We narrowed to 9,182 results for: Pol

1. RcSxph D785N

   Plasmid

   #194036

   Purpose

   Protein expression and characterization

   Depositor

   Dan Minor

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

   Insert

   Rana catesbeiana Saxiphilin
   

   Tags

   His10 and mEGFP,

   Expression

   Insect

   Mutation

   D785N

   Promoter

   Polyhedrin

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. RcSxph Q787A

   Plasmid

   #194037

   Purpose

   Protein expression and characterizatio

   Depositor

   Dan Minor

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

   Insert

   Rana catesbeiana Saxiphilin
   

   Tags

   His10 and mEGFP,

   Expression

   Insect

   Mutation

   Q787A

   Promoter

   Polyhedrin

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. RcSxph Q787E

   Plasmid

   #194038

   Purpose

   Protein expression and characterization

   Depositor

   Dan Minor

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

   Insert

   Rana catesbeiana Saxiphilin
   

   Tags

   His10 and mEGFP,

   Expression

   Insect

   Mutation

   Q787E

   Promoter

   Polyhedrin

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. RcSxph K789A

   Plasmid

   #194039

   Purpose

   Protein expression and characterization

   Depositor

   Dan Minor

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

   Insert

   Rana catesbeiana Saxiphilin
   

   Tags

   His10 and mEGFP,

   Expression

   Insect

   Mutation

   K789A

   Promoter

   Polyhedrin

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. RcSxph D794A

   Plasmid

   #194040

   Purpose

   Protein expression and characterization

   Depositor

   Dan Minor

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

   Insert

   Rana catesbeiana Saxiphilin
   

   Tags

   His10 and mEGFP,

   Expression

   Insect

   Mutation

   D794A

   Promoter

   Polyhedrin

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. RcSxph D794E

   Plasmid

   #194041

   Purpose

   Protein expression and characterization

   Depositor

   Dan Minor

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

   Insert

   Rana catesbeiana Saxiphilin
   

   Tags

   His10 and mEGFP,

   Expression

   Insect

   Mutation

   D794E

   Promoter

   Polyhedrin

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RcSxph D794N

   Plasmid

   #194042

   Purpose

   Protein expression and characterization

   Depositor

   Dan Minor

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

   Insert

   Rana catesbeiana Saxiphilin
   

   Tags

   His10 and mEGFP,

   Expression

   Insect

   Mutation

   D794N

   Promoter

   Polyhedrin

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. RcSxph Y795A

   Plasmid

   #194043

   Purpose

   Protein expression and characterization

   Depositor

   Dan Minor

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

   Insert

   Rana catesbeiana Saxiphilin
   

   Tags

   His10 and mEGFP,

   Expression

   Insect

   Mutation

   Y795A

   Promoter

   Polyhedrin

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. RcSxph Y558A/I782A

   Plasmid

   #194044

   Purpose

   Protein expression and characterization

   Depositor

   Dan Minor

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

   Insert

   Rana catesbeiana Saxiphilin
   

   Tags

   His10 and mEGFP,

   Expression

   Insect

   Mutation

   Y558A/I782A

   Promoter

   Polyhedrin

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mFzd4CRDL(42-203)-6xHis_pAcGP67a

    Plasmid

    #216379

    Purpose

    Baculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cells

    Depositor

    William Weis

    Article

    Bruguera et al bioRxiv 2024.02.03.578714

    Insert

    Frizzled-4 (Fzd4 Mouse)
    

    Use

    Baculovirus

    Tags

    6xHis and GP64 signal sequence

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUC57-ITR2-H1- sgCTG Merry-eCMV-SaSpD10A+linker (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #210736

    Purpose

    Coding for SaSp D10A Cas9 alongside Merry sgRNA targeting CAG repeats

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp D10A Cas9
    Merry sgCAG
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    D10A, N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available Since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC57-ITR2-H1- sgCTG Gollum-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #210735

    Purpose

    Coding for SaSp D10A Cas9 alongside Gollum sgRNA targeting CAG repeats

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp D10A Cas9
    Gollum sgCAG
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    D10A, N terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available Since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLV-SFFV-Llgl1-WPRE-UbC-Emerald

    Plasmid

    #203805

    Purpose

    Lentiviral vector plasmid expressing mouse Llgl1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Inserts

    Llgl1 (Llgl1 Mouse)
    Emerald
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC57-min5UTR-hRluc-A60

    Plasmid

    #182638

    Purpose

    Transcription template plasmid for a minimal 5'UTR followed by Renilla luciferase open reading frame and polyA sequence

    Depositor

    Stephen Floor

    Article

    Calviello et al Nucleic Acids Res. 2021 May 21;49(9):533

    Insert

    Renilla luciferase,
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGGS-C-TEV-HAT

    Plasmid

    #202597

    Purpose

    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Type

    Empty backbone

    Tags

    TEV cleavable site (ENLYFQG) and poly-histidine a…

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGGS-N-TEV-HAT

    Plasmid

    #202598

    Purpose

    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Type

    Empty backbone

    Tags

    TEV cleavable site (ENLYFQG) and poly-histidine a…

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FAK Fus

    Plasmid

    #186145

    Purpose

    For expression of recombinant FAK using baculovirus in SF9 cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Tags

    His6

    Expression

    Insect

    Mutation

    C-terminal linker swapped with FUS IDR

    Promoter

    polyhedrin promoter

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFastBac FAK W266A

    Plasmid

    #186144

    Purpose

    For expresseion of recombinant FAK using baccolovirus in SF9 cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Tags

    His6

    Expression

    Insect

    Mutation

    DNA mutation T796G;G797C Amino acid mutation W266A

    Promoter

    polyhedrin promoter

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lck

    Plasmid

    #186130

    Purpose

    For expression of recombinant Lck in SF9 cells.

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    Lck (Lck Mouse)
    

    Tags

    His6

    Expression

    Insect

    Mutation

    A1687T

    Promoter

    polyhedrin promoter

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FAK Pyk2

    Plasmid

    #186146

    Purpose

    For expresseion of recombinant FAK using baccolovirus in SF9 cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Tags

    His6

    Expression

    Insect

    Mutation

    C-terminal linker swapped with Pyk2 linker

    Promoter

    polyhedrin promoter

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge