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PEAK1 gRNA (BRDN0001147666)

Plasmid

#75855

Purpose

3rd generation lentiviral gRNA plasmid targeting human PEAK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PEAK1 (PEAK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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JAK2 gRNA (BRDN0001146541)

Plasmid

#75726

Purpose

3rd generation lentiviral gRNA plasmid targeting human JAK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

JAK2 (JAK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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VRK1 gRNA (BRDN0001147568)

Plasmid

#75522

Purpose

3rd generation lentiviral gRNA plasmid targeting human VRK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

VRK1 (VRK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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VRK1 gRNA (BRDN0001148483)

Plasmid

#75523

Purpose

3rd generation lentiviral gRNA plasmid targeting human VRK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

VRK1 (VRK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PKMYT1 gRNA (BRDN0001146095)

Plasmid

#77278

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKMYT1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKMYT1 (PKMYT1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 30, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-GFP

Plasmid

#75130

Purpose

Retroviral expression plasmid encoding shBrd9_1116 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1116

Use

Retroviral

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-Ago2-FDM4

Plasmid

#72211

Purpose

Mammalian expression of aa 373-860 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Δ 1-372

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
hPlekhm1_pIRES puro Glue

Plasmid

#73453

Purpose

Express human Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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-161+80pCalb2/E2F2/NRF-1(-64)-like

Plasmid

#66742

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CGCG(-64) to TACA

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-TER+-shLKB1-Ms

Plasmid

#61234

Purpose

Entry clone encoding shRNA to mouse LKB1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

shLkb1 (Ms/Hu) (STK11, Stk11 Mouse, Human)

Use

Entr clone

Promoter

H1/TO

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pDEST myc MOV10 K530A

Plasmid

#51719

Purpose

plasmid expresses myc-tagged MOV10 K530A

Depositor

Markus Landthaler

Article

Gregersen et al Mol Cell. 2014 May 22;54(4):573-85. doi:

Insert

MOV10 K530A (MOV10 Human)

Tags

myc

Expression

Mammalian

Mutation

K530A

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-TRP1-PGAL1-GST

Plasmid

#41612

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

GST, TRP1 reverse orientation, and yeast ADH1 ter…

Available Since

Dec. 11, 2012

Availability

Academic Institutions and Nonprofits only

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KHBD00476

Plasmid

#39585

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5838 (Dref Fly)

Use

Gateway Cloning

Promoter

none

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
p177 Dync1i2.C (Ex 1b)

Plasmid

#26445

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform C (exon 1b) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
p196 Dync1i2.A (Ex 1a)

Plasmid

#26446

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform A (exon 1a) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
p169 Dync1i1.C

Plasmid

#26440

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 1 isoform C (Dync1i1 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
histamine receptor H3 (hrh3)_L (OZ549)

Plasmid

#28074

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting histamine receptor H3 (hrh3) (hrh3 Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
apoeb (apolipoprotein Eb)_L (OZ529)

Plasmid

#27206

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting apoeb (apoeb Zebrafish)

Use

Zebrafish targeting

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
apoeb (apolipoprotein Eb)_R (OZ530)

Plasmid

#27207

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting apoeb (apoeb Zebrafish)

Use

Zebrafish targeting

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
VDAC2_L (OZ523)

Plasmid

#27198

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting VDAC2 (vdac2 Zebrafish)

Use

Zebrafish targeting

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only