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BRAF gRNA (BRDN0001146266)

Plasmid

#76480

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRAF

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRAF (BRAF Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AKT1 gRNA (BRDN0001149266)

Plasmid

#75501

Purpose

3rd generation lentiviral gRNA plasmid targeting human AKT1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AKT1 (AKT1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BRD2 gRNA (BRDN0001148924)

Plasmid

#77984

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRD2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRD2 (BRD2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EIF2AK2 gRNA (BRDN0001148668)

Plasmid

#75639

Purpose

3rd generation lentiviral gRNA plasmid targeting human EIF2AK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EIF2AK2 (EIF2AK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FIP1L1

Plasmid

#155605

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

FIP1L1 (FIP1L1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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CDK4 gRNA (BRDN0001145730)

Plasmid

#76176

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK4 (CDK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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CHUK gRNA (BRDN0001149372)

Plasmid

#77033

Purpose

3rd generation lentiviral gRNA plasmid targeting human CHUK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CHUK (CHUK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CHUK gRNA (BRDN0001149269)

Plasmid

#77034

Purpose

3rd generation lentiviral gRNA plasmid targeting human CHUK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CHUK (CHUK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MET gRNA (BRDN0001147560)

Plasmid

#76061

Purpose

3rd generation lentiviral gRNA plasmid targeting human MET

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MET (MET Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EGFR gRNA (BRDN0001149464)

Plasmid

#75691

Purpose

3rd generation lentiviral gRNA plasmid targeting human EGFR

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EGFR (EGFR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BRD4 gRNA (BRDN0001145474)

Plasmid

#77347

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRD4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRD4 (BRD4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MARK2 gRNA (BRDN0001145542)

Plasmid

#77591

Purpose

3rd generation lentiviral gRNA plasmid targeting human MARK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MARK2 (MARK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.GFAP.SF-iGluSnFR.S72A

Plasmid

#106194

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Mutation

GltI: S72A

Promoter

GFAP

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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KSR1 gRNA (BRDN0001146869)

Plasmid

#77858

Purpose

3rd generation lentiviral gRNA plasmid targeting human KSR1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

KSR1 (KSR1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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KSR1 gRNA (BRDN0001146294)

Plasmid

#77859

Purpose

3rd generation lentiviral gRNA plasmid targeting human KSR1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

KSR1 (KSR1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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KSR1 gRNA (BRDN0001147076)

Plasmid

#77860

Purpose

3rd generation lentiviral gRNA plasmid targeting human KSR1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

KSR1 (KSR1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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KSR2 gRNA (BRDN0001145237)

Plasmid

#76427

Purpose

3rd generation lentiviral gRNA plasmid targeting human KSR2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

KSR2 (KSR2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
KSR2 gRNA (BRDN0001147424)

Plasmid

#76425

Purpose

3rd generation lentiviral gRNA plasmid targeting human KSR2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

KSR2 (KSR2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
KSR2 gRNA (BRDN0001148172)

Plasmid

#76426

Purpose

3rd generation lentiviral gRNA plasmid targeting human KSR2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

KSR2 (KSR2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MARK3 gRNA (BRDN0001145035)

Plasmid

#77716

Purpose

3rd generation lentiviral gRNA plasmid targeting human MARK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MARK3 (MARK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge