We narrowed to 18,671 results for: Met;

Showing: 8441 - 8460 of 18671 results

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GST-RB (654-752)

Plasmid

#31496

Depositor

Julien Sage

Article

Saddic et al J Biol Chem. 2010 Nov 26. 285(48):37733-

Insert

RB (RB1 Human)

Tags

GST

Expression

Bacterial

Mutation

Contains amino acids 654-752

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA5/FRT-ChemoB-NAD

Plasmid

#193824

Purpose

CMV overexpression of ChemoB-NAD in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoB-NAD

Expression

Mammalian

Mutation

EBFP2:N39Y, A206K, T225R; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3_cAMPFIRE-M

Plasmid

#182280

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-M (cAMP sensor)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pSF278

Plasmid

#167673

Purpose

Intermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU295

Plasmid

#167694

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro EGFP cLAP +2

Plasmid

#194310

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLap(EGFP)-P2A-Hygro

Use

Bacterial cloning vector

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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4xHRE-MinTK-CRE

Plasmid

#141144

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

4xHRE-MinTK-CRE

Use

Lentiviral

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pmScarlet3-Peroxi_C1

Plasmid

#189769

Purpose

Construct for labelling peroxisomes in mammalian cells with peroxisomal targeted mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-Peroxi

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pSF463

Plasmid

#167671

Purpose

Intermediate vector for two gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-eGFP

Plasmid

#157733

Purpose

mammalian expression of untagged eGFP under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

eGFP

Expression

Mammalian

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pSF464

Plasmid

#167672

Purpose

Intermediate vector for four gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-enCjCas9-BlastR

Plasmid

#220978

Purpose

Expresses human codon-optimized enCjCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

enCjCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pPAmCherry2-C1

Plasmid

#31932

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry2

Expression

Mammalian

Mutation

M18L/K69N/L84F/A147T/S148L/I165L/Q167A/L169T/A179…

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pdCas9-Tet1-CD

Plasmid

#83340

Purpose

To introduce dCas9-fused Tet1-CD and sgRNA2.0 system

Depositor

Ronggui Hu

Article

Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

Insert

dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

U6, CBH

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCG-TPMV Fd32

Plasmid

#163964

Purpose

Expression of TPMV F glycoprotein in mammalian cells

Depositor

Jakob Reiser

Article

Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

Insert

TPMV F glycoprotein

Expression

Mammalian

Mutation

Lacking 32 amino acids at C-terminus

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pMD1 anti-GFP nanobody-Tn5

Plasmid

#215567

Purpose

Expresses anti-GFP nanobody-Tn5 fusion in bacteria with an N-terminal 3xFlag tag and a C-terminal intein-CBD-intein tag

Depositor

Isabelle Mansuy

Article

Maria A. et al bioRxiv 2024.07.12.603224

Insert

anti-GFP nanobody-Tn5 fusion

Tags

3xFlag and intein-CBD

Expression

Bacterial

Promoter

T7

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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MSCVhygro-F-Ezh2

Plasmid

#24926

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

Ezh2 (Ezh2 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

June 27, 2010

Availability

Academic Institutions and Nonprofits only

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pVH607-Tier1-PCREm4-Nluc

Plasmid

#169594

Purpose

Tier-1 vector encoding PCREm4-driven Nluc expression (PCREm4-Nluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

cAMP response element-controlled NanoLuc Luciferase expression cassette

Expression

Mammalian

Promoter

PCREm4

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCm C-term MBP NikJC199U

Plasmid

#174365

Purpose

Expression plasmid with a TEV removable C-term 8xHis tagged MBP tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

Depositor

Brandon Greene

Article

Caceres et al Methods Enzymol. 2022;662:119-141. doi:

Insert

nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]

Tags

8xHis, MBP, and TEV

Expression

Bacterial

Mutation

C199U

Promoter

T7

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-hG9a

Plasmid

#33025

Depositor

Martin Walsh

Article

Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

Insert

hG9a (EHMT2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2012

Availability

Academic Institutions and Nonprofits only

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