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pND004

Plasmid

#200950

Purpose

MBP-tagged E. coli protein expression destination vector for Golden Gate cloning

Depositor

Timothy Whitehead

Article

Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

Type

Empty backbone

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pKmK.I2R

Plasmid

#125064

Purpose

Storage plasmid for K.marxianus right homology arm

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Right homology arm for targeting integration to chromosome V: 23743..21744

Use

Synthetic Biology

Expression

Yeast

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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InterCatch-GFP

Plasmid

#186900

Purpose

Cell-free expression of transmembrane protein TfR-sfGFP-SpyCatcher-sfCherry1-10

Depositor

Taekjip Ha, Allen Liu

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

TfR-sfGFP-SpyCatcher003-sfCherry1-10

Use

Synthetic Biology

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBS Wnt-5aHA

Plasmid

#17376

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-5aHA (Wnt5a Mouse)

Tags

HA

Expression

Bacterial

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pRVV578

Plasmid

#108279

Purpose

RMCE vector containing inverted PhiC31 attB sites

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

Insert

PhiC31 attB sites

Use

CRISPR; Rmce

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pBS Wnt-4HA

Plasmid

#17375

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-4HA (Wnt4 Mouse)

Tags

HA

Expression

Bacterial

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pHAGE-TO-MCP-3XBFPnls

Plasmid

#75383

Purpose

MCP-3XBFPnls

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

MCP

Use

Lentiviral

Tags

3XBFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pJPC12-ΔM13-mCherry-PR/PRM-GFP

Plasmid

#80859

Purpose

Reporter plasmid (mCherry and GFP)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

mCherry
GFP

Expression

Bacterial

Promoter

Lambda Pr and Lambda Prm

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pKmK.I4R

Plasmid

#125066

Purpose

Storage plasmid for K.marxianus right homology arm

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Right homology arm for targeting integration to chromosome IV:240042..241741

Use

Synthetic Biology

Expression

Yeast

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pRSETB-roGFP1-R12

Plasmid

#83311

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Cannon et al Protein Sci. 2006 Jan;15(1):45-57. Epub

Insert

roGFP1-R12

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/Q80R/S147C/Q204C/N149K/F223R/S202K

Promoter

T7

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
pBS Wnt-2HA

Plasmid

#17368

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-2HA (WNT2 Human)

Tags

HA

Expression

Bacterial

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
InterTag-BFP

Plasmid

#186901

Purpose

Cell-free expression of transmembrane protein PDGFR-tagBFP-sfCherry11-SpyTag

Depositor

Taekjip Ha, Allen Liu

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

SpyTag003-sfCherry11-tagBFP-PDGFR

Use

Synthetic Biology

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBS Wnt-6HA

Plasmid

#17378

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-6HA (Wnt6 Mouse)

Tags

HA

Expression

Bacterial

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
p2lox-cAMPr R739A

Plasmid

#102618

Purpose

cAMPr with a point mutation R739A which theoretically increases its affinity for cAMP

Depositor

Justin Blau

Article

Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

Insert

cAMPr

Use

Cre/Lox

Expression

Mammalian

Mutation

R739A

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX Smad3

Plasmid

#12630

Depositor

Rik Derynck

Article

Zhang et al Nature. 1996 Sep 12. 383(6596):168-72.

Insert

Smad3 (SMAD3 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pAG504-frFAST

Plasmid

#160055

Purpose

Expresses frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

frFAST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMEG_YLCas9_empty

Plasmid

#117826

Purpose

CRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolytica

Depositor

Michael Sauer

Article

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpTEF_hCas9_YlCyc1TT

Expression

Bacterial and Yeast

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pADS

Plasmid

#19040

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

codon-optimized synthetic amorphadiene synthase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pCS4083

Plasmid

#131739

Purpose

Two Color Control Assay bicistronic BFP-mCherry-sTRSVctl

Depositor

Christina Smolke

Article

Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

Insert

sTRSV

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pECO200

Plasmid

#169765

Purpose

Express SpCas9 and Basta resistance gene

Depositor

Sang-Gyu Kim

Article

Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

Type

Empty backbone

Use

Plant binary vector

Expression

Plant

Promoter

35S, AtU6

Available Since

July 16, 2021

Availability

Academic Institutions and Nonprofits only

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