We narrowed to 4,705 results for: golden gate
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Plasmid#104440PurposeProvide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005DepositorInsertsgRNA-transRNA transcription by U6 promoter
UseCRISPRExpressionBacterial and PlantPromoterU6 promoterAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
TL1 alphaL_DdCBE 3-4 (left, DddA11-C) - TL1 alphaDdCBE 1 (left, DddA11-C)
Plasmid#221998PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alphaL-3/4 left, alpha-1 left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233A | DddA: T1413IPromoterCMVAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICSL11055
Plasmid#68252PurposeLevel 1 Golden Gate Cassette: Plant kanamycin resistance cassetteDepositorInsertPromoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos
UseSynthetic BiologyExpressionPlantAvailable SinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHS1871
Plasmid#205976PurposeU6-JAGBLH010000220 Cas8-HNH DR-BpiI-DRDepositorInsertCas8-HNH DR-BpiI-DR
ExpressionMammalianPromoterU6Available SinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGT6471
Plasmid#219428PurposeModule of intronized NLS-SpCas9-NLS for N-terminal and C-terminal tag fusions (AGGT_N-SpCas9i-N_TTCG)DepositorInsertAGGT_NLS-SpCas9i-NLS(no stop)_TTCG
UseMoclo compatible level 0 moduleAvailable SinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pN-iRS3GG
Plasmid#98858PurposeExpresses pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).DepositorInsertPheS cassette to be selectively replaced by unique asRNA probes
ExpressionBacterialPromoterpBADAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpyCas9-E566-LOV-C450A-D432V(M519)
Plasmid#250779PurposePotent temperature-deactivated SpyCas9DepositorInsertSpyCas9 with an AsLOV2 insertion behind E566 and C450A and D432V mutation
UseCRISPRTags3xFLAGExpressionMammalianMutationC450A, D432VAvailable SinceMarch 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
AraC-S170-LOV-C450A-K488N(M393)
Plasmid#250777PurposeExpresses AraC-S170-LOV from a Trc promoter. For use in E. coliDepositorInsertAraC with an AsLOV2 insertion behind S170 and C450A and K488N mutation
UseSynthetic BiologyTagsHis-tagExpressionBacterialMutationC450A, K488NAvailable SinceMarch 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
AraC-S170-LOV-C450A-D432V(M392)
Plasmid#250776PurposeExpresses AraC-S170-LOV from a Trc promoter. For use in E. coliDepositorInsertAraC with an AsLOV2 insertion behind S170 and C450A and D432V mutation
UseSynthetic BiologyTagsHis-tagExpressionBacterialMutationC450A, D432VAvailable SinceMarch 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132B-TLS-CsALSgR1.1
Plasmid#245877PurposeGolden gate entry vector carrying the 1st gRNA for base editing in Carrizo citrange ALS gene along with TLS mobile RNA sequenceDepositorInsertCsALSgR1.1
UseCRISPRExpressionPlantPromoterAtU3Available SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only