insert

HNHx-ABEmax7.10HD

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR223-BGLF2

Plasmid

#195800

Purpose

Gateway Entry Clone of Epstein-Barr virus BGLF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BGLF2

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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