We narrowed to 13,995 results for: eng
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Plasmid#129048Purposeinactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA8 targeting human TSDRDepositorInsertdCas9-hudTET1CD, SgRNA8 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-1
Plasmid#129041Purposeinactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA1 targeting human TSDRDepositorInsertdCas9-hudTET1CD, SgRNA1 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLY152
Plasmid#130950Purposedcas9 generator and reporter circuit (PpspA-2G6 with sfgfp::ASV)DepositorInsertsdcas9
tetR
sfgfp
UseSynthetic BiologyTagsASV tagExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterPpspA-2G6 and PtetAvailable SinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CAG-NOTCH2NL-delta EGF repeats (N2NL-delta EGF)-ires-EGFP
Plasmid#122955PurposeLentiviral expression of NOTCH2NL delta EGF repeats under CAG promoter with EGFP expressionDepositorInsertNOTCH2NL-delta EGF repeats (NOTCH2NLB Human)
UseLentiviralTags6xhis tagMutationdeletion of EGF repeats from full length NOTCH2NLBPromoterCAG promoterAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(AsBE1.1) (RTW1351)
Plasmid#114079PurposeCAG promoter expression plasmid for human codon optimized AsBE1.1DepositorInserthuman codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.1)
TagsSV40 NLSExpressionMammalianAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Rb-379-928Δ738-775-pGex2T
Plasmid#21783DepositorInsertRb (RB1 Human)
TagsGSTExpressionBacterialMutationdeletion mutant ORF contains aa 379-928D737-774 o…Available SinceSept. 30, 2009AvailabilityAcademic Institutions and Nonprofits only -
TtA_pET29b
Plasmid#215432PurposeExpression construct for TtA gene, codon optimized for expression in E. coliDepositorInsertTtA
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC038
Plasmid#156345PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyMutationWT antiRBS engineered to orthogonal antiRBS, PS e…Available SinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRF033d
Plasmid#156354PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyMutationWT antiRBS engineered to orthogonal antiRBS, PS e…Available SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRF031d
Plasmid#156352PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyMutationWT antiRBS engineered to orthogonal antiRBS, PS e…Available SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC019
Plasmid#156350PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyMutationWT antiRBS engineered to orthogonal antiRBS, PS e…Available SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRF034d
Plasmid#156355PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyMutationWT antiRBS engineered to orthogonal antiRBS, PS e…Available SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRF028d
Plasmid#156349PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyMutationWT antiRBS engineered to orthogonal antiRBS, PS e…Available SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRF035d
Plasmid#156356PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyMutationWT antiRBS engineered to orthogonal antiRBS, PS e…Available SinceDec. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRF030d
Plasmid#156351PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyMutationWT antiRBS engineered to orthogonal antiRBS, PS e…Available SinceDec. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC041
Plasmid#156348PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyMutationWT antiRBS engineered to orthogonal antiRBS, PS e…Available SinceDec. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC040
Plasmid#156346PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyMutationWT antiRBS engineered to orthogonal antiRBS, PS e…Available SinceDec. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRF036d
Plasmid#156357PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyMutationWT antiRBS engineered to orthogonal antiRBS, PS e…Available SinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC039
Plasmid#156347PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyMutationWT antiRBS engineered to orthogonal antiRBS, PS e…Available SinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC042
Plasmid#156344PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyMutationWT antiRBS engineered to orthogonal antiRBS, PS e…Available SinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only